miR-1306 induces cell apoptosis by targeting BMPR1B gene in the ovine granulosa cells

Abdurahman, Anwar; Aierken, Wusimanjiang; Zhang, Fei; Obulkasim, Rahmantay; Aniwashi, Jueken; Sulayman, Ablat

doi:10.3389/fgene.2022.989912

Cited by 2 publications

(2 citation statements)

References 48 publications

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“…Some studies have pointed out that the FecB gene mutation increased sheep fecundity due to the inhibition of the BMP/SMAD pathway [ 70 , 71 ]. Interestingly, the two genes we screened ( TGIF1 and TMEFF2 ) compensated for the effect of the FecB mutation in different periods.…”

Section: Discussionmentioning

confidence: 99%

Key mRNAs and lncRNAs of pituitary that affect the reproduction of FecB + + small tail han sheep

Yang,

Tang,

et al. 2024

BMC Genomics

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Background The pituitary directly regulates the reproductive process through follicle-stimulating hormone (FSH) and luteinizing hormone (LH). Transcriptomic research on the pituitaries of ewes with different FecB (fecundity Booroola) genotypes has shown that some key genes and lncRNAs play an important role in pituitary function and sheep fecundity. Our previous study found that ewes with FecB + + genotypes (without FecB mutation) still had individuals with more than one offspring per birth. It is hoped to analyze this phenomenon from the perspective of the pituitary transcriptome. Results The 12 Small Tail Han Sheep were equally divided into polytocous sheep in the follicular phase (PF), polytocous sheep in the luteal phase (PL), monotocous sheep in the follicular phase (MF), and monotocous sheep in the luteal phase (ML). Pituitary tissues were collected after estrus synchronous treatment for transcriptomic analysis. A total of 384 differentially expressed genes (DEGs) (182 in PF vs. MF and 202 in PL vs. ML) and 844 differentially expressed lncRNAs (DELs) (427 in PF vs. MF and 417 in PL vs. ML) were obtained from the polytocous-monotocous comparison groups in the two phases. Functional enrichment analysis showed that the DEGs in the two phases were enriched in signaling pathways known to play an important role in sheep fecundity, such as calcium ion binding and cAMP signaling pathways. A total of 1322 target relationship pairs (551 pairs in PF vs. MF and 771 pairs in PL vs. ML) were obtained for the target genes prediction of DELs, of which 29 DEL-DEG target relationship pairs (nine pairs in PF vs. MF and twenty pairs in PL vs. ML). In addition, the competing endogenous RNA (ceRNA) networks were constructed to explore the regulatory relationships of DEGs, and some important regulatory relationship pairs were obtained. Conclusion According to the analysis results, we hypothesized that the pituitary first receives steroid hormone signals from the ovary and uterus and that VAV3 (Vav Guanine Nucleotide Exchange Factor 3), GABRG1 (Gamma-Aminobutyric Acid A Receptor, Gamma 1), and FNDC1 (Fibronectin Type III Domain Containing 1) played an important role in this process. Subsequently, the reproductive process was regulated by gonadotropins, and IGFBP1 (Insulin-like Growth Factor Binding Protein 1) was directly involved in this process, ultimately affecting litter size. In addition, TGIF1 (Transforming Growth Factor-Beta-Induced Factor 1) and TMEFF2 (Transmembrane Protein With EGF Like And Two Follistatin Like Domains 2) compensated for the effect of the FecB mutation and function by acting on TGF-β/SMAD signaling pathway, an important pathway for sheep reproduction. These results provided a reference for understanding the mechanism of multiple births in Small Tail Han Sheep without FecB mutation.

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Section: Discussionmentioning

confidence: 99%

Key mRNAs and lncRNAs of pituitary that affect the reproduction of FecB + + small tail han sheep

Yang,

Tang,

et al. 2024

BMC Genomics

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“…MiRNAs have emerged as critical post-transcriptional regulators that fine-tune gene expression and are implicated in a myriad of cellular processes, including those pivotal to reproductive biology [ 4 ]. Specifically, the crucial role of miRNAs in regulating GC function and ovarian physiology has been widely recognized [ 5 ]. In GCs, miRNAs exert their regulatory effects at the post-transcriptional level by specifically binding to target mRNAs, thereby affecting their stability or translation efficiency and regulating gene expression [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

MiR-134-3p targets HMOX1 to inhibit ferroptosis in granulosa cells of sheep follicles

Abudureyimu,

Wu,

Chen

et al. 2024

J Ovarian Res

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Background The intricate interplay of gene expression within ovarian granulosa cells (GCs) is not fully understood. This study aimed to investigate the miRNA regulatory mechanisms of ferroptosis during the process of follicle development in lamb GCs. Methods Employing transcriptome sequencing, we compared differentially expressed mRNAs (DE-mRNAs) and miRNAs (DE-miRNAs) in GCs from lambs treated with follicle-stimulating hormone (FL) to untreated controls (CL). We further screened differentially expressed ferroptosis-related genes and identified potential miRNA regulatory factors. The expression patterns of HMOX1 and miRNAs in GCs were validated using qRT‒PCR and Western blotting. Additionally, we investigated the regulatory effect of oar-miR-134-3p on HMOX1 and its function in ferroptosis through cell transfection and erastin treatment. Results We identified a total of 4,184 DE-mRNAs and 304 DE-miRNAs. The DE-mRNAs were mainly enriched in ferroptosis, insulin resistance, and the cell cycle. Specifically, we focused on the differential expression of ferroptosis-related genes. Notably, the ferroptosis-related genes HMOX1 and SLC3A2, modulated by DE-miRNAs, were markedly suppressed in FLs. Experimental validation revealed that HMOX1 was significantly downregulated in FL and large follicles, while oar-miR-134-3p was significantly upregulated compared to that in the CLs. HMOX1 expression was regulated by the targeting effect of oar-miR-134-3p. Functional assays further revealed that modulation of oar-miR-134-3p influenced HMOX1 expression and altered cellular responses to ferroptosis induction by erastin. Conclusion This study suggested that oar-miR-134-3p and HMOX1 may be one of the pathways regulating ferroptosis in GCs. This finding provides new clues to understanding the development and regulatory process of follicles.

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miR-1306 induces cell apoptosis by targeting BMPR1B gene in the ovine granulosa cells

Cited by 2 publications

References 48 publications

Key mRNAs and lncRNAs of pituitary that affect the reproduction of FecB + + small tail han sheep

Key mRNAs and lncRNAs of pituitary that affect the reproduction of FecB + + small tail han sheep

MiR-134-3p targets HMOX1 to inhibit ferroptosis in granulosa cells of sheep follicles

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