Milk Production and Reproductive Trait Caused by Loc514211 Gene Mutation in Dairy Cows

Abstract

SNP (rs 42688595) terletak di LOC514211, merupakan gen yang tidak terkarakterisasi dan terletak di kromosom 13 pada Bos taurus, memiliki efek yang signifikan terhadap produksi susu dan kualitas susu yang telah diidentifikasi dari sapi perah Cina dan Inggris menggunakan metode GWAS (Genome Wide Association Study) berdasarkan 60k SNP-Chip dalam penelitian sebelumnya. Tujuan penelitian ini adalah untuk mengkonfirmasi efek dari SNP di LOC514211 pada sifat produksi dalam populasi lain pada sapi perah Chinese Holstein. Empat ratus tujuh puluh sapi perah digunakan dalam penelitian ini, dilakukan ekstraksi genom DNA dari sampel darah, genotyping dilakukan dengan metode PCR-RFLP pada sampel DNA. Analisis statistik yang digunakan adalah ANOVA dengan menggunakan metode General Linear Model (GLM) SAS 9.13 untuk mengidentifikasi asosiasi SNP dengan susu dan sifatsifat reproduksi. Hasil penelitian menunjukkan bahwa SNP pada LOC514211 bersifat polimorfik dalam kelompok sapi perah ini dan terdapat ketidakseimbangan Hukum Hardy-Weinberg, serta memiliki perbedaan yang signifikan antar genotipe dalam parameter produksi susu, kualitas susu dan sifat-sifat reproduksi, yang linier dengan hasil penelitian GWAS sebelumnya yang berbasis SNP-Chips. Kesimpulan dari penelitian ini menunjukkan bahwa SNP pada LOC514211 mempunyai kecenderungan berpotensi sebagai penanda bagi sifat-sifat produksi susu dan reproduksi.

Association of single nucleotide polymorphisms in the fatty acid synthase, LOC514211, and fat mass and obesity-associated genes with milk traits in Indonesian-Holstein dairy cattle

“…Based on the Holstein cattle DNA sequence in GenBank, three primer pairs were applied to amplify fragments of the candidate genes (FASN, LOC514211, and FTO) (Table-1) [8,18]. These candidate genes were selected as markers because they are involved in fat synthesis [6-8]. Because milk fat has a genetic correlation with milk yield and milk protein [22], these candidate genes were also used to assess these traits.…”

“…The products were analyzed using the restriction fragment length polymorphism (RFLP) method and first digested using restriction enzymes (Thermo Scientific, USA) in a 15 μL volume consisting of 4 μL PCR product; 1.5 μL buffer; 0.5 μL MscI for FASN, 0.3 μL TaqI for LOC514211, or 0.3 μL HpyCH4III for FTO; and ddH 2 O to the final volume. They were incubated at 37°C for 2 h for FASN or at 65°C for 3 h for LOC514211 and FTO [8,14,18]. The digested results were examined on a 3% agarose gel (stained with ethidium bromide) after 1 h of electrophoresis (50 V) in ×1 Tris-borate-EDTA buffer.…”

“…The role of LOC514211 has not been definitively determined, but Anggraeni et al . [8] found that this gene was associated with milk fat yield. Therefore, these genes were chosen as candidate genes and were predicted to be associated with milk traits, especially milk fat yield.…”

“…In Indonesian-Holstein cattle, some genetic markers have been studied [15-17], but to date, there have been no reports on the three candidate genes selected for the present study. Moreover, most studies on SNP markers only used individual records of dams without confirmation in the next generation [8,18]. Previous studies on various genetic markers have produced very different results in various populations.…”

Milk Yield and Nutrient Adequacy of Lactating Dairy Cow Fed Based on Tofu Waste, Soybean Hulls and Straw

“…Low levels of milk yield is generally caused by several factors, including genetic [1], environment, lactation periode, months of lactation, days open [2], meeting the needs of feed, feed quality [3] and the availability of feed. On dairy farm, the pattern of feeding quite varied in combination i.e napier grass and concentrate [4], king grass, concentrates (bran, corn meal, coconut, pollard, cassava) and tofu [2], corn silage, grass and tofu [5], napier grass, field grass, corn silage, waste of cassava, soybean and coconut [6], napier grass, concentrate and rice bran [7].…”