miRNAs Participate in MS Pathological Processes and Its Therapeutic Response

Wu, Ting; Chen, Guangjie

doi:10.1155/2016/4578230

Cited by 29 publications

(21 citation statements)

References 95 publications

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“…MS is an inflammatory and immune-mediated disease of the brain and spinal cord, which is characterized by an abnormal response of both innate and adaptive immune system [20]. Although the etiology and pathogenesis of MS have not fully identified, migration of autoreactive lymphocytes across the Blood-Brain Barrier (BBB) may be a result of axonal demyelination of neurons [21]. The presence of autoreactive and proinflammatory T cells, including Th1 and Th17 in CNS will result in axonal demyelination, degeneration, and tissue damage [22,23].…”

Section: Discussionmentioning

confidence: 99%

miR-320 and Inflammation Regulation in Experimental Autoimmune Encephalomyelitis Through Interference With Tumor Growth Factor-β Signaling Pathway

et al. 2019

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Background: MicroRNAs are small non-coding RNAs that regulate gene expression and involve in many cellular and physiological mechanisms. Recent studies have revealed that dysregulation of microRNAs might contribute to autoimmune disorders such as Multiple Sclerosis (MS). Based on these findings, we examined the potential role of miR-320 isoforms; miR-320-3p and miR-320-5p, in the context of autoimmune neuroinflammation and pathogenesis of EAE, which is an animal model of MS. Materials and Methods: The expression levels of miR-320-3p and miR-320-5p, and their predicted target genes, TGFBR2 and Smad2, were quantified in the CNS tissue in mice with Experimental Autoimmune Encephalomyelitis (EAE) using RT-PCR method. The expression was also examined in splenocytes macrophages and astrocytes. To examine the interaction of miR-320-3p and miR-320-5p with the 3′-UTR of potential target transcripts, the mimic sequences of both isoforms were transfected into splenocytes and then examined by RT-PCR. Results: The expression of both isoforms of miR-320 significantly increased in different phases of EAE and activated lymphocytes, whereas the levels of their predicted target genes, Smad2 and TGFBR2 decreased in these cells. Obtained data revealed that miR-320-5p level significantly increased in activated macrophages and astrocytes; however, the miR 320-3p level did not show significant changes in these cells after Lipopolysaccharide (LPS) stimulation. The levels of TGFBR2 and Smad2 decreased in transfected splenocytes. Conclusion: Our findings suggest that upregulation of miR-320 isoforms might be involved in the neuroinflammation and pathogenesis of MS through targeting and suppression of TGFBR2 and Smad2, i.e. protective genes in MS.

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Section: Discussionmentioning

confidence: 99%

miR-320 and Inflammation Regulation in Experimental Autoimmune Encephalomyelitis Through Interference With Tumor Growth Factor-β Signaling Pathway

et al. 2019

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“…Furthermore, the induction of miR-146a mediated by Tβ4 can modulate the innate and adaptive immune response (Wu and Chen, 2016). For example, the increased level of miR-146a can suppress IFN-γ-dependent Th1 responses and reduce Th1-mediated lesions by Tregs inhibition of signal transducer and activator of transcription 1 (Lu et al, 2010), and diminish adhesion of T cells to endothelial cells (Wu et al, 2015).…”

Section: Conclusive Remarksmentioning

confidence: 99%

Thymosins in multiple sclerosis and its experimental models: moving from basic to clinical application

Severa

Zhang

Giacomini

et al. 2019

Multiple Sclerosis and Related Disorders

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“…This impairs leukocyte adhesion and transmigrates across blood–brain barrier (BBB) into the CNS (T. Wu & Chen, ). Ets‐1 is a target of miR‐326 which plays an important role in regulating Th17 immune responses and BBB breakdown (Wu & Chen, ).…”

Section: Mir‐326 As a Therapeutic Target For Treatmentmentioning

confidence: 99%

The MicroRNA‐326: Autoimmune diseases, diagnostic biomarker, and therapeutic target

Jadideslam

Ansarin

Sakhinia

et al. 2018

Journal Cellular Physiology

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MicroRNAs (miRNAs) are uniquely regulated in healthy, inflamed, activated, cancerous, or other cells and tissues of a pathological state. Many studies confirm that immune dysregulation and autoimmune diseases with inflammation are correlated with various miRNA expression changes in targeted tissues and cells in innate or adaptive immunity. In this review, we will explain the history and classification of epigenetic changes. Next, we will describe the role of miRNAs changes, especially mir-326 in autoimmunity, autoinflammatory, and other pathological conditions. A systematic search of MEDLINE, Embase, and Cochrane Library was presented for all related studies from 1899 to 2017 with restrictions in the English language. In recent years, researchers have concentrated on mostly those roles of miRNA that are correlated with the inflammatory and anti-inflammatory process. Latest studies have proposed a fundamental pathogenic role in cancers and autoinflammatory diseases. Studies have described the role of microRNAs in autoimmunity and autoinflammatory diseases, cancers, and so on. The miRNA-326 expression plays a significant role in autoimmune and other types of diseases.

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miRNAs Participate in MS Pathological Processes and Its Therapeutic Response

Cited by 29 publications

References 95 publications

miR-320 and Inflammation Regulation in Experimental Autoimmune Encephalomyelitis Through Interference With Tumor Growth Factor-β Signaling Pathway

miR-320 and Inflammation Regulation in Experimental Autoimmune Encephalomyelitis Through Interference With Tumor Growth Factor-β Signaling Pathway

Thymosins in multiple sclerosis and its experimental models: moving from basic to clinical application

The MicroRNA‐326: Autoimmune diseases, diagnostic biomarker, and therapeutic target

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