MicroRNAs in Ovarian Cancer

Katz, Betina; Tropé, Claes G.; Reich, Reuven; Davidson, Ben

doi:10.1016/j.humpath.2015.06.013

Cited by 52 publications

(37 citation statements)

References 106 publications

(148 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Some miRs that have been found altered in ovarian cancer include Let-7, miR-200 family, miR-17-92, miR-21, miR-145 and miR-23b [112,113,114]. Most of them have also been studied in other types of cancer, and they can have different roles depending on the cancer type.…”

Section: Role Of Mirs In Ovarian Cancermentioning

confidence: 99%

Role of Nerve Growth Factor (NGF) and miRNAs in Epithelial Ovarian Cancer

Retamales-Ortega

Oróstica

Vera

et al. 2017

IJMS

View full text Add to dashboard Cite

Ovarian cancer is the eighth most common cancer in women worldwide, and epithelial ovarian cancer (EOC) represents 90% of cases. Nerve growth factor (NGF) and its high affinity receptor tyrosine kinase A receptor (TRKA) have been associated with the development of several types of cancer, including EOC; both NGF and TRKA levels are elevated in this pathology. EOC presents high angiogenesis and several molecules have been reported to induce this process. NGF increases angiogenesis through its TRKA receptor on endothelial cells, and by indirectly inducing vascular endothelial growth factor expression. Other molecules controlled by NGF include ciclooxigenase-2, disintegrin and metalloproteinase domain-containing protein 17 (ADAM17) and calreticulin (CRT), proteins involved in crucial processes needed for EOC progression. These molecules could be modified through microRNA regulation, which could be regulated by NGF. MicroRNAs are the widest family of non-coding RNAs; they bind to 3′-UTR of mRNAs to inhibit their translation, to deadenilate or to degraded them. In EOC, a deregulation in microRNA expression has been described, including alterations of miR-200 family, cluster-17-92, and miR-23b, among others. Since the NGF-microRNA relationship in pathologies has not been studied, this review proposes that some microRNAs could be associated with NGF/TRKA activation, modifying protein levels needed for EOC progression.

show abstract

Section: Role Of Mirs In Ovarian Cancermentioning

confidence: 99%

Role of Nerve Growth Factor (NGF) and miRNAs in Epithelial Ovarian Cancer

Retamales-Ortega

Oróstica

Vera

et al. 2017

IJMS

View full text Add to dashboard Cite

show abstract

“…An increasing number of studies have suggested that the initiation and development of EOC may be associated with microRNA-494 is a potential prognostic marker and inhibits cellular proliferation, migration and invasion by targeting SIRT1 in epithelial ovarian cancer AIJUN YANG 1* , XUENAN WANG 1* , CHUNNA YU 1 , ZHENZHEN JIN multistep changes in the genome, in particular abnormal expression of microRNA (miRNA/miR) (10)(11)(12). miRNAs are a large family of non-protein-coding short RNA molecules with 18-25 nucleotides that were first identified in 1993 by Lee et al (13).…”

Section: Introductionmentioning

confidence: 99%

microRNA-494 is a potential prognostic marker and inhibits cellular proliferation, migration and invasion by targeting SIRT1 in epithelial ovarian cancer

Yang

Wang

et al. 2017

Oncology Letters

View full text Add to dashboard Cite

Abstract. Ovarian cancer is one of the most common types of gynecological malignancy worldwide, and is the fourth leading cause of cancer-associated mortality among women. Despite improvements in therapeutic treatments, the prognosis for epithelial ovarian cancer (EOC) remains poor, mainly due to the rapid growth and metastasis of ovarian cancer tumors. An increasing number of studies have indicated that microRNAs (miRNAs) are involved in the carcinogenesis and progression of human cancer, suggesting that miRNAs may be used in clinical prognosis and as a therapeutic target in EOC. The aim of the present study was to investigate the expression levels of miRNA-494 in EOC tissues and cell lines. The clinical significance of miRNA-494 in patients with EOC was also evaluated. The results demonstrated that miRNA-494 was significantly downregulated in EOC tissues and cell lines. Low expression levels of miRNA-494 were associated with poor prognostic features, including International Federation of Gynecology and Obstetrics stage, tumor size and lymph node metastasis. In vitro functional studies demonstrated that overexpression of miRNA-494 inhibited proliferation, migration and invasion in EOC cells. By contrast, knockdown of miRNA-494 enhanced cell growth, migration and invasion in EOC cells. Notably, sirtuin 1 (SIRT1) was identified as a direct target of miRNA-494 in EOC. Furthermore, MTT, cell migration and invasion assays verified that EOC cell proliferation, migration and invasion were completely restored with forced miRNA-494 expression and SIRT1 restoration. Together, these findings suggest that miRNA-494 is a potential prognostic marker, and may provide novel therapeutic regimens of targeted therapy for EOC.

show abstract

“…A previous study revealed that the expression profiles of miRNAs in tumor tissue and normal tissue exhibit significant differences, and the expression levels of miRNAs are associated with the development, differentiation, metastasis and prognosis of tumor cells (25). Previous studies have analyzed miRNA expression profiles in order to investigate miRNAs in a variety of malignant tumors, including OC and OC cell lines, expecting to identify miRNAs with diagnostic potential (26,27). Zhang et al (28) detected the expression of 173 mature miRNAs in OC cell lines and human ovarian surface epithelial cell lines, and identified 35 miRNAs with significant expression differences, among which 31 were downregulated and four were upregulated.…”

Section: Discussionmentioning

confidence: 99%

Effects of let‑7c on the proliferation of ovarian carcinoma cells by targeted regulation of CDC25a gene expression

et al. 2018

View full text Add to dashboard Cite

Abstract. MicroRNAs serve a role in the development of ovarian cancer (OC). The present study investigated whether let-7c is able to regulate the proliferation of OC cells by targeting cell division cycle 25A (CDC25a). The reverse transcription-quantitative polymerase chain reaction was performed to detect the expression of let-7c in OC specimens. Let-7c agomir was transfected into OC cells, and the proliferation and apoptosis of OC cells were detected. A dual-luciferase assay and western blotting were performed to analyze whether CDC25a was the target gene of let-7c as well as its interaction site. The results revealed that, in OC tissue, let-7c was downregulated when compared with normal ovarian tissue. A Cell Counting Kit-8 (CCK8) assay, colony formation assay and flow cytometry demonstrated that increased expression of let-7c was able to inhibit the proliferation and increase the apoptosis of OC cells. Western blotting revealed that upregulated let-7c is able to decrease the expression of CDC25a, and a dual-luciferase assay and a recovery assay demonstrated that let-7c was able to regulate the expression of the 3' untranslated region of CDC25a. Therefore, the roles of let-7c in inhibiting the proliferation and promoting the apoptosis of OC cells may be realized through the regulation of the expression of CDC25a. The results of the present study revealed that let-7c may be a novel target in the diagnosis and treatment of OC.

show abstract

MicroRNAs in Ovarian Cancer

Cited by 52 publications

References 106 publications

Role of Nerve Growth Factor (NGF) and miRNAs in Epithelial Ovarian Cancer

Role of Nerve Growth Factor (NGF) and miRNAs in Epithelial Ovarian Cancer

microRNA-494 is a potential prognostic marker and inhibits cellular proliferation, migration and invasion by targeting SIRT1 in epithelial ovarian cancer

Effects of let‑7c on the proliferation of ovarian carcinoma cells by targeted regulation of CDC25a gene expression

Contact Info

Product

Resources

About