Microwave-based biosensor for on-chip biological cell analysis

Dubuc, David; Mazouffre, Olivier; Llorens, Clément; Taris, Thierry; Poupot, Mary; Fournié, Jean‐Jacques; Bégueret, Jean-Baptiste; Grenier, Katia

doi:10.1007/s10470-013-0111-1

Cited by 14 publications

(7 citation statements)

References 8 publications

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“…Additionally, transparency of the cell membrane to microwave signals minimizes electroporation and allows interrogation of the cytoplasmic properties, providing complementary information to conventional measurements at radio frequencies. In the past few years, microwave impedance sensors have been implemented by several groups for the detection of biological cells [45][46][47] and proteins [48]. For instance, Nikolic-Jaric's group detected yeast and mammalian cells within microwave frequencies [45].…”

Section: Introductionmentioning

confidence: 99%

“…Blondy's group reported a biosensor design based on microwave impedance measurements to analyze the growth of different types of adherent cells [46]. Using microchip-based dielectric spectroscopy, Grenier's group characterized dielectric properties of different aqueous solutions [49], biological cell suspensions and a population of adherent cells [47,[50][51][52], and related the measured parameters to cell proliferation and pathogenic states [53]. What's more, Moutier's group reported using dielectric spectroscopy to detect bacteria proliferation in their native culture environment at a frequency range of 1--3 GHz [54].…”

Section: Introductionmentioning

confidence: 99%

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Differentiation of live and heat-killed E. coli by microwave impedance spectroscopy

Multari

Palego

et al. 2018

Sensors and Actuators B: Chemical

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The detection of bacteria cells and their viability in food, water and clinical samples is critical to bioscience research and biomedical practice. In this work, we present a microfluidic device encapsulating a coplanar waveguide for differentiation of live and heat-killed E.scherichiacoli cells suspended in culture media using microwave signals over the frequency range of 0.5 GHz-20 GHz. From small populations of ∼15 E. coli cells, both the transmitted (|S21|) and reflected (|S11|) microwave signals show a difference between live and dead populations, with the difference especially significant for |S21| below 10 GHz. Analysis based on an equivalent circuit suggests that the difference is due to a reduction of the cytoplasm conductance and permittivity upon cell death. The electrical measurement is confirmed by off-chip biochemical analysis: the conductivity of cell lysate from heat-killed E. coli is 8.22% lower than that from viable cells. Furthermore, protein diffusivity increases in the cytoplasm of dead cells, suggesting the loss of cytoplasmic compactness. These changes are results of intact cell membrane of live cells acting as a semipermeable barrier, within which ion concentration and macromolecule species are tightly regulated. On the other hand, the cell membrane of dead cells is compromised, allowing ions and molecules to leak out of the cytoplasm. The loss of cytoplasmic content as well as membrane integrity areis measurable by microwave impedance sensors. Since our approach allows detection of bacterial viability in the native growth environment, it is a promising strategy for rapid point-of-care diagnostics of microorganisms as well as sensing biological agents in bioterrorism and food safety threats.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Differentiation of live and heat-killed E. coli by microwave impedance spectroscopy

Multari

Palego

et al. 2018

Sensors and Actuators B: Chemical

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“…There are many examples of devices that are based on silicon or glass substrates operating in the microwave frequency range. This technology mostly appears in LOC microsystems, which can be made on, among other things, quartz [ 9 , 45 , 46 , 47 ], silicon [ 48 ], or glass [ 49 , 50 ] substrates. Due to fact that the material, which acts as a microwave circuits’ substrate, should be characterized by high resistivity, the silicon ones are mostly the high-resistive ones [ 51 , 52 , 53 ].…”

Section: Substrate Materials Of Microfluidic-microwave Devicesmentioning

confidence: 99%

Microfluidic Modules Integrated with Microwave Components—Overview of Applications from the Perspective of Different Manufacturing Technologies

Jasińska

Malecha

2021

Sensors

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The constant increase in the number of microfluidic-microwave devices can be explained by various advantages, such as relatively easy integration of various microwave circuits in the device, which contains microfluidic components. To achieve the aforementioned solutions, four trends of manufacturing appear—manufacturing based on epoxy-glass laminates, polymer materials (mostly common in use are polydimethylsiloxane (PDMS) and polymethyl 2-methylpropenoate (PMMA)), glass/silicon substrates, and Low-Temperature Cofired Ceramics (LTCCs). Additionally, the domains of applications the microwave-microfluidic devices can be divided into three main fields—dielectric heating, microwave-based detection in microfluidic devices, and the reactors for microwave-enhanced chemistry. Such an approach allows heating or delivering the microwave power to the liquid in the microchannels, as well as the detection of its dielectric parameters. This article consists of a literature review of exemplary solutions that are based on the above-mentioned technologies with the possibilities, comparison, and exemplary applications based on each aforementioned technology.

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“…Dielectric spectroscopy was invented by physicist Peter Deby in 1912 as a technique suited for physics [16]. In the past century, it has become a well-established method for biological matter characterization [17] due to the strong dielectric contrast between various bio-species [18]. Variations in water content and physical parameters of a sample produce changes in the dielectric properties of the substance under test, raising the ability to analyze biomaterial without altering its biological state, which is essential for quantitative biology.…”

Section: Introductionmentioning

confidence: 99%

A 4 × 4 Array of Complementary Split-Ring Resonators for Label-Free Dielectric Spectroscopy

et al. 2021

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In this study, complementary split-ring resonator (CSRR) metamaterial structures are proposed for label-free dielectric spectroscopy of liquids in microplates. This novel combination of an array of sensors and microplates is readily scalable and thus offers a great potential for non-invasive, rapid, and label-free dielectric spectroscopy of liquids in large microplate arrays. The proposed array of sensors on a printed circuit board consists of a microstrip line coupled to four CSRRs in cascade with resonant frequencies ranging from 7 to 10 GHz, spaced around 1 GHz. The microwells were manufactured and bonded to the CSRR using polydimethylsiloxane, whose resonant frequency is dependent on a complex relative permittivity of the liquid loaded in the microwell. The individual microstrip lines with CSRRs were interconnected to the measurement equipment using two electronically controllable microwave switches, which enables microwave measurements of the 4 × 4 CSRR array using only a two-port measurement system. The 4 × 4 microwell sensor arrays were calibrated and evaluated using water-ethanol mixtures with different ethanol concentrations. The proposed measurement setup offers comparable results to ones obtained using a dielectric probe, confirming the potential of the planar sensor array for large-scale microplate experiments.

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Microwave-based biosensor for on-chip biological cell analysis

Cited by 14 publications

References 8 publications

Differentiation of live and heat-killed E. coli by microwave impedance spectroscopy

Differentiation of live and heat-killed E. coli by microwave impedance spectroscopy

Microfluidic Modules Integrated with Microwave Components—Overview of Applications from the Perspective of Different Manufacturing Technologies

A 4 × 4 Array of Complementary Split-Ring Resonators for Label-Free Dielectric Spectroscopy

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