MicroRNAs in Idiopathic Childhood Nephrotic Syndrome

Lorenzen, Johan M.; Thum, Thomas

doi:10.1373/clinchem.2013.202671

Cited by 4 publications

(4 citation statements)

References 17 publications

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“…A set of miRNAs that was dysregulated in the kidney tissues of children with nephropathy and was related to the pathogenesis of NS in children (Lu et al, 2015). Moreover, these miRNAs were also significantly increased in the serum or urine of children with NS and markedly decreased with the clinical remission of the patients, indicating that this specific set of circulating or urinary miRNAs could serve as a promising and noninvasive means in monitoring and stratifying children with severe complications (Lorenzen & Thum, 2013; Lu et al, 2015).…”

Section: Circulating and Urinary Mirnasmentioning

confidence: 99%

MicroRNAs in childhood nephrotic syndrome

Dhandapani

Venkatesan

Charmine

2021

Journal Cellular Physiology

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The discovery of microRNAs (miRNAs) has opened up new avenues of research to understand the molecular basis of a number of diseases. Because of their conservative feature in evolution and important role in the physiological function, microRNAs could be treated as predictors for disease classification and clinical process based on the specific expression. The identification of novel miRNAs and their target genes can be considered as potential targets for novel drugs. Furthermore, currently, the circulatory and urinary exosomal miRNAs are gaining increasing attention as their expression profiles are often associated with specific diseases, and they exhibit great potential as noninvasive or minimally invasive biomarkers for the diagnosis of various diseases. The remarkable stability of these extracellular miRNAs circulating in the blood or excreted in the urine underscored their key importance as biomarkers of certain diseases. There is voluminous literature concerning the role of microRNAs in other diseases, such as cardiovascular diseases, diabetic nephropathy, and so forth. However, little is known about their diagnostic ability for the pediatric nephrotic syndrome (NS). The present review article highlights the recent advances in the role of miRNAs in the pathogenesis and molecular basis of NS with an aim to bring new insights into further research applications for the development of new therapeutic agents for NS.

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Section: Circulating and Urinary Mirnasmentioning

confidence: 99%

MicroRNAs in childhood nephrotic syndrome

Dhandapani

Venkatesan

Charmine

2021

Journal Cellular Physiology

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“…In EBioMedicine , Zhang et al explored the clinical value of urinary exosomal miRNAs in children with idiopathic nephrotic syndrome [6]. In a previous manuscript, they reported 5 miRNAs increased in the serum and 1 in the urine (miRNA-30a-5p) in children with idiopathic NS compared to controls [7,8]. In the present study, urine samples were collected in 129 children with NS and in 126 age-sex matched healthy controls.…”

mentioning

confidence: 93%

Are microRNA potential biomarkers in children with idiopathic nephrotic syndrome?

Parvex

2019

EBioMedicine

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“…At present, the primary symptoms are severe proteinuria and hypoalbuminemia, as well as hyperlipidaemia and oedema, which are major clinical diagnostic indicators, but these symptoms may not be accurate predictors for patient outcome because of symptom heterogeneity and complications [5,6]. Renal biopsy, the standard method to judge pathological type and prognosis, is an invasive operation with latent risk and is normally not feasible for serial monitoring, especially in children [7,8]. Moreover, misdiagnosis may be caused by improper tissue sampling [9].…”

Section: Introductionmentioning

confidence: 99%

“…We previously found a set of miRNAs that was dysregulated in the kidney tissues of children with nephropathy and was related to the pathogenesis of nephrosis in children [11]. Moreover, these miRNAs were also significantly increased in the serum or urine of children with NS and markedly decreased with the clinical remission of the patients, indicating that this specific set of circulating or urinary miRNAs could serve as a promising and non-invasive means in monitoring and stratifying children with severe complications [7,8].…”

Section: Introductionmentioning

confidence: 99%

Increased urinary exosomal microRNAs in children with idiopathic nephrotic syndrome

Chen

Wang

et al. 2019

EBioMedicine

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BackgroundUrinary exosomal miRNAs are gaining increasing attention for their potential as ideal non-invasive biomarkers for kidney diseases; however, little is known about their diagnostic ability for paediatric nephrotic syndrome (NS). This study explored the clinical value of urinary exosomal miRNAs for paediatric idiopathic NS.MethodsUrine samples were collected from 129 NS children and 126 age−/sex-matched healthy controls. The miRNA profile of urinary exosomes was analysed by high-throughput Illumina sequencing via synthesis (SBS) technology followed by verification with a quantitative reverse-transcription polymerase chain reaction (RT-qPCR) assay arranged in two independent cohorts. Additionally, paired urine samples from 65 of these patients were collected before and after treatment.FindingsThe Illumina SBS identified 30 markedly increased urinary exosomal miRNAs in NS children compared with controls (≥ 5-fold, P < .05). Fifteen miRNAs were selected for further investigation, of which 5 (miR-194-5p, miR-146b-5p, miR-378a-3p, miR-23b-3p and miR-30a-5p) were verified by RT-qPCR to be significantly and steadily increased in NS (> 3-fold, P < .01) and markedly reduced during the clinical remission period (P < .001). Moreover, the concentrations of miR-194-5p and miR-23b-3p were significantly positively correlated with the urine protein content and were markedly higher in the high urine protein group than in the low urine protein group (P < .001 and P < .01, respectively).InterpretationsWe identified 5 altered urinary exosomal miRNAs in NS children with disease progression and treatment. These urinary exosomal miRNAs could be promising and non-invasive potential biomarker candidates for diagnosing, monitoring and stratifying paediatric NS.FundNational Natural Science Foundation of China; Fund of State Key Laboratory of Analytical Chemistry for Life Science; National Basic Research Programme of China; Foundation of Jiangsu Provincial Medical Youth Talent.

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MicroRNAs in Idiopathic Childhood Nephrotic Syndrome

Cited by 4 publications

References 17 publications

MicroRNAs in childhood nephrotic syndrome

MicroRNAs in childhood nephrotic syndrome

Are microRNA potential biomarkers in children with idiopathic nephrotic syndrome?

Increased urinary exosomal microRNAs in children with idiopathic nephrotic syndrome

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