“…6 | SIMULATIONS In order to define the quantitative contribution for each microstructure of wood spruce sample, advanced characterizations of wood response under mechanical loading are needed, for instance defining the behavior of the grown tracheids microstructures direction as the lamellar microstructures with perpendicular directions. Therefore, it is required the in-situ imaging of the arrangement deformation that would properly show the performance evolution of the tested sample.Although direct measurement techniques such as SEM and AFM can be applied to determine the morphology changes of several microstructures(Ghisleni et al, 2009;Kawasaki et al, 2017;Zhu, Zhang, & Zhao, 2016), they are commonly hard to perform and it takes a lot of time. Furthermore, the continuous irregular ordering of the microstructure throughout the real sample make simulation more practical; since simulations methods do not require any additional experiments to iterate the mechanical properties or boundary conditions(Samak, Fischer, & Rittel, 2007;Yuan, Lee, & Guilkey, 2010).Then, finite element (FE) simulations of wood microstructure under compression and tension tests were carried out using ANSYS Academic Research Mechanical (Release 18.1), ANSYS Inc. A two-dimensional (2D) symmetric model was used in order to reconstruct, as much as possible, the true wood microstructure constituted of growth tracheids asFIGURE 2 AFM imaging taken in the transverse stem section for (a) a earlywood fiber and (b) a latewood fiber.…”