Production of the Alkaloids Emetine and Cephaeline in Callus Cultures ofCephaelis ipecacuanha

Abstract

Callus cultures were established from hypocotyl explants of Cephaelis ipecacuanha; emetine and cephaeline were identified by HPLC in callus cultures. The effect of macrosalts of different basal medium and hormones on growth and alkaloid accumulation in callus tissues was investigated. Best growth in terms of wet/dry weight was obtained on media containing macrosalts of B5 supplemented with 3 % sucrose, 8mg l_' IBA, 4mg l' IAA, and 4mg 1_i NAA. The highest yields of alkaloids were detected in medium containing macrosalts of SH with same hormone combination.

Effect Of Some Plant Extracts and Nicotinamide on Certain Histopathological Aspects In The Red Palm Weevil Larvae, Rhynchophorus Ferrugineus (Oliver) (Coleoptera: Curculionidae)

“…In our phytochemical studies, various active fractions were extracted from two different acetonic gum extracts of Carapichea ipecacuanha and Eucalyptus camaldulensis. Piperine and rhodopin were extracted from Carapichea ipecacuanha, this is comparable to the study of Jha et al [17] and Yoshimatsu and Shimomura [18] who extracted several alkaloid derivatives of cephaeline and emetine. Gallic acid and lucenin-2 were isolated from Eucalyptus camaldulensis, similar results were obtained by Miranda et al [19], Shayoub et al [20], Pandey and Singh [21] and Ben Hassine et al [22] all extracted gallic acid and tannin Catechin compound in different ratios from Eucalyptus camaldulensis.…”

Reproductive studies in ipecac (Psychotria ipecacuanha (Brot.) stockes; Rubiaceae): pollen development and morphology

“…The native populations are restricted to three regions: Central America, Southwest of the Brazilian Amazon region (Mato Grosso and Rondonia States) and Atlantic forest, mainly in Minas Gerais, Espírito Santo, Rio de Janeiro and Bahia States (Assis, 1992). For the commercial cultivation, ipecac may be propagated by the sexual and asexual methods (Jha et al, 1988). The natural propagation occurs by the both vegetative multiplication and seeds that are transported by the birds, but the former is more important (Sick, 1993).…”

Production of Medicinal Compounds from Endangered and Commercially Important Medicinal Plants through Cell and Tissue Culture Technology for Herbal Industry

“…Zenk [4] successfully established cell lines of different plants capable of producing high yields of secondary compounds in cell suspension cultures. The production of solasodine from calli of Solanum elaeagnifolium and cephaeline and emetine from callus cultures of Cephaelis ipecacuanha were successfully achieved [5]. Some of the notable cell culture methods which have been employed for large scale production of metabolites are production of taxol from cell suspension cultures of Taxus mairei [6]; production of paclitaxel and its related taxanes from different Taxus species; production of berberine through cell suspension culture of Coptis japonica; production of vincristine and vinblastine from Catharanthus roseus [7,8], and production of taxoids from cell suspension cultures of Taxus cuspidate [9] ( Table 1).…”