miR-582-5p inhibits invasion and migration of salivary adenoid cystic carcinoma cells by targeting FOXC1

Wang, Weiwei; Chen, Bin; Lei, Chengbin; Liu, Guoxin; Wang, Ye-Gang; Chen, Yi; Wang, Youyuan; Zhang, Shanyi

doi:10.1093/jjco/hyx073

Cited by 34 publications

(36 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similarly, miR-582-5p acts as the tumor suppressor by targeting Rab27a in human colorectal carcinoma 22. Recently, miR-582-5p was reported to inhibit invasion and migration of salivary adenoid cystic carcinoma cells by targeting FOXC1 23. In cervical squamous cell carcinoma, mir-582 was reported to be reduced 24.…”

Section: Discussionmentioning

confidence: 99%

Long noncoding RNA UCA1 targets miR-582-5p and contributes to the progression and drug resistance of bladder cancer cells through ATG7-mediated autophagy inhibition

Wu¹,

Ning³

et al. 2019

OTT

View full text Add to dashboard Cite

BackgroundRently, the incidence of bladder cancer has been on the rise. Accumulating researches have been conducted to clarify the molecular mechanisms and potential therapeutic targets of bladder cancer. The present study aims to explore the regulatory mechanism of the urothelial carcinoma-associated 1 (UCA1)-miR-582-5p-ATG7 axis in bladder cancer.MethodsQuantitative real-time polymerase chain reaction was used to detect mRNA level. Relative protein expression was detected by western blot. wound healing assay and transwell were used to determine migration and invasion of cells. in addtion, luciferase reporter assay and immunohistochemistry were performed.ResultsUCA1 expression was upregulated in bladder cancer tissues and cells, while the depletion of UCA1 by shRNA resulted in the suppression of cell proliferation, invasion, migration, and drug resistance. Further studies demonstrated that UCA1 could directly interact with miR-582-5p, and that there was an inverse correlation between miR-582-5p and UCA1. In addition, we found that ATG7 is a target of miR-582-5p and can be downregulated by either miR-582-5p overexpression or UCA1 knockdown. In particular, the autophagy is reduced when UCA1 shRNA is introduced. Moreover, the in vivo experiment further demonstrated the contribution of UCA1 in bladder cancer including tumor growth, invasion, and migration, and UCA1 knockdown can inhibit the aforementioned activities.ConclusionThese results provided evidence for a novel UCA1 interaction regulatory network in bladder cancer, that is, UCA1-miR-582-5p-ATG7-autophagy axis. Our study provides a new insight into the treatment of bladder cancer.

show abstract

Section: Discussionmentioning

confidence: 99%

Long noncoding RNA UCA1 targets miR-582-5p and contributes to the progression and drug resistance of bladder cancer cells through ATG7-mediated autophagy inhibition

Wu¹,

Ning³

et al. 2019

OTT

View full text Add to dashboard Cite

show abstract

“…Further mechanism study showed that miR-582-5p exerted the inhibitory effect through targeting AKT3[22]. Study by Wang et al described that miR-582-5p suppressed metastasis of salivary adenoid cystic carcinoma cells via targeting FOXC1[9]. In gastric cancer, miR-582-5p has been verified to restrain cell growth by down-regulating the expression of AKT3[23].…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies demonstrated that miR-582-5p exerts dual function against different kinds of tumors. For example, Wang et al[9] proved that miR-582-5p inhibits invasion and migration of salivary adenoid cystic carcinoma cells. Study by Zhang et al[10] showed that upregulation of miR-582-5p inhibits cell proliferation, cell cycle progression and invasion in human colorectal carcinoma.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-582-5p suppresses non-small cell lung cancer cells growth and invasion via downregulating NOTCH1

Liu

Deng

et al. 2019

PLoS ONE

View full text Add to dashboard Cite

Non-small cell lung cancer (NSCLC) is the most common cancer worldwide. MicroRNAs have been shown to be correlated with biological processes of various tumors. In this study, we observed that the expression of miR-582-5p was lower in NSCLC tissues than that in para-carcinoma tissues. Ectopic expression of miR-582-5p significantly inhibited NCI-H358 cell proliferation and invasion. Knockdown of miR-582-5p showed the opposite results, with cell growth rate and the invasive capacity of PC-9 cells enhanced. Furthermore, we elucidated that NOTCH1 is a target of miR-582-5p and there is an inverse correlation between miR-582-5p and NOTCH1 expression in NSCLC tissues. Overexpression of NOTCH1 in miR-582-5p-overexpressing NCI-H358 cells could partially reverse the inhibition of cell proliferation and invasion by miR-582-5p. Thus, our research demonstrated that miR-582-5p suppresses NSCLC cell lines’ growth and invasion via targeting oncoprotein NOTCH1 and restoration of miR-582-5p might be feasible therapeutic strategy for NSCLC.

show abstract

“…In thyroid carcinoma, researchers found that the expression of miR-577 was significantly down-regulated in TC tissue and TC-1 cell lines, then up-regulating the expression of miR-577 and targeting the supression of SphK2 could inhibit the proliferation, invasion, and migration abilities of TC-1 cell lines in vitro [9]. In addition, miR-582-5p significantly down-regulated in the salivary adenoid cystic carcinoma and significantly influenced the proliferation, invasion, and migration abilities of salivary adenoid cystic carcinoma cells by targeting FOXC1 [10]. RAB23, a class of Ras-related small GTPase family, was first extracted from the brain, and subsequently the role of RAB23 was reported in multiple types of tumour and was demonstrated to be involved in the development and occurrence of lung cancer, liver cancer, gastric cancer, and breast cancer, as well as being closely related to cancer progression [11,12].…”

Section: Introductionmentioning

confidence: 99%

miR-597-3p inhibits invasion and migration of thyroid carcinoma SW579 cells by targeting RAB23

Wen

Lin

et al. 2021

Endokrynologia Polska

View full text Add to dashboard Cite

show abstract

miR-582-5p inhibits invasion and migration of salivary adenoid cystic carcinoma cells by targeting FOXC1

Abstract: miR-582-5p could suppress effect on the process of invasion and migration in SACC cell lines, and this could occur through its target gene FOXC1.

Cited by 34 publications

References 31 publications

Long noncoding RNA UCA1 targets miR-582-5p and contributes to the progression and drug resistance of bladder cancer cells through ATG7-mediated autophagy inhibition

Long noncoding RNA UCA1 targets miR-582-5p and contributes to the progression and drug resistance of bladder cancer cells through ATG7-mediated autophagy inhibition

MicroRNA-582-5p suppresses non-small cell lung cancer cells growth and invasion via downregulating NOTCH1

miR-597-3p inhibits invasion and migration of thyroid carcinoma SW579 cells by targeting RAB23

Contact Info

Product

Resources

About