miR-613 inhibits cell migration and invasion by downregulating Daam1 in triple-negative breast cancer

Xiong, Hua; Yan, Ting; Zhang, Weijie; Shi, Fangfang; Jiang, Xuesong; Wang, Xiaohua; Li, Shoushan; Chen, Ying; Chen, Cheng; Zhu, Yichao

doi:10.1016/j.cellsig.2018.01.013

Cited by 76 publications

(62 citation statements)

References 29 publications

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“…RhoA is a classic small GTPase that is generally thought to be essential for the formation of stress fibres and invasion of cancer cells. Xiong et al 51 reported that miR‐613 suppressed TNBC cell migration and invasion by targeting DAAM1, a novel indirect regulator of RhoA activation 52‐54 . However, to our surprise, Humphries et al 55 found that downregulation of ARHGAP18 by miR‐200b inhibited metastasis of TNBC by increasing the activation of RhoA, suggesting the tumour suppressive role of RhoA in TNBC.…”

Section: Micrornasmentioning

confidence: 81%

Systematic characterization of non‐coding RNAs in triple‐negative breast cancer

et al. 2020

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Triple‐negative breast cancer (TNBC) is one of the most aggressive subtypes of breast cancer with negativity for oestrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor (HER2). Non‐coding RNAs (ncRNAs) make up most of the transcriptome and are widely present in eukaryotic cells. In recent years, emerging evidence suggests that ncRNAs, mainly microRNAs (miRNAs), long ncRNAs (lncRNAs) and circular RNAs (circRNAs), play prominent roles in the tumorigenesis and development of TNBC, but the functions of most ncRNAs have not been fully described. In this review, we systematically elucidate the general characteristics and biogenesis of miRNAs, lncRNAs and circRNAs, discuss the emerging functions of these ncRNAs in TNBC and present future perspectives in clinical practice.

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Section: Micrornasmentioning

confidence: 81%

Systematic characterization of non‐coding RNAs in triple‐negative breast cancer

et al. 2020

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“…In the present study, UCA1 was proved as a sponge for miR-613 in GBC cells. It was reported that miR-613 mimic could repress Daam1 expression in triple-negative breast cancer, which impeded cell invasion and migration [15]. Yang et al reported that lncRNA HOTAIR enhanced c-met expression via sponging miR-613, which facilitated cell the epithelial-mesenchymal transition in retinoblastoma [29].…”

Section: Discussionmentioning

confidence: 99%

“…MicroRNA-613 (miR-613) was demonstrated to play important role in cancer progression. For instance, miR-613 suppressed tumor progression in triple-negative breast cancer [15], bladder cancer [16], and laryngeal squamous cell cancer [17]. To date, the role of miR-613 in GBC has rarely been reported.…”

Section: Introductionmentioning

confidence: 99%

Knockdown of Long Non-coding RNA UCA1 Represses Gallbladder Cancer Advancement by Regulating SPOCK1 Expression via Sponging miR-613

Zhang

Chen

et al. 2020

Preprint

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OBJECTIVE : Patients with advanced gallbladder cancer (GBC) have a lower 5-year survival rate. Long non-coding RNA urothelial carcinoma associated 1 (UCA1) and miR-613 are involved in the progression of various cancers. This study was to explore the regulatory mechanism between UCA1 and miR-613 in GBC. METHODS: The expression levels of UCA1, miR-613, and SPOCK1 mRNA were detected using qRT-PCR. Cell proliferation, migration, invasion, and apoptosis were determined with MTT, transwell, or flow cytometry assays. The levels of SPOCK1 protein, Bax, cleaved-casp-3, and Bcl-2 were determined by western blot analysis. The relationship between miR-613 and UCA1 or SPOCK1 was verified via dual-luciferase reporter and/or RNA immunoprecipitation (RIP) assays. The role of UCA1 in vivo was confirmed by xenograft assay. RESULTS: UCA1 and SPOCK1 were upregulated while miR-613 was downregulated in GBC tissues and cells. UCA1 silencing blocked tumor growth in vivo, impeded cell proliferation, migration, invasion, and induced cell apoptosis in GBC cells in vitro. Notably, UCA1 acted as a sponge for miR-613, which targeted SPOCK1 in GBC cells. Moreover, miR-613 repressed cell proliferation, migration, invasion, and accelerated cell apoptosis in GBC cells. UCA1 enhancement reversed miR-613 mimic-mediated influence on proliferation, migration, invasion, and apoptosis of GBC cells. UCA1 regulated SPOCK1 expression through miR-613. Furthermore, SPOCK1 elevation overturned UCA1 silencing-mediated the malignant behaviors of GBC cells. CONCLUSION: UCA1 knockdown suppressed GBC progression via downregulating SPOCK1 via sponging miR-613, providing an evidence for UCA1 as a target for GBC treatment.

