miR-101b Regulates Lipid Deposition and Metabolism of Primary Hepatocytes in Teleost Yellow Catfish Pelteobagrus fulvidraco

Chen, Guang-Hui; Zhao, Tao; Wei, Xiaolei; Zhang, Dian-Guang; Zhuo, Mei-Qin; Luo, Zhi

doi:10.3390/genes11080861

Cited by 6 publications

(3 citation statements)

References 41 publications

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“…miR-29a and miR-223 were involved in protein and glucose metabolism during the early stages of refeeding of Arctic Charr [ 55 ]. miR-101b mediated lipid deposition and metabolism in the primary hepatocytes of yellow catfish Pelteobagrus fulvidraco [ 56 ].…”

Section: Feedmentioning

confidence: 99%

Profiling miRNAs of Teleost Fish in Responses to Environmental Stress: A Review

Cao

Zhang

et al. 2023

Biology

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miRNAs are a class of endogenous and evolutionarily conserved noncoding short RNA molecules that post-transcriptionally regulate gene expression through sequence-specific interactions with mRNAs and are capable of controlling gene expression by binding to miRNA targets and interfering with the final protein output. The miRNAs of teleost were firstly reported in zebrafish development, but there are recent studies on the characteristics and functions of miRNAs in fish, especially when compared with mammals. Environmental factors including salinity, oxygen concentration, temperature, feed, pH, environmental chemicals and seawater metal elements may affect the transcriptional and posttranscriptional regulators of miRNAs, contributing to nearly all biological processes. The survival of aquatic fish is constantly challenged by the changes in these environmental factors. Environmental factors can influence miRNA expression, the functions of miRNAs and their target mRNAs. Progress of available information is reported on the environmental effects of the identified miRNAs, miRNA targets and the use of miRNAs in fish.

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Section: Feedmentioning

confidence: 99%

Profiling miRNAs of Teleost Fish in Responses to Environmental Stress: A Review

Cao

Zhang

et al. 2023

Biology

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“…miR-101b inhibitor aggravates FAs, including PA (palmitic acid), or OA (OMEGA-3 fatty acids)-induced lipid accumulation ( Figure 5 and Table S2 ). 160 …”

Section: Non-coding Rna In Fatty Acid Metabolismmentioning

confidence: 99%

Decoding the regulatory roles of non-coding RNAs in cellular metabolism and disease

Zong¹,

Wang²,

Cheng³

et al. 2023

Molecular Therapy

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“…The current study investigates their transcriptional regulation, their responsive mechanisms, and their protein subcellular localization in response to Zn. We used HEK293T as our host cells, because they are reliable model cells for investigating the regulatory mechanisms of the promoters [ 41 ], the gene 3′-UTR [ 42 ], ER stress [ 43 ], and the lipid metabolism [ 44 ] of P. fulvidraco . Our study illustrated the zinc-responsive transcription regulation mechanisms of zip10 , zip13 , and zip14 and provides some views regarding Zip-family-mediated zinc homeostatic control in vertebrates.…”

Section: Introductionmentioning

confidence: 99%

Transcriptional Regulation and Protein Localization of Zip10, Zip13 and Zip14 Transporters of Freshwater Teleost Yellow Catfish Pelteobagrus fulvidraco Following Zn Exposure in a Heterologous HEK293T Model

Liu

Tan

et al. 2022

IJMS

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Zip family proteins are involved in the control of zinc (Zn) ion homeostasis. The present study cloned the promoters and investigated the transcription responses and protein subcellular localizations of three LIV-1 subfamily members (zip10, zip13, and zip14) from common freshwater teleost yellow catfish, Pelteobagrus fulvidraco, using in vitro cultured HEK293T model cells. The 2278 bp, 1917 bp, and 1989 bp sequences of zip10, zip13, and zip14 promoters, respectively, were subcloned into pGL3-Basic plasmid for promoter activity analysis. The pcDNA3.1 plasmid coding EGFP tagged pfZip10, pfZip13, and pfZip14 were generated for subsequent confocal microscope analysis. Several potential transcription factors’ binding sites were predicted within the promoters. In vitro promoter analysis in the HEK293T cells showed that high Zn administration significantly reduced the transcriptional activities of the zip10, zip13, and zip14 promoters. The −2017 bp/−2004 bp MRE in the zip10 promoter, the −360 bp/−345 bp MRE in the zip13 promoter, and the −1457 bp/−1442 bp MRE in the zip14 promoter were functional loci that were involved in the regulation of the three zips. The −606 bp/−594 bp KLF4 binding site in the zip13 promoter was a functional locus responsible for zinc-responsive regulation of zip13. The −1383 bp/−1375 bp STAT3 binding site in the zip14 promoter was a functional locus responsible for zinc-responsive regulation of zip14. Moreover, confocal microscope analysis indicated that zinc incubation significantly reduced the fluorescence intensity of pfZip10-EGFP and pfZip14-EGFP but had no significant influence on pfZip13-EGFP fluorescence intensity. Further investigation found that pfZip10 localizes on cell membranes, pfZip14 colocalized with both cell membranes and lysosome, and pfZip13 colocalized with intracellular ER and Golgi. Our research illustrated the transcription regulation of zip10, zip13, and zip14 from P. fulvidraco under zinc administration, which provided a reference value for the mechanisms involved in Zip-family-mediated control of zinc homeostasis in vertebrates.

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miR-101b Regulates Lipid Deposition and Metabolism of Primary Hepatocytes in Teleost Yellow Catfish Pelteobagrus fulvidraco

Cited by 6 publications

References 41 publications

Profiling miRNAs of Teleost Fish in Responses to Environmental Stress: A Review

Profiling miRNAs of Teleost Fish in Responses to Environmental Stress: A Review

Decoding the regulatory roles of non-coding RNAs in cellular metabolism and disease

Transcriptional Regulation and Protein Localization of Zip10, Zip13 and Zip14 Transporters of Freshwater Teleost Yellow Catfish Pelteobagrus fulvidraco Following Zn Exposure in a Heterologous HEK293T Model

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