miR-221 modulates skeletal muscle satellite cells proliferation and differentiation

Abstract

MicroRNAs (miRNAs) are a class of small non-coding RNA molecules, which play important roles in animals by targeting mRNA transcripts for translational repression. Many recent studies have shown that miRNAs are involved in the control of muscle development. In this study, the expression levels of miR-221 in different tissues and during rabbit skeletal muscle satellite cells (SMSCs) differentiation were detected. Gene ontology term enrichment was used to predict the potential biological roles of miR-221. A synthetic miR-221 mimic and a miR-221 inhibitor were used to investigate the functions of miR-221 during SMSCs proliferation and differentiation to further verify the functions of miR-221 in muscle development. In this report, we compared the expression levels of miR-221 in different tissues. The expression levels of miR-221 were upregulated after the induction of differentiation, and then were gradually downregulated during SMSCs differentiation. Overexpression of miR-221 promoted SMSCs proliferation, whereas inhibiting expression restrained proliferation in the EdU and CCK-8 assays. In addition, overexpression of miR-221 led to a decline in the expression levels of the differentiation marker genes MyoG and MHC. miR-221 overexpression suppressed SMSCs myotube formation. On the contrary, inhibition of miR-221 promoted myotube formation. Our data showed that miR-221 increased SMSCs proliferation and decreased differentiation.

miRNA–mRNA network regulation in the skeletal muscle fiber phenotype of chickens revealed by integrated analysis of miRNAome and transcriptome

“…In swine, overexpression of miR-143 could induce the increase of slow fibers through targeting the HDAC4 expression 40 . Also, we found some DEMs identified here that involving muscle cell differentiation and energy metabolisms, such as gga-miR-221, gga-miR-34a and gga-miR-126 [41][42][43] . Moreover, the biological functions of a few miRNAs in chicken muscles was revealed through the GO and pathway analysis of intersection genes.…”

Bone and Muscle Crosstalk in Aging

“…Combined with the negative effect of miR-186 on rat BMSC osteogenesis and the inhibitory action on the final myogenesis differentiation step by influencing myogenin translation, we deduce that miR-186 may be the driving factor in bone-muscle crosstalk during aging (Antoniou et al, 2014;. Furthermore, the increased miR-221 in aged-BMSC may also function in osteosarcopenia, because it was reported that ectopic overexpression of miR-221 in skeletal muscle satellite cells inhibits myotube formation and miR-221 was found to be a negative regulator of bone formation (Zhang et al, 2017;Liu B. et al, 2018;Gan et al, 2020). Xu R. et al (2018) reported aged BMSCs express high levels of miR-31a-5p leading to bias lineage fate of BMSCs to adipocytes through AT-rich sequence binding protein 2 (SATB2), aged BMSCs also release miR-31a-5p enriched EVs to its microenvironment, inducing increase in osteoclast number and function via the RhoA pathway.…”

Expression profiles of circular RNAs in sheep skeletal muscle

“…Previous studies had confirmed that miRNA-29 could target Akt3 to reduce proliferation and facilitate differentiation of myoblasts in skeletal muscle development [ 38 ], and miR-26 inhibits expression of Ezh2, a known suppressor of skeletal muscle cell differentiation [ 39 ]. To our knowledge, overexpression of miR-27a in chronic kidney disease mice attenuated muscle loss, improved grip strength [ 40 ], and miR-221 increased skeletal muscle satellite cells proliferation and decreased differentiation [ 41 ]. miR-133 involved in the formation of myotubes and myoblast proliferation [ 42 , 43 ].…”