MiR-139-5p/ENAH Affects Progression of Hepatocellular Carcinoma Cells

Zhang, Yueming; Li, Meng; Qiu, Yinghuan; Wu, Yuhua; Chen, Shan; Ni, Bin; Tang, Ding; Deng, Zhouzi; Hu, Zhiqiang

doi:10.1007/s10528-022-10204-9

Cited by 4 publications

(3 citation statements)

References 30 publications

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“…Furthermore, functional experiments showed that miR‐139‐5p overexpression significantly inhibited HCC cell proliferation, migration, and invasion, and also promoted cell apoptosis, whereas miR‐139‐5p downregulation contributed to HCC cell progression. All of these findings suggested that miR‐139‐5p acted as a tumor suppressor in HCC progression, which is consistent with previous studies showing the antioncogenic role of miR‐139‐5p in HCC 39,40 . Additionally, miR‐139‐5p negatively modulated DNASE2 expression in HCC cells.…”

Section: Discussionsupporting

confidence: 91%

“…38 Here, the starBase website predicted that miR-139-5p is downregulated in LIHC samples compared to normal previous studies showing the antioncogenic role of miR-139-5p in HCC. 39,40 Additionally, miR-139-5p negatively modulated DNASE2 expression in HCC cells. Therefore, miR-139-5p may affect HCC progression by targeting DNASE2.…”

Section: Circ_0073228 Binds To Mir-139-5pmentioning

confidence: 98%

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Circ_0073228 serves as a competitive endogenous ribonucleic acid to facilitate proliferation and inhibit apoptosis of hepatocellular carcinoma cells by the miR‐139‐5p/deoxyribonuclease II axis

Bai

et al. 2023

The Journal of Gene Medicine

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Background: Hepatocellular carcinoma (HCC) is a common malignancy and has extremely poor prognosis and outcome. Homo sapiens deoxyribonuclease II (DNASE2) has been reported to participate in HCC progression. Here, the role of DNASE2 in HCC cells and the putative upstream circRNA that mediates DNASE2 expression was investigated. Methods:The expression of RNAs in liver hepatocellular carcinoma (LIHC) samples was analyzed by bioinformatic analysis. The proliferation, apoptosis, migration, invasion, and gene expression in HCC cells were investigated using a Cell Counting Kit -8, colony formation, flow cytometry analysis, wound healing, transwell, western blotting, and a quantitative reverse transcriptase-PCR. The binding relationship among circ_0073228, miR-139-5p and DNASE2 was measured by RNA pulldown and luciferase reporter assays.Results: DNASE2 knockdown inhibited proliferation and promoted apoptosis of HCC cells, whereas DNASE2 overexpression showed the opposite results. miR-139-5p targeted DNASE2 and suppressed its expression. Overexpression of miR-139-5p inhibited malignant phenotypes of HCC cells. RPS23-derived circ_0073228, which bound to miR-139-5p, was found to be upregulated in HCC cells. Inhibition of miR-139-5p or overexpression of DNASE2 counteracted the inhibitory effects of circ_0073228 knockdown on HCC cell progression.Conclusions: circ_0073228 serves as an oncogene to facilitate growth and inhibit apoptosis of HCC cells by regulating the miR-139-5p/DNASE2 axis.

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Section: Discussionsupporting

confidence: 91%

Section: Circ_0073228 Binds To Mir-139-5pmentioning

confidence: 98%

Circ_0073228 serves as a competitive endogenous ribonucleic acid to facilitate proliferation and inhibit apoptosis of hepatocellular carcinoma cells by the miR‐139‐5p/deoxyribonuclease II axis

Bai

et al. 2023

The Journal of Gene Medicine

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“…Most of the miRNAs participating in signatures have been revealed to be associated with cancer progression. In miR-139-5p, it suppressed the proliferation and migration of hepatocellular carcinoma cells by downregulating the expression of ENAH ( Zhang et al, 2022 ). Meanwhile, miR-326 can suppresses the Hippo pathway when combined with PAX8 in trophoblast ( Zang et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Construction of a Necroptosis-Related miRNA Signature for Predicting the Prognosis of Patients With Hepatocellular Carcinoma

et al. 2022

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Background: Many miRNAs have been demonstrated to be associated with the prognosis of hepatocellular carcinoma (HCC). However, how to combine necroptosis-related miRNAs to achieve the best predictive effect in estimating HCC patient survival has not been explored.Methods: The mRNA and miRNA expression profile were downloaded from a public database (TCGA-LIHC cohort). Necroptosis-related genes were obtained from previous references, and necroptosis-related miRNAs were identified using Pearson analysis. Subsequently, differential expression miRNAs (DEms) were identified in HCC and paracancer normal samples based on necroptosis-related miRNA expression. The whole set with HCC was randomized into a training set and testing set (1:1). LASSO-Cox regression analysis was used to construct an miRNA signature. Multiple statistical methods were used to validate the clinical benefit of signature in HCC patients, including receiver operator characteristic (ROC) curves, Kaplan–Meier survival analyses, and decision curve analysis (DCA). The downstream target genes of miRNAs were obtained from different online tools, and the potential pathways involved in miRNAs were explored. Finally, we conducted RT-qPCR in SK-HEP-1, THLE-3, and HUH-7 cell lines for miRNAs involved in the signature.Results: The results showed that a total of eight specific necroptosis-related miRNAs were screened between HCC and adjacent tissues in the training set. Subsequently, based on the aforementioned miRNAs, 5-miRNA signature (miR-139-5p, hsa-miR-326, miR-10b-5p, miR-500a-3p, and miR-592) was generated by LASSO-Cox regression analysis. Multivariate Cox regression analysis showed that the risk scores were independent prognostic indicators in each set. The area under curves (AUCs) of 1 year, 3 years, 5 years, and 7 years were high in each set (AUC >0.7). DCA analysis also revealed that the risk score had a potential benefit than other clinical characteristics. Meanwhile, survival analysis showed that the high-risk group showed low survival probabilities. Moreover, the results of enrichment analysis showed that specific miRNAs were mainly enriched in the cAMP signaling pathway and TNF signaling pathway. Finally, the results of RT-qPCR were consistent with the prediction results in public databases.Conclusion: Our study establishes a robust tool based on 5-necroptosis-related miRNAs for the prognostic management of HCC patients.

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MicroRNA-139-5p suppresses non-small cell lung cancer progression by targeting ATAD2

Sun

Liu

2023

Pathology - Research and Practice

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MiR-139-5p/ENAH Affects Progression of Hepatocellular Carcinoma Cells

Cited by 4 publications

References 30 publications

Circ_0073228 serves as a competitive endogenous ribonucleic acid to facilitate proliferation and inhibit apoptosis of hepatocellular carcinoma cells by the miR‐139‐5p/deoxyribonuclease II axis

Circ_0073228 serves as a competitive endogenous ribonucleic acid to facilitate proliferation and inhibit apoptosis of hepatocellular carcinoma cells by the miR‐139‐5p/deoxyribonuclease II axis

Construction of a Necroptosis-Related miRNA Signature for Predicting the Prognosis of Patients With Hepatocellular Carcinoma

MicroRNA-139-5p suppresses non-small cell lung cancer progression by targeting ATAD2

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