Microsatellite Marker-Based Genetic Analysis of Relatedness Between Commercial and Heritage Turkeys (Meleagris gallopavo)

Kamara, D.; Gyenai, Kwaku B.; Geng, Tuoyu; Hammade, H.; Smith, Edward J.

doi:10.1093/ps/86.1.46

Cited by 26 publications

(20 citation statements)

References 14 publications

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“…However, this was relatively low when compared to the generally reported number of alleles per locus for microsatellite loci in the literature, which usually ranged from two to more than 10, suggesting low levels of allelic diversity for the local populations of P. viridis. The MetaPhor ® agarose gels that we used to type the microsatellite loci in this study had been shown by Ochsenreither et al (2006) to be as efficient as polyacrylamide gels and an automated capillary sequencer system (CEQ 8000; Beckman Coulter, Fullerton, USA) and by Kamara et al (2007) to be comparable with the ABI PRISM 3100 Genetic Analyzer (Applied Biosystems, Foster City, USA) for microsatellite allele discrimination.…”

Section: Discussionmentioning

confidence: 99%

Genetic characterization of Perna viridis L. in peninsular Malaysia using microsatellite markers

Ong

Yusoff

Yap

et al. 2009

J Genet

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A total of 19 polymorphic microsatellite loci were used to analyse levels of genetic variation for 10 populations of Perna viridis L. collected from all over peninsular Malaysia. The populations involved in this study included Pulau Aman in Penang, Tanjung Rhu in Kedah, Bagan Tiang in Perak, Pulau Ketam in Selangor, Muar, Parit Jawa, Pantai Lido and Kampung Pasir Puteh in Johore, and Kuala Pontian and Nenasi in Pahang state. The number of alleles per locus ranged from two to seven, with an average of 3.1. Heterozygote deficiencies were observed across all the 10 populations. Characterization of the populations revealed that local populations of P. viridis in peninsular Malaysia were genetically similar enough to be used as a biomonitoring agent for heavy metal contamination in the Straits of Malacca. Cluster analysis grouped the P. viridis populations according to their geographical distributions with the exception of Parit Jawa. The analysis also revealed that P. viridis from the northern parts of peninsular Malaysia were found to be the most distant populations among the populations of mussels investigated and P. viridis from the eastern part of peninsular Malaysia were closer to the central and southern populations than to the northern populations.

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Section: Discussionmentioning

confidence: 99%

Genetic characterization of Perna viridis L. in peninsular Malaysia using microsatellite markers

Ong

Yusoff

Yap

et al. 2009

J Genet

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“…Higher number of alleles was observed using turkey microsatellites on the Roslin backcross families (Burt et al, 2003) and on commercial line (Reed et al, 2000(Reed et al, , 2002. In contrast, Kamara et al (2007) observed a low number of alleles in five heritage domestic populations using 10 polymorphic turkey microsatellites chosen from those identified by Burt et al (2003).…”

Section: Resultsmentioning

confidence: 84%

Feasibility Study on the FAO Chicken Microsatellite Panel to Assess Genetic Variability in the Turkey (Meleagris Gallopavo)

Colombo

Strillacci

Cozzi

et al. 2014

Italian Journal of Animal Science

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The aim of this work was to study the feasibility of the Food and Agriculture Organization (FAO) microsatellite panel developed for chickens to assess genetic variability in turkeys. Genomic DNA was extracted from a total of 37 blood samples collected from turkey of different breeds [15 Brianzolo (BR); 12 Colli Euganei (EU); 10 Nero d'Italia (NI)], and all 31 chicken microsatellite markers recommended by the FAO were tested. The results show that 22 chicken markers out of 31 suggested by FAO guidelines can be applied to turkey populations. In particular, the multiplex groups confirmed in the turkey were the Multiplex Master Mix 1 (ADL0268, ADL0278, LEI0094, MCW0216, MCW0248) and the Master Mix 2 (MCW0034, MCW0069, MCW0081, MCW0222, MCW0295), whereas 13 microsatellites were amplified only under single polymerase chain reaction (PCR) conditions. No PCR products were obtained for 9 markers (LEI0166, MCW0020, MCW0078, MCW0080, MCW0104, MCW0123, MCW0248, MCW0284 and MCW0330), which is 29% of the total markers used. A panel of 22 markers was used to assess genetic diversity in three turkey breeds and a total number of 63 alleles were found. Observed (Ho) and expected (He) heterozygosity and polymorphism information content (PIC) values for each microsatellite and the relative mean values were also calculated. The mean values were 0. 210, 0.250, 0.203 for Ho; 0.301, 0.348, 0.228 for He; and 0.265, 0.313, 0.199 for PIC in NI, BR and EU, respectively.

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“…Microsatellites, also known as simple sequence repeats (SSRs), are highly polymorphic and abundant genetic markers (Li et al, 2002). They were widely employed in population genetic studies of numerous species, including poultry (Cheng and Crittenden, 1994;Kamara et al, 2007). Here, we developed and characterized 8 novel microsatellite markers for Huoyan geese, which will be useful for future studies on the population genetic structure and conservation genetics in this species.…”

Section: Introductionmentioning

confidence: 99%

Development of eight novel microsatellite markers for Huoyan geese

Cao

Zhao

et al. 2014

Genet. Mol. Res.

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ABSTRACT. In this study, we isolated microsatellite DNA from the Huoyan goose genome with magnetic beads. As a result, 150 positive clones were identified, and 148 microsatellites were found. Among the 148 microsatellites, 69.6% were perfect, 17.6% were imperfect, and the rest were compound type (12.8%). Twenty microsatellite primers were used to screen 90 individuals from 3 Huoyan goose populations. Eight loci were polymorphic with a low number of alleles (2 to 4). The observed and expected heterozygosities ranged from 0.3556 to 1 and from 0.2923 to 0.6868, respectively. All the 8 polymorphic loci were in Hardy-Weinberg equilibrium. These molecular markers will be useful for future studies on population genetic structure and conservation genetics in Huoyan geese.

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Microsatellite Marker-Based Genetic Analysis of Relatedness Between Commercial and Heritage Turkeys (Meleagris gallopavo)

Cited by 26 publications

References 14 publications

Genetic characterization of Perna viridis L. in peninsular Malaysia using microsatellite markers

Genetic characterization of Perna viridis L. in peninsular Malaysia using microsatellite markers

Feasibility Study on the FAO Chicken Microsatellite Panel to Assess Genetic Variability in the Turkey (Meleagris Gallopavo)

Development of eight novel microsatellite markers for Huoyan geese

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