Milestones in Plant Tissue Culture Systems for the Production of Secondary Products

Production of the Alkaloids Emetine and Cephaeline in Callus Cultures ofCephaelis ipecacuanha

“…The emetine and cephaeline contents of Ipecac roots collected from the Rongo area have been reported to be 1.28 and 0.53 %, respectively (3). It has been commented that plant cell culture for producing secondary metabolites is necessary only when good farmland is not abundant (4) To our knowledge, there is no report on alkaloid production in tissue culture of Ipecac. The present study was undertaken to establish tissue cultures of C. ipecacuanha and to study the accumulation of emetine and cephaeline in callus cultures.…”

Studies on trigonelline from Moringa oleifera and its in vitro regulation by feeding precursor in cell cultures

“…Exogenous supply of a precursor to culture medium has been advocated to increase the yield of the desired product in cell cultures (Staba, 1985). Successful attempts have been made to increase the production of metabolites by supplying precursor or intermediate compounds in vitro (Moreno et al, 1993;Whitmer et al, 1998;Silvestrini et al, 2002;Namdeo et al, 2007).…”

Production of Alkaloids in Plant Cell Culture

“…Over the last decade the potential of cultured plant cells as a means of producing important secondary metabolites has been a focus of investigation (Staba, 1985;Rokem and Goldberg, 1985;Fowler, 1986;Yamada and Fujita, 1986;Banthorpe, 1994). Plant biotechnology may be described (Constabel, 1990) as the enhancement of the formation and accumulation of desirable natural products as well as possible product modification in plants.…”