miR-1285-3p targets TPI1 to regulate the glycolysis metabolism signaling pathway of Tibetan sheep Sertoli cells

Abstract

Glycolysis in Sertoli cells (SCs) can provide energy substrates for the development of spermatogenic cells. Triose phosphate isomerase 1 (TPI1) is one of the key catalytic enzymes involved in glycolysis. However, the biological function of TPI1 in SCs and its role in glycolytic metabolic pathways are poorly understood. On the basis of a previous research, we isolated primary SCs from Tibetan sheep, and overexpressed TPI1 gene to determine its effect on the proliferation, glycolysis, and apoptosis of SCs. Secondly, we investigated the relationship between TPI1 and miR-1285-3p, and whether miR-1285-3p regulates the proliferation and apoptosis of SCs, and participates in glycolysis by targeting TPI1. Results showed that overexpression of TPI1 increased the proliferation rate and decreased apoptosis of SCs. In addition, overexpression of TPI1 altered glycolysis and metabolism signaling pathways and significantly increased amount of the final product lactic acid. Further analysis showed that miR-1285-3p inhibited TPI1 by directly targeting its 3’untranslated region. Overexpression of miR-1285-3p suppressed the proliferation of SCs, and this effect was partially reversed by restoration of TPI1 expression. In summary, this study shows that the miR-1285-3p/TPI1 axis regulates glycolysis in SCs. These findings add to our understanding on the regulation of spermatogenesis in sheep and other mammals.

IMF deposition ceRNA network analysis and functional study of HIF1a in yak

“…Most current research on miR-1285 concentrates on cancers, such as lung squamous cell carcinoma ( 35 ) and thyroid tumors ( 36 ). It has been found that TPI1, a pivotal enzyme in the glycolysis pathway, is a functional target of miR-1285-3p, and miR-1285-3p can diminish TPI1 expression in Sertoli cells ( 37 ). Another study established that inhibiting miR-122 safeguards hepatocytes from lipid metabolic disorders such as NAFLD and curbs lipogenesis by elevating Sirt1 and activating the AMPK pathway ( 38 ).…”

“…Furthermore, we identified several potential regulatory miRNAs (miR-1285-z, miR-122-y, miR-150-x, and miR-130-x). The principal of miR-1285-3p, and can diminish TPI1 expression in Sertoli cells (37). Another study established that inhibiting miR-122 safeguards hepatocytes from lipid metabolic disorders such as NAFLD and curbs lipogenesis by elevating Sirt1 and activating the AMPK pathway (38).…”

The emerging role of exosomes in the development of testicular

“… 89 In addition, abnormalities in germ cell development at any stage can cause impaired spermatogenesis and ultimately lead to reduced fertility or infertility in mice. These studies found that Sertoli cells have an important role in spermatogenesis 90 , 91 and that alterations in cellular connections, cellular communication, and the microenvironment on which spermatogenesis depends can lead to abnormal germ cell development 92 and ultimately to fertility disorders. 93 These findings have provided ideas to address the infertility disorders of oligospermia and weak spermatozoa.…”