Microsatellite markers for genome analysis in Brassica. II. Assignment of rapeseed microsatellites to the A and C genomes and genetic mapping in Brassica oleracea L.

Saal, Bernhard; Plieske, Joerg; Hu, Jinguo; Quirós, Carlos F.; Struss, D.

doi:10.1007/s001220051699

Cited by 53 publications

(32 citation statements)

References 17 publications

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“…They are highly polymorphic, reproducible, co-dominant, PCR-based markers and are the most preferred one and thus considered to be very potent in genotype discrimination. It has been reported previously that SSR is an important tool for germplasm characterization in a variety of crops, including oilseed Brassica (Saal et al, 2001). A similar result regarding effectiveness of SSR markers in monitoring genetic diversity have also been reported by Hopkins et al, (2006) and Fu and Gugel, (2010).…”

Section: Cluster Analysis Based On Ssrssupporting

confidence: 74%

Screening and Estimation of Allelic Differentiation in Indian mustard Using SSR Markers for Background Selection

Singh¹,

Singh²,

Ambawat³

et al. 2017

Int.J.Curr.Microbiol.App.Sci

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Section: Cluster Analysis Based On Ssrssupporting

confidence: 74%

Screening and Estimation of Allelic Differentiation in Indian mustard Using SSR Markers for Background Selection

Singh¹,

Singh²,

Ambawat³

et al. 2017

Int.J.Curr.Microbiol.App.Sci

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“…Hitherto, the number of SSRs developed from Brassica has been small (Lowe et al 2002;Plieske and Struss 2001;Saal et al 2001;Suwabe et al 2002;SzewcMcFadden et al 1996;Westman and Kresovich 1999) and includes some originally transferred from A. thaliana (Westman and Kresovich 1998). This report describes the development and characteristics of 398 SSR markers derived from B. rapa, B. nigra, B. oleracea and B. napus, and includes details of the attrition rates encountered at each stage of development, together with surveys of genome specificity and polymorphism for successfully designed primer pairs.…”

Section: Introductionmentioning

confidence: 97%

“…The Brassicas are characterised by an extraordinary level of intraspecific morphological phenotypic variation, fixed by artificial selection to form various crop types. This feature seems to be accompanied by a high degree of genetic variation pervasive throughout the entire genome, which in turn, has enabled a number of dense RFLP-based genetic linkage maps to be assembled for most of the Brassica species (Howell et al 2002;Kowalski et al 1994;Lionneton et al 2002;Lombard et al 2001;Lydiate et al 1993;Parkin et al 1995;Saal et al 2001). Where common sets of RFLP probes and parental genotypes have been used, definitive allele matching is possible, which allows the formulation of integrated or comparative maps Sebastian et al 2000).…”

Section: Introductionmentioning

confidence: 97%

Efficient large-scale development of microsatellites for marker and mapping applications in Brassica crop species

Moule²,

et al. 2003

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A set of 398 simple sequence repeat markers (SSRs) have been developed and characterised for use with genetic studies of Brassica species. Small-insert (250-900 bp) genomic libraries from Brassica rapa, B. nigra, B. oleracea and B. napus, highly enriched for dinucleotide and trinucleotide SSR motifs, were constructed. Screening the clones with a mixture of oligonucleotide repeat probes revealed positive hybridisation to between 75% and 90% of the clones. Of these, 1230 were sequenced. Primer pairs were designed for 398 SSR clones, and of these, 270 (67.8%) amplified a PCR product of the expected size in their focal and/or closely related species. A further screen of 138 primers pairs that produced a PCR product in B. napus germplasm found that 86 (62.3%) revealed length polymorphisms within at least one line of a test array representing the four Brassica species. The results of this screen were used to identify 56 SSRs and were combined with 41 SSRs that had previously shown polymorphism between the parents of a B. napus mapping population. These 97 SSR markers were mapped relative to a framework of RFLP markers and detected 136 loci over all 19 linkage groups of the oilseed rape genome.

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“…Recently, simple sequence repeat (SSR) markers, consisting of short tandem base repeats (2-8 bp units), have gained increasing importance in plant variety testing (Cooke 1999;Donini et al 2000). SSRs were studied in several crop species including Brassicas (Kresovich et al 1995;Charters et al 1996;SzewcMcFadden et al 1996;Uzunova and Ecke 1999;Plieske and Strauss 2001;Saal et al 2001). These studies showed that SSRs are a valuable tool for characterising germplasm in Brassica species, because they are numerous, highly informative, technically simple, robust and suitable for automated allele detection and sizing (Rafalski and Tingey 1993).…”

Section: Introductionmentioning

confidence: 99%

The development of multiplex simple sequence repeat (SSR) markers to complement distinctness, uniformity and stability testing of rape (Brassica napus L.) varieties

et al. 2003

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To assess the potential of multiplex SSR markers for testing distinctness, uniformity and stability of rape (Brassica napus L.) varieties, we developed three multiplex SSR sets composed of five markers each. These were used to measure the extent of diversity within and between a set of ten varieties using a fluorescence-based semi-automated detection technology. Also, we evaluated the significance of any correlation between SSRs, pedigree and five of the morphological characters currently used for statutory distinctness, uniformity and stability testing of rape varieties. An assignment test was allowed to identify 99% of the plants examined, with the correct variety based on the analysis of 48 individual plants for each variety. Principal coordinate analysis confirmed that a high degree of separation between varieties could be achieved. Varieties were separated in three groups corresponding to winter, spring and forage types. These results suggested that it should be possible to select a set of markers for obtaining a suitable separation. Diversity within varieties varied considerably, according to the variety and the locus examined. No significant correlation was found between SSR and morphological data. However, genetic distances measured by SSRs were correlated to pedigree. These results suggested that SSRs could be used for pre-screening or grouping of existing and candidate varieties, allowing the number of varieties that need to be grown for comparison to be reduced. Multiplex SSR sets gave high-throughput reproducible results, thus reducing the costs of SSR assessment. Multiplex SSR sets are a promising way forward for complementing the current variety testing system in B. napus.

show abstract

Microsatellite markers for genome analysis in Brassica. II. Assignment of rapeseed microsatellites to the A and C genomes and genetic mapping in Brassica oleracea L.

Cited by 53 publications

References 17 publications

Screening and Estimation of Allelic Differentiation in Indian mustard Using SSR Markers for Background Selection

Screening and Estimation of Allelic Differentiation in Indian mustard Using SSR Markers for Background Selection

Efficient large-scale development of microsatellites for marker and mapping applications in Brassica crop species

The development of multiplex simple sequence repeat (SSR) markers to complement distinctness, uniformity and stability testing of rape (Brassica napus L.) varieties

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