Microwave Assisted Rapid Diagnosis of Plant Virus Diseases by Transmission Electron Microscopy

Zechmann, Bernd; Graggaber, Gerhard; Zellnig, Günther

doi:10.3791/2950

Cited by 3 publications

(2 citation statements)

References 15 publications

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“…Previous studies to investigate G. duodenalis used morphological methods or molecular technologies based on one or two gene loci (Table 1 ). Morphological examination is time- and labor-consuming, and cannot identify assemblages [ 26 ]. Molecular assay using one or two gene loci could not differentiate mixed infectious and did not provide sufficient information to understand the possible zoonotic links [ 27 ].…”

Section: Introductionmentioning

confidence: 99%

Prevalence and multilocus genotyping of Giardia duodenalis in pigs of Shaanxi Province, northwestern China

et al. 2017

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BackgroundGiardiasis, caused by Giardia duodenalis (syn. Giardia intestinalis, Giardia lamblia), is a significant zoonotic parasitic disease of animals and humans worldwide. Accurate genotyping of G. duodenalis is essential for efficient control and management of giardiasis. The objectives of the present study were to investigate the prevalence and assemblages of giardiasis in pigs in Shaanxi Province, northwestern China, and for the first time study multilocus genotypes (MLGs) in pigs using multilocus genotyping technology in this region.ResultsOf 560 faecal samples collected from five farms in Shaanxi Province, 45 were positive for G. duodenalis and significant differences in prevalence were observed among different locations. Differences in prevalence were also detected in pigs of different age groups, with the highest prevalence in sows and the lowest in boars. Two assemblages, A and E, were identified, and a mixed infection of both A and E was identified in one faecal sample. Assemblage E was predominant and widely distributed in all investigated areas and age groups. Genetic viability was detected for both assemblages, and four different multi-locus genotypes (MLGs) within assemblage E were found, MLGE1-MLGE4.Conclusions Giardia duodenalis was detected in pigs from Shaanxi Province, northwestern China, and genetic diversity was observed in these infections. Both assemblages A and E were detected, and four distinct MLGs within assemblage E were identified. These findings provide new data for controlling G. duodenalis infection in pigs.

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Section: Introductionmentioning

confidence: 99%

Prevalence and multilocus genotyping of Giardia duodenalis in pigs of Shaanxi Province, northwestern China

et al. 2017

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“…Recently, we have developed protocols for ultrastructural investigations that reduce plant sample preparation time for transmission electron microscopy (TEM) from 4 days to about 2–4 h [ 12 – 14 ]. Reduction in sample preparation time by microwave irradiation can be attributed to dielectric heating.…”

Section: Introductionmentioning

confidence: 99%

Rapid immunohistochemical diagnosis of tobacco mosaic virus disease by microwave-assisted plant sample preparation

2013

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Immunoelectron microscopy is a powerful method to diagnose viral diseases and to study the distribution of the viral agent within plant cells and tissues. Nevertheless, current protocols for the immunological detection of viral diseases with transmission electron microscopy (TEM) in plants take between 3 and 6 days and are therefore not suited for rapid diagnosis of virus diseases in plants. In this study, we describe a method that allows rapid cytohistochemical detection of tobacco mosaic virus (TMV) in leaves of tobacco plants.With the help of microwave irradiation, sample preparation of the leaves was reduced to 90 min. After sample sectioning, virus particles were stained on the sections by immunogold labelling of the viral coat protein, which took 100 min. After investigation with the TEM, a clear visualization of TMV in tobacco cells was achieved altogether in about half a day. Comparison of gold particle density by image analysis revealed that samples prepared with the help of microwave irradiation yielded significantly higher gold particle density as samples prepared conventionally at room temperature.This study clearly demonstrates that microwave-assisted plant sample preparation in combination with cytohistochemical localization of viral coat protein is well suited for rapid diagnosis of plant virus diseases in altogether about half a day by TEM.

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External Quality Assurance (EQA) in Diagnostic Electron Microscopy (DEM) of Infectious Diseases: Aims and Roots, Results and Perspectives

Gelderblom¹,

Møller²,

Laue³

2016

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Microwave Assisted Rapid Diagnosis of Plant Virus Diseases by Transmission Electron Microscopy

Cited by 3 publications

References 15 publications

Prevalence and multilocus genotyping of Giardia duodenalis in pigs of Shaanxi Province, northwestern China

Prevalence and multilocus genotyping of Giardia duodenalis in pigs of Shaanxi Province, northwestern China

Rapid immunohistochemical diagnosis of tobacco mosaic virus disease by microwave-assisted plant sample preparation

External Quality Assurance (EQA) in Diagnostic Electron Microscopy (DEM) of Infectious Diseases: Aims and Roots, Results and Perspectives

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