miR-148b-3p, as a tumor suppressor, targets son of sevenless homolog 1 to regulate the malignant progression in human osteosarcoma

Liu, Guodong; Mao, Honggang; Liu, Yan; Zhang, Zun; Si, Huaijun; Zhang, Xiaoyan

doi:10.1080/21655979.2022.2031389

Cited by 4 publications

(4 citation statements)

References 33 publications

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“…A large number of reports have unmasked that the expressions of miRNAs are maladjusted in prostate cancer. Referring to existing evidence, miR-148b-3phas been identifiedas the tumor inhibitor in hepatocellular carcinoma (21), gastric cancer as well as osteosarcoma (26).In our research, miR-148b-3pwas revealed tobe sequestered by HOXA11-AS and display a low expression in prostate cancer cells. In addition, miRNAs influence gene expressions by binding to the 3' UTRs of mRNAs (27).…”

Section: Discussionsupporting

confidence: 62%

LncRNA HOXA11-ASmodulates the miR-148b-3p/MLPH axis to promote prostate cancer cell proliferation

Yang¹,

Guan²,

Chen³

et al. 2023

Cell Mol Biol (Noisy-le-grand)

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Section: Discussionsupporting

confidence: 62%

LncRNA HOXA11-ASmodulates the miR-148b-3p/MLPH axis to promote prostate cancer cell proliferation

Yang¹,

Guan²,

Chen³

et al. 2023

Cell Mol Biol (Noisy-le-grand)

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“…Several miRNAs involved in cancer show dysregulated expression in ASD LCLs, including miR-6732-3p , hsa-miR-221-5p [ 64 , 65 ], hsa-miR-21-5p [ 66 , 67 ], hsa-miR-152-3p [ 68 , 69 ], hsa-miR-3529-3p [ 70 , 71 ], and has-miR-148b-3p [ 72 , 73 ]. Additionally, miR-3529-3p was 2-fold upregulated in ASD LCLs and it targets GABRA4 ( Table S7 ) and this miRNA promotes angiogenesis [ 71 ].…”

Section: Discussionmentioning

confidence: 99%

Integrated microRNA–mRNA Expression Profiling Identifies Novel Targets and Networks Associated with Autism

Gill

Dweep

Rose

et al. 2022

JPM

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Autism spectrum disorder (ASD) is a complex neurodevelopmental disorder, with mutations in hundreds of genes contributing to its risk. Herein, we studied lymphoblastoid cell lines (LCLs) from children diagnosed with autistic disorder (n = 10) and controls (n = 7) using RNA and miRNA sequencing profiles. The sequencing analysis identified 1700 genes and 102 miRNAs differentially expressed between the ASD and control LCLs (p ≤ 0.05). The top upregulated genes were GABRA4, AUTS2, and IL27, and the top upregulated miRNAs were hsa-miR-6813-3p, hsa-miR-221-5p, and hsa-miR-21-5p. The RT-qPCR analysis confirmed the sequencing results for randomly selected candidates: AUTS2, FMR1, PTEN, hsa-miR-15a-5p, hsa-miR-92a-3p, and hsa-miR-125b-5p. The functional enrichment analysis showed pathways involved in ASD control proliferation of neuronal cells, cell death of immune cells, epilepsy or neurodevelopmental disorders, WNT and PTEN signaling, apoptosis, and cancer. The integration of mRNA and miRNA sequencing profiles by miRWalk2.0 identified correlated changes in miRNAs and their targets’ expression. The integration analysis found significantly dysregulated miRNA–gene pairs in ASD. Overall, these findings suggest that mRNA and miRNA expression profiles in ASD are greatly altered in LCLs and reveal numerous miRNA–gene interactions that regulate critical pathways involved in the proliferation of neuronal cells, cell death of immune cells, and neuronal development.

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“…Next, experiments were conducted to verify this hypothesis. In contrast to recent publications on miRNAs and target genes in OS, this paper explored the potential signaling pathway mechanism of miR-331-3p/MGAT1 in OS malignancy and provided a reasonable explanation for the experimental phenomenon [ 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

MicroRNA miR-331-3p suppresses osteosarcoma progression via the Bcl-2/Bax and Wnt/β-Catenin signaling pathways and the epithelial-mesenchymal transition by targeting N-acetylglucosaminyltransferase I (MGAT1)

Yang

Xing

et al. 2022

Bioengineered

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Osteosarcoma (OS) is a high-grade malignant disease that is a prevalent primary malignant sarcoma of the bone. The purpose of this investigation was to therefore elucidate the association between miR-331-3p and OS development and to identify a potential underlying mechanism. Key genes involved in OS were selected using GSE65071 dataset from the Gene Expression Omnibus (GEO) database and Gene Expression Profiling Interactive Analysis (GEPIA). Reverse transcription quantitative polymerase chain reaction (RT-qPCR) and Western blotting were conducted to detect miR-331-3p, MGAT1, the epithelial-mesenchymal transition (EMT), Bcl-2/Bax and Wnt/β-Catenin signaling pathways related proteins. Dual-luciferase reporter assay and TargetScan were used for validating interaction between MGAT1 mRNA and miR-331-3p. Biological effects of miR-331-3p and MGAT1 on OS cells were detected employing MTT, Transwell, wound healing and flow cytometry, respectively. MiR-331-3p was under-expressed in OS, and up-regulation or inhibition of its expression could significantly inhibit or promote the malignant phenotypes of OS cells. Furthermore, we found that MGAT1, a target of miR-331-3p, had elevated expression in OS. Interestingly, MGAT1 could partially alleviate the effect of miR-331-3p in vitro . Collectively, miR-331-3p acts as an critical tumor suppressor through inhibiting MGAT1, results in suppressed Wnt/β-Catenin pathway and decreased proliferation of OS cells; leads to increased apoptosis via Bcl-2/Bax pathway and inhibited migration and invasion ability via the EMT.

show abstract

miR-148b-3p, as a tumor suppressor, targets son of sevenless homolog 1 to regulate the malignant progression in human osteosarcoma

Cited by 4 publications

References 33 publications

LncRNA HOXA11-ASmodulates the miR-148b-3p/MLPH axis to promote prostate cancer cell proliferation

LncRNA HOXA11-ASmodulates the miR-148b-3p/MLPH axis to promote prostate cancer cell proliferation

Integrated microRNA–mRNA Expression Profiling Identifies Novel Targets and Networks Associated with Autism

MicroRNA miR-331-3p suppresses osteosarcoma progression via the Bcl-2/Bax and Wnt/β-Catenin signaling pathways and the epithelial-mesenchymal transition by targeting N-acetylglucosaminyltransferase I (MGAT1)

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