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“…Furthermore, the miR-181a was involved in TNBC metastasis via elevated expression of Bim [72]. [115,116] miR-373 TXNIP Induces cancer cell EMT and metastasis [117,118] miR-10b HOXD10 Promotes invasion and metastasis [119] miR-21 PTEN Lymph node metastasis [120] miR-17/92 cluster COL4A3, LAMA3, TIMP2/3 Lymph node metastases, promote metastasis [121,122] miR-629-3p LIFR Lung metastases [123] miR-455-3p EI24 Promotes proliferation, invasion, and migration [124] miR-125b APC Promotes proliferation, metastasis, and EMT [125] miR-181a Bim Promotes EMT, migratory, and invasive [72] Oncosuppressor miRNAs miR-200a/b/c PKCα, UBASH3B, XIAP Suppress proliferation, migration, invasion, and metastasis, promote apoptosis [77,126,127] miR-205 Unknown Lymph node metastasis [128] let-7 RAS, c-Myc Block growth and reduce metastasis [129] miR-145 MUC1, Arf6, JAM-A, Fascin Reduce cell motility, invasiveness, and metastasis [130][131][132] miR-206 CORO1C Regulates metastasis [133] miR-30a ROR1 Associates with high histological grade and more lymph node metastasis [134] miR-190a/940 Unknown Prevents metastasis and cell invasion [19,135] miR-33b HMGA2, SALL4, Twist1 Inhibit metastasis [114] miR-146a-5p SOX5 Inhibits proliferation and metastasis [136] miR-150 HMGA2 Inhibits metastasis [137] miR-124 ZEB2 Inhibits invasion and metastasis [138] miR-148a WNT1, NRP1 Suppress metastasis [139] miR-126-3p RGS3 Inhibits proliferation, migration, invasion, and angiogenesis [140] miR-508-3p ZEB1 Inhibits cell invasion and EMT [141] miR-613 Daam1 Inhibits cell migration and invasion [142] miR-519d-3p LIMK1 Suppresses growth and mot...…”

Section: Mirnas In Regulation Of Metastasis In Tnbcmentioning

confidence: 99%

“…MiR-190a and miR-940 were also significantly reduced in TNBC tissues to prevent metastasis and cell invasion [19,135]. Additionally, several miRNAs function as oncosuppressor miRNAs involved in metastasis of TNBC, such as miR-33b [114], miR-146a-5p [136], miR-150 [137], miR-124 [138] and miR-148a [139], miR-126-3p [140], miR-508-3p [141], miR-613 [142], miR-519d-3p [143], miR-26a [144], and miR-130a [145], by targeting HMGA2, SALL4 and Twist1, SRY-box transcription factor 5 (SOX5), HMGA2, ZEB2, wnt family member 1 (WNT1) and neuropilin 1 (NRP1), the regulator of G-protein signaling 3 (RGS3), ZEB1, disheveled-associated activator of morphogenesis 1 (Daam1), LIM domain kinase 1 (LIMK1), metadherin (MTDH), FOSL1, and zonula occludens 1 (ZO-1, also called TJP1), respectively.…”

Section: Mirnas In Regulation Of Metastasis In Tnbcmentioning

confidence: 99%

MicroRNAs Involved in Carcinogenesis, Prognosis, Therapeutic Resistance, and Applications in Human Triple-Negative Breast Cancer

Ding

Xiong

et al. 2019

Cells

125

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Triple-negative breast cancer (TNBC) is the most aggressive, prevalent, and distinct subtype of breast cancer characterized by high recurrence rates and poor clinical prognosis, devoid of both predictive markers and potential therapeutic targets. MicroRNAs (miRNA/miR) are a family of small, endogenous, non-coding, single-stranded regulatory RNAs that bind to the 3′-untranslated region (3′-UTR) complementary sequences and downregulate the translation of target mRNAs as post-transcriptional regulators. Dysregulation miRNAs are involved in broad spectrum cellular processes of TNBC, exerting their function as oncogenes or tumor suppressors depending on their cellular target involved in tumor initiation, promotion, malignant conversion, and metastasis. In this review, we emphasize on masses of miRNAs that act as oncogenes or tumor suppressors involved in epithelial–mesenchymal transition (EMT), maintenance of stemness, tumor invasion and metastasis, cell proliferation, and apoptosis. We also discuss miRNAs as the targets or as the regulators of dysregulation epigenetic modulation in the carcinogenesis process of TNBC. Furthermore, we show that miRNAs used as potential classification, prognostic, chemotherapy and radiotherapy resistance markers in TNBC. Finally, we present the perspective on miRNA therapeutics with mimics or antagonists, and focus on the challenges of miRNA therapy. This study offers an insight into the role of miRNA in pathology progression of TNBC.

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miR-613 inhibits cell migration and invasion by downregulating Daam1 in triple-negative breast cancer

Cited by 76 publications

References 29 publications

Systematic characterization of non‐coding RNAs in triple‐negative breast cancer

Systematic characterization of non‐coding RNAs in triple‐negative breast cancer

Knockdown of Long Non-coding RNA UCA1 Represses Gallbladder Cancer Advancement by Regulating SPOCK1 Expression via Sponging miR-613

MicroRNAs Involved in Carcinogenesis, Prognosis, Therapeutic Resistance, and Applications in Human Triple-Negative Breast Cancer

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