miR-130b is an EMT-related microRNA that targets DICER1 for aggression in endometrial cancer

Abstract

Endometrial cancer (EC) is the most common gynecologic malignancy, but the molecular events involved in the development and progression of EC remain unclear. Certain microRNAs (miRNAs) and DICER1 play important roles in cell motility and survival. This study investigated the role of miR-130b and DICER1 in EC. We profiled miR-130b and DICER1 expression in clinical samples explored its relationship with clinical parameters. A luciferase reporter assay assessed the miR-130b targeting potential of DICER1. We show both in vitro and in vivo that miR-130b overexpression along with DICER1 dysfunction leads to tumor aggression and miRNA synthesis abnormalities that are related to cancer hallmarks through DICER1-miRNAs axis modulation. We also identify the mechanism related to this potential tumor predisposing phenotype: miR-130b and loss of DICER1 induced abnormal expression of EMT-related genes, which constitutes a loop regulation of the miR-130b-DICER1-EMT axis.

miR-130b, an onco-miRNA in bladder cancer, is directly regulated by NF-κB and sustains NF-κB activation by decreasing Cylindromatosis expression

“…For example, in colorectal cancer cell lines, miR-130b directly targeted peroxisome proliferator-activated receptor γ (PPARγ), which regulates some key mediators of cell proliferation, such as P21, cyclin A, and PTEN, thus increasing cell proliferation [27]. In another study involving endometrial cancer cell lines, miR-130b suppressed DICER1 expression and led to the deregulation of miR-200, together with some other EMT-related proteins [38]. In the present study, we showed that miR-130b was involved in cell cycle control and the EMT process and, therefore, promoted cell proliferation, as well as the invasion and migration of bladder cancer cells.…”

Mutant p53 (p53-R248Q) functions as an oncogene in promoting endometrial cancer by up-regulating REGγ

“…Wound-healing assay was performed as described previously [35]. The migration index was defined as the distance traveled by the cell monolayer relative to the gap made by the pipette tip at 0 h.…”

MicroRNAs and head and neck cancer: Reviewing the first decade of research

“…Its role in HNSCC is yet to be elucidated but it is related to epithelialmesenchymal transition (EMT) in endometrial carcinoma [89]. It has also been identified as regulating the tumour suppressor gene RUNX3 in gastric cancer [90].…”