Microscopic, phytochemical, HPTLC, GC–MS and NIRS methods to differentiate herbal adulterants: Pepper and papaya seeds

Vadivel, Vellingiri; Natesan, Ravichandran; Rajalakshmi, Perumal; Brindha, P.; Gopal, A.; Kumaravelu, C.

doi:10.1016/j.hermed.2018.01.004

Cited by 37 publications

(19 citation statements)

References 19 publications

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“…Herbal medicines are prepared using different plant sources either in its original form or by after processing it with different constituents. In the traditional Indian system of medicine, there are number of herbal remedies having medicinal values but are not emerged in worldwide market due to lack of validated quality control procedures (Vadivel et al 2018). This is done to compare the various factors at a glance (Rajakrishnan et al 2016).…”

Section: Discussionmentioning

confidence: 99%

Antidiabetic activity of standardized dried tubers extract of Aconitum napellus in streptozotocin‐induced diabetic rats

et al. 2020

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India has got rich cultural inheritage in the forms of Ayurveda texts which are a rich and ample source of herbs, shrubs, trees and affluent in medicinally active phytoconstituents. Aconitum napellus is used for the cure of many ailments including rheumatoid arthritis, sciatica and gout. The present work attempts to evaluate the physicochemical and preliminary phytochemical studies on the tubers of Aconitum napellus along with its antidiabetic activity. The herbal standardization was carried out on the basis of organoleptic properties, physical characteristics and physicochemical properties. The body weight of ACON-I (1.25 mg/kg) and ACON-II (2.5 mg/kg) was recorded as 190.40 and 209.40 g, respectively, compared with 163.00 g in diabetic rats at day 28. The body weight of ACON-I and ACON-II was significantly increased compared with diabetic rats (p < 0.01). However, the body weight of ACON-I and ACON-II was decreased significantly (p < 0.01) compared with normal group (222.60 g). The blood glucose levels of diabetic rats and ACON-I group were recorded as 277.800 and 152.400 mg/dl, respectively, compared with 83.600 mg/dl in normal rats (p < 0.01). However, the HbA1c levels of diabetic rats and ACON-I group were recorded as 11.306 and 6.936% Hb, respectively, compared with 4.539% Hb in normal rats. The glucose and HbA1c levels of diabetic and ACON-I groups were significant compared with normal group (p < 0.01). The results of antidiabetic activity showed that the plant can be used as a potent source for the treatment of diabetes and its complications. The results of this work provided the referential information for the identification and standardization of Aconitum napellus along with its role as a hypoglycemic agent.

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Section: Discussionmentioning

confidence: 99%

Antidiabetic activity of standardized dried tubers extract of Aconitum napellus in streptozotocin‐induced diabetic rats

et al. 2020

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“…posed the detection of secondary metabolites by classical phytochemical tests when defining quality standards for medicinal plant species [65][66][67][68][69][70][71][72][73][74] (Table 2S, Supporting Information). Among the reviewed phytochemical screening studies, the most frequently used tests were those aimed at detecting the presence of flavonoids and other polyphenols, tannins, alkaloids, saponins, steroids, and triterpenoids.…”

Section: Phytochemical Screeningmentioning

confidence: 99%

Quality Control of Herbal Medicines: From Traditional Techniques to State-of-the-art Approaches

et al. 2021

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Herbal medicines are important options for the treatment of several illnesses. Although their therapeutic applicability has been demonstrated throughout history, several concerns about their safety and efficacy are raised regularly. Quality control of articles of botanical origin, including plant materials, plant extracts, and herbal medicines, remains a challenge. Traditionally, qualitative (e.g., identification and chromatographic profile) and quantitative (e.g., content analyses) markers are applied for this purpose. The compound-oriented approach may stand alone in some cases (e.g., atropine in Atropa belladonna). However, for most plant materials, plant extracts, and herbal medicines, it is not possible to assure quality based only on the content or presence/absence of one (sometimes randomly selected) compound. In this sense, pattern-oriented approaches have been extensively studied, introducing the use of multivariate data analysis on chromatographic/spectroscopic fingerprints. The use of genetic methods for plant material/plant extract authentication has also been proposed. In this study, traditional approaches are reviewed, although the focus is on the applicability of fingerprints for quality control, highlighting the most used approaches, as well as demonstrating their usefulness. The literature review shows that a pattern-oriented approach may be successfully applied to the quality assessment of articles of botanical origin, while also providing directions for a compound-oriented approach and a rational marker selection. These observations indicate that it may be worth considering to include fingerprints and their data analysis in the regulatory framework for herbal medicines concerning quality control since this is the foundation of the holistic view that these complex products demand.

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“…The Phytochemical constituents were recognized by associating the results of the mass spectrum with the inbuilt NIST library database. [19][20][21][22] In vitro antioxidant activity…”

Section: Gc-ms Analysismentioning

confidence: 99%

Preliminary Phytochemical Studies, GC-MS Analysis and In vitro Antioxidant Activity of Selected Medicinal Plants and its Polyherbal Formulation

Shalini¹,

Ilango²

2021

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Background: Novel polyherbal formulation (PHF) is the utilization of more than one herb in the preparation of herbal medication. The thought is found in the conventional system of medicine where the variety of herbs in a specific proportion of illness. Because of synergism, polyherbalism presents a few advantages which aren't accessible in single herbal medication. It is utilized in these medications for the treatment of numerous sicknesses including antioxidants. Objective: To develop a phytochemical screening and GC-MS analysis of Novel Polyherbal formulation for In vitro antioxidant activity. Materials and Methods: Macroscopical, preliminary phytochemical, quantitative phytoconstituents, and In-vitro antioxidant activity of all the individual extract and polyherbal formulation was done by chemical method. Identification of phytoconstituents with the aid of Gas chromatography -Mass spectroscopy (GC-MS). Results: Macroscopical study and physicochemical examination, for example, ash value, extractive value, loss on drying, and pH were reported to A. racemosus, B. variegata, C. bonducella, S. asoka, and S. racemosus and novel polyherbal formulation. Qualitative phytochemical investigation revealed the presence of alkaloids, flavonoids, gums & mucilage, carbohydrates, steroids, proteins & amino acids, fats & fixed oils, glycoside, phenols, and saponins. Quantitative estimation such as TAC, TFC, TGC, TSC, and TPC was showed positive results. All the individual extract and PHF were subjected to GC-MS analysis. All the individual extract and polyherbal formulation displayed strong antioxidant activity. Conclusions: To conclude the PHF was reported that high level of bioactive contents present and strong antioxidant activity in contrast to the preferred ascorbic acid. The GC-MS uncovered the presence of bioactive compounds and these compounds are suggested to treat antibacterial, antioxidant, anti-inflammatory, and antiviral, anti-tumor, anti-proliferative activity, and antifungal activity.

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Microscopic, phytochemical, HPTLC, GC–MS and NIRS methods to differentiate herbal adulterants: Pepper and papaya seeds

Cited by 37 publications

References 19 publications

Antidiabetic activity of standardized dried tubers extract of Aconitum napellus in streptozotocin‐induced diabetic rats

Antidiabetic activity of standardized dried tubers extract of Aconitum napellus in streptozotocin‐induced diabetic rats

Quality Control of Herbal Medicines: From Traditional Techniques to State-of-the-art Approaches

Preliminary Phytochemical Studies, GC-MS Analysis and In vitro Antioxidant Activity of Selected Medicinal Plants and its Polyherbal Formulation

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