Mikrostrukturierung von Oberflächen durch reaktive Polymerbeschichtungen

Abstract

Durch chemische Dampfabscheidung (CVD) wurden verschiedenartige Substrate mit einem hochreaktiven Polymer beschichtet, das aktive Pentafluorphenylestergruppen enthält (siehe Schema). Durch Mikrokontaktdrucken wurden dann Biotin‐modifizierte Mikrostrukturen auf der Substratoberfläche hergestellt, die spezifisch mit Fluorescein‐markiertem Streptavidin wechselwirken und somit auch fluoreszenzmikroskopisch sichtbar gemacht werden können.

Reactive Polymer Coatings that “Click”

“…However, further use is currently hampered by the availability of polymer coatings that can undergo surface‐directed dipolar cycloadditions. In the past, chemical vapor deposition (CVD) polymerization of substituted [2,2]paracyclophanes has been instrumental in creating a wide array of functionalized poly‐( p ‐xylylenes) with a diverse class of functional groups, such as amines,13, 14 esters,15–17 aldehydes,18 and alcohols,19–21 which enable the immobilization of biomolecules.…”

Mechanistic Insights into Photochromic Behavior of a Ruthenium(II)–Pterin Complex

“…Reversal of the reaction sequence is not possible in this specific example. However, the newly synthesized CVD coating with activated alkyne groups could also be used in conjunction with other bio‐orthogonal reactions that have been previously shown to be compatible with CVD coatings 11–14. Ultimately, the “double‐click” approach proposed herein as well as other bio‐orthogonal immobilization strategies3a will likely find applications in areas, in which controlled immobilization of multiple ligands is needed.…”

“…Chemical vapor deposition (CVD) polymerization10 is a versatile coating process that effectively decouples the surface chemistry from the bulk composition. The CVD polymerization of functionalized [2.2]paracyclophanes can result in functionalized poly( p ‐xylylene) coatings with a wide range of different groups including active esters,11 aldehydes,12 ketones,13 or anhydrides 14. These coatings have been used for the immobilization of proteins,15 peptides,13 DNA,16 and cells 17.…”

“…μCP is a commonly used method to generate micro‐scale patterns and has been used in the past to modify functionalized poly( p ‐xylylene)s 8b. 11, 15 By using μCP, high local concentrations of reagents in the contact area can be achieved that can lead to significantly increased reaction rates 29. Rozkiewics et al.…”

Reaktive Polymere, die “klicken”

“…Dabei wurden Poly( p ‐xylylene) mit unterschiedlichen funktionellen Gruppen, z. B. Amine,13, 14 Ester,15–17 Aldehyde18 und Alkohole,19–21 synthetisiert und zur Immobilisierung von Biomolekülen genutzt.…”