MiR-6869-5p Induces M2 Polarization by Regulating PTPRO in Gestational Diabetes Mellitus

Wang, Pingping; Ma, Zhenzhi; Wang, Zengyan; Wang, Ximei; Zhao, Guifeng; Wang, Zengfang

doi:10.1155/2021/6696636

Cited by 11 publications

(5 citation statements)

References 36 publications

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“…Stirm et al (60) reported conflicting results on the expression of let-7g. These authors reported higher expression of let-7g in the screening group, however, no difference was observed in the validation group in whole blood samples of German women with GDM compared to controls in the screening group (60) Other articles included in this review reported differential miRNA expression, yet these miRNAs were identified in single studies only (36,37,43,44,48,50,57,59,69,71,74,76,77,80,81).…”

Section: Qualitative Synthesis Of Studiesmentioning

confidence: 87%

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A Big Role for microRNAs in Gestational Diabetes Mellitus

et al. 2022

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Maternal diabetes is associated with pregnancy complications and poses a serious health risk to both mother and child. Growing evidence suggests that pregnancy complications are more frequent and severe in pregnant women with pregestational type 1 diabetes mellitus (T1DM) and type 2 diabetes mellitus (T2DM) compared to women with gestational diabetes mellitus (GDM). Elucidating the pathophysiological mechanisms that underlie the different types of maternal diabetes may lead to targeted strategies to prevent or reduce pregnancy complications. In recent years, microRNAs (miRNAs), one of the most common epigenetic mechanisms, have emerged as key players in the pathophysiology of pregnancy-related disorders including diabetes. This review aims to provide an update on the status of miRNA profiling in pregnancies complicated by maternal diabetes. Four databases, Pubmed, Web of Science, EBSCOhost, and Scopus were searched to identify studies that profiled miRNAs during maternal diabetes. A total of 1800 articles were identified, of which 53 are included in this review. All studies profiled miRNAs during GDM, with no studies on miRNA profiling during pregestational T1DM and T2DM identified. Studies on GDM were mainly focused on the potential of miRNAs to serve as predictive or diagnostic biomarkers. This review highlights the lack of miRNA profiling in pregnancies complicated by T1DM and T2DM and identifies the need for miRNA profiling in all types of maternal diabetes. Such studies could contribute to our understanding of the mechanisms that link maternal diabetes type with pregnancy complications.

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Section: Qualitative Synthesis Of Studiesmentioning

confidence: 87%

“…Other articles included in this review reported differential miRNA expression, yet these miRNAs were identified in single studies only ( 36 , 37 , 43 , 44 , 48 , 50 , 57 , 59 , 69 , 71 , 74 , 76 , 77 , 80 , 81 ).…”

Section: Qualitative Synthesis Of Studiesmentioning

confidence: 99%

A Big Role for microRNAs in Gestational Diabetes Mellitus

et al. 2022

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“…It is reported that PTPRO plays an important role in tumorigenesis by regulating cell proliferation, migration, invasion and inflammation [ 7 ]. Besides, PTPRO has been proved to be highly expressed in placenta-derived macrophages from patients with gestational diabetes mellitus and could participate in maintaining placental microenvironment balance [ 19 ]. Furthermore, literature has demonstrated that PTPRO expression is upregulated in placental mononuclear cells of patients with PE [ 9 ].…”

Section: Discussionmentioning

confidence: 99%

Protein tyrosine phosphatase receptor type O (PTPRO) knockdown enhances the proliferative, invasive and angiogenic activities of trophoblast cells by suppressing ER resident protein 44 (ERp44) expression in preeclampsia

2021

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Preeclampsia (PE), a pregnancy-specific syndrome, is the primary cause of maternal mortality. This work was designed to investigate the specific functions of PTPRO/ ERp44 in the biological behaviors of trophoblast cells and elucidate the underlying molecular mechanism. Constructed siRNA-PTPRO and ERp44 overexpression plasmids were transfected into HTR-8/SVneo and JEG-3 cells for further functional experiments. Subsequently, the proliferation and invasion of trophoblast cells were identified by performing CCK-8, flow cytometry and transwell assay. In addition, tube formation assay was employed to estimate the angiogenic ability of HUVECs incubated with the conditioned media (CM) of HTR-8/SVneo or JEG-3 cells. Importantly, the interaction between PTPRO and ERp44 was analyzed through Co-IP. In the current investigation, it was discovered that downregulation of PTPRO notably facilitated the proliferation and invasion of trophoblast cells and induced a stronger in vitro angiogenesis. Moreover, PTPRO interacted with ERp44 to regulate ERp44 expression. ERp44 overexpression suppressed the proliferative, invasive and angiogenic activities of trophoblast cells. As a result, functions of PTPRO knockdown in the biological behaviors of trophoblast cells were partially abrogated upon elevation of ERp44. To sum up, this current research systematically evidenced that PTPRO could regulate the biological behaviors of trophoblast cells by modulating ERp44. Findings may contribute to a novel therapeutic strategy for PE.

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“…miR-6869-5p was considerably downregulated in placenta-derived macrophages from GDM patients. It prevents macrophage from targeting PTPRO and promotes macrophage polarization to M2-type cells, thus preventing macrophage proliferation and inflammation, and maintaining the balance of the placental microenvironment [ 76 ]. In addition, one study identified 22 dysregulated exosomal miRNAs in the plasma of pregnant women with GDM and verified that upregulated miR-423-5p and downregulated miR-122-5p, miR-148a-3p, miR-192-5p and miR-99a-5p could be used as early predictors of GDM.…”

Section: Role Of Ncrnas In Gdmmentioning

confidence: 99%

The role of ncRNA regulatory mechanisms in diseases—case on gestational diabetes

Gao,

Ren,

Hao

et al. 2023

Briefings in Bioinformatics

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Non-coding RNAs (ncRNAs) are a class of RNA molecules that do not have the potential to encode proteins. Meanwhile, they can occupy a significant portion of the human genome and participate in gene expression regulation through various mechanisms. Gestational diabetes mellitus (GDM) is a pathologic condition of carbohydrate intolerance that begins or is first detected during pregnancy, making it one of the most common pregnancy complications. Although the exact pathogenesis of GDM remains unclear, several recent studies have shown that ncRNAs play a crucial regulatory role in GDM. Herein, we present a comprehensive review on the multiple mechanisms of ncRNAs in GDM along with their potential role as biomarkers. In addition, we investigate the contribution of deep learning-based models in discovering disease-specific ncRNA biomarkers and elucidate the underlying mechanisms of ncRNA. This might assist community-wide efforts to obtain insights into the regulatory mechanisms of ncRNAs in disease and guide a novel approach for early diagnosis and treatment of disease.

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MiR-6869-5p Induces M2 Polarization by Regulating PTPRO in Gestational Diabetes Mellitus

Cited by 11 publications

References 36 publications

A Big Role for microRNAs in Gestational Diabetes Mellitus

A Big Role for microRNAs in Gestational Diabetes Mellitus

Protein tyrosine phosphatase receptor type O (PTPRO) knockdown enhances the proliferative, invasive and angiogenic activities of trophoblast cells by suppressing ER resident protein 44 (ERp44) expression in preeclampsia

The role of ncRNA regulatory mechanisms in diseases—case on gestational diabetes

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