miR-638 Inhibits immature Sertoli cell growth by indirectly inactivating PI3K/AKT pathway via SPAG1 gene

Hu, Pandi; Guan, Kaifeng; Feng, Yue; Ma, Changping; Song, Huibin; Li, Yang; Xia, Xuanyan; Li, Jialian; Li, Fenge

doi:10.1080/15384101.2017.1380130

Cited by 36 publications

(26 citation statements)

References 45 publications

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“…In men with Sertoli cell‐only syndrome, the miR‐574‐5p expression level was significantly lower compared to controls (Abu‐Halima, Backes, et al, ), indicating that sustained lower expression of miR‐574‐5p is pivotal for the progression of infertility. MiR‐638 plays a role in pig spermatogenesis by regulating immature Sertoli cell growth and apoptosis by targeting the SPAG1 gene (Hu et al, ). The miR‐320 family is predicted to target the protocadherin alpha proteins that are expressed in the mammalian testes.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA signature in spermatozoa and seminal plasma of proven fertile men and in testicular tissue of men with obstructive azoospermia

et al. 2019

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MicroRNAs (miRNAs) have recently received a significant amount of attention due to their remarkable influence on post‐transcriptional gene regulation. In this study, we aim to provide a catalogue of miRNAs present in spermatozoa, seminal plasma and testicular tissue. Expression profiles of miRNA in spermatozoa and seminal plasma of 16 proven fertile men and testicular tissue of eight men with morphologically and/or histologically confirmed obstructive azoospermia were determined by microarray and RT‐qPCR in combination with bioinformatics analyses. A total of 123, 156 and 133 miRNAs were consistently detected in spermatozoa, seminal plasma and testicular tissue respectively. Sixty‐four miRNAs were shared across all sample types. Based on miRNAs expression level present in each group, correlation analysis showed moderate‐to‐strong correlations within the spermatozoa and seminal plasma samples and a wider range of correlations within the testicular tissue samples. The target genes of known miRNAs appeared to be involved in a wide range of biological processes related to reproduction, development and differentiation of germ cells. Our results suggest that there is a certain similarity between spermatozoa and seminal plasma for the relative miRNA expression changes with respect to testicular tissue and provide an overview of the miRNAs present in each sample type.

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Section: Discussionmentioning

confidence: 99%

MicroRNA signature in spermatozoa and seminal plasma of proven fertile men and in testicular tissue of men with obstructive azoospermia

et al. 2019

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“…For instance, miR-1285 and miR-762 promote porcine sertoli cells by regulating ring finger protein 4 (RNF4) gene expression [94]. Similarly, miR-638 plays roles in pig spermatogenesis by regulating immature Sertoli cell growth and apoptosis by targeting the expression of sperm-associated antigen 1 (SPAG1) gene [95].…”

Section: The Role Of Mirnas In Male Gametogenesismentioning

confidence: 99%

The Role of MicroRNAs in Mammalian Fertility: From Gametogenesis to Embryo Implantation

Salilew‐Wondim

Gebremedhn

Hoelker

et al. 2020

IJMS

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The genetic codes inscribed during two key developmental processes, namely gametogenesis and embryogenesis, are believed to determine subsequent development and survival of adult life. Once the embryo is formed, its further development mainly depends on its intrinsic characteristics, maternal environment (the endometrial receptivity), and the embryo–maternal interactions established during each phase of development. These developmental processes are under strict genetic regulation that could be manifested temporally and spatially depending on the physiological and developmental status of the cell. MicroRNAs (miRNAs), one of the small non-coding classes of RNAs, approximately 19–22 nucleotides in length, are one of the candidates for post-transcriptional developmental regulators. These tiny non-coding RNAs are expressed in ovarian tissue, granulosa cells, testis, oocytes, follicular fluid, and embryos and are implicated in diverse biological processes such as cell-to-cell communication. Moreover, accumulated evidences have also highlighted that miRNAs can be released into the extracellular environment through different mechanisms facilitating intercellular communication. Therefore, understanding miRNAs mediated regulatory mechanisms during gametogenesis and embryogenesis provides further insights about the molecular mechanisms underlying oocyte/sperm formation, early embryo development, and implantation. Thus, this review highlights the role of miRNAs in mammalian gametogenesis and embryogenesis and summarizes recent findings about miRNA-mediated post-transcriptional regulatory mechanisms occurring during early mammalian development.

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“…h The quantification of PLCβ1 protein. *P < 0.05, **P < 0.01, ***P < 0.001, PLCβ1, phospholipase C beta 1; UTR, Untranslated Region; ST, swine testis; NC, negative control; N.S., nonsignificant processes of animal testis development and spermatogenesis via their regulations of cell proliferation, apoptosis, and differentiation [29][30][31][32].…”

Section: Discussionmentioning

confidence: 99%

Integrated analysis of miRNA and mRNA expression profiles in testes of Duroc and Meishan boars

et al. 2020

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Background MicroRNAs (miRNAs) are small non-coding RNAs playing vital roles in regulating posttranscriptional gene expression. Elucidating the expression regulation of miRNAs underlying pig testis development will contribute to a better understanding of boar fertility and spermatogenesis. Results In this study, miRNA expression profile was investigated in testes of Duroc and Meishan boars at 20, 75, and 270 days of age by high-throughput sequencing. Forty-five differentially expressed miRNAs were identified from testes of Duroc and Meishan boars before and after puberty. Integrated analysis of miRNA and mRNA profiles predicted many miRNA-mRNA pairs. Gene ontology and biological pathway analyses revealed that predicted target genes of ssc-mir-423-5p, ssc-mir-34c, ssc-mir-107, ssc-mir-196b-5p, ssc-mir-92a, ssc-mir-320, ssc-mir-10a-5p, and ssc-mir-181b were involved in sexual reproduction, male gamete generation, and spermatogenesis, and GnRH, Wnt, and MAPK signaling pathway. Four significantly differentially expressed miRNAs and their predicted target genes were validated by quantitative real-time polymerase chain reaction, and phospholipase C beta 1 (PLCβ1) gene was verified to be a target of ssc-mir-423-5p. Conclusions This study provides an insight into the functional roles of miRNAs in testis development and spermatogenesis and offers useful resources for understanding differences in sexual function development caused by the change in miRNAs expression between Duroc and Meishan boars.

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miR-638 Inhibits immature Sertoli cell growth by indirectly inactivating PI3K/AKT pathway via SPAG1 gene

Cited by 36 publications

References 45 publications

MicroRNA signature in spermatozoa and seminal plasma of proven fertile men and in testicular tissue of men with obstructive azoospermia

MicroRNA signature in spermatozoa and seminal plasma of proven fertile men and in testicular tissue of men with obstructive azoospermia

The Role of MicroRNAs in Mammalian Fertility: From Gametogenesis to Embryo Implantation

Integrated analysis of miRNA and mRNA expression profiles in testes of Duroc and Meishan boars

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