miR-185-5p response to usnic acid suppresses proliferation and regulating apoptosis in breast cancer cell by targeting Bcl2

Abstract

Background: Breast cancer is the most common cancer types among women. Recent researches have focused on determining the efficiency of alternative molecules and miRNAs in breast cancer treatment. The aim of this study was to determine the effect of usnic acid response-miR-185-5p on proliferation in the breast cancer cell and to determine its relationship with apoptosis pathway. Methods: The cell proliferation and cell apoptosis rate were significantly increased following the ectopic expression of miR-185-5p in BT-474 cells. Furthermore, the results of cell cycle assay performed by flow cytometry revealed that the transfection with miR-185-5p induced G1/S phase arrest. The apoptosis-related genes expression analysis was performed by qRT-PCR and the direct target of miR-185-5p in BT-474 cells was identified by western blot and luciferase reporter assay. Results: Our data showed that miR-185-5p can cause significant changes in apoptosis-related genes expression levels, suggesting that cell proliferation was suppressed by miR-185-5p via inducing apoptosis in breast cancer cells. According to western blot results, miR-185-5p lead to decrease BCL2 protein level in BT-474 cells and direct target of miR-185-5p was identified as BCL by luciferase reporter assay. Conclusion: This study revealed that miR-185-5p may be an effective agent in the treatment of breast cancer.

LncRNA FAF attenuates hypoxia/ischaemia‐induced pyroptosis via the miR‐185‐5p/PAK2 axis in cardiomyocytes

“…MiR‐185‐5p has been reported to participate in the process of cell death in response to various cell stresses, including hypoxia/ischaemia injury. 27 , 28 , 44 A recent study indicated that inhibition of miR‐185‐5p in endothelial cells contributed to the recovery of heart function after MI. 29 However, the exact mechanism by which miR‐185‐5p regulates cardiomyocyte pyroptosis after hypoxia‐ischaemia remains unknown.…”

“… 25 MiR‐185‐5p was reported to function as a tumour suppressor by inhibiting proliferation and inducing apoptosis in cancers. 26 , 27 , 28 Recently, one study has revealed that downregulation of miR‐185‐5p expression contributes to neovascularization in endothelial cells, which further mitigates the decline in cardiac function in postinfarction mice. 29 Previous studies have suggested that lncRNAs could bind to miRNAs and act as competitive endogenous RNAs (ceRNAs) to regulate cell biological processes, which play a vital role in myocardial infarction.…”

Long non-coding RNA VCAN-AS1 promotes the malignant behaviors of breast cancer by regulating the miR-106a-5p-mediated STAT3/HIF-1α pathway

“…In BC, miRNA also has a significant regulatory effect. For example, miR-185-5p induces a higher level of apoptosis in BC cells by targeting Bcl-2 [ 33 ]. Additionally, miR-615-3p is upregulated in BC and enhances EMT and metastasis of BC by targeting the PICK1/TGFBRI axis [ 34 ].…”

The role of vulpinic acid as a natural compound in the regulation of breast cancer-associated miRNAs

“…Kılıç et al (2019) determined that 67 miRNAs are specifically responsive to usnic acid (lichen secondary metabolite) in MDA-MB-231 cells, while 15 and 8 are specific to BT-474 and MCF-7 cells, respectively [ 27 ]. Değerli et al (2020) identified miR-185-5p as the most promising usnic acid response of miRNAs in BT-474 breast cancer cells [ 39 ]. In this study, we determined that vulpinic acid responsive miRNAs.…”