Mites of the Genus &lt;i&gt;Dermatophagoides&lt;/i&gt; in Dust from the Houses of Asthmatic and Other Allergic Patients in North America: Development of a Radioimmunoassay for Allergen Produced by &lt;i&gt;D. farinae&lt;/i&gt; and/or &lt;i&gt;D. pteronyssinus&lt;/i&gt;

Plaits-Mills, Thomas A.E.; Heymann, Peter W.; Chapman, Martin D.; Smith, Thomas F.; Wilkins, S.R.

doi:10.1159/000233773

Cited by 20 publications

(3 citation statements)

References 9 publications

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“…Figure 1 shows D.pteronyssinus, D. farinae and D. microceras; the content of the mite allergen in the dust samples collected at different locations shows large variations. The follow ing guidelines have been suggested by Platts-Mills et al [7] levels > 10,000 ng P|/g dust (Pi = Der p I) should be regarded as high, >2,000 as significant, <2,000 as low and <400ng as very low. Using the above guidelines, about 40% of the dust samples from Denmark and Austria had a total mite antigenic level in the high and significant range.…”

Section: Resultsmentioning

confidence: 99%

Level of Indoor Allergens in Dust from Homes of Allergic and Non-Allergic Individuals

Schwartz¹,

Lind²,

Løwenstein³

1987

Int Arch Allergy Immunol

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In a number of studies performed on different locations throughout the world and in homes inhabited both by healthy individuals as well as by patients allergic to the specific allergen, the content of the major allergens from the house dust mite (Der p I, m I and f I), cat (Fel d I) and dog (Ag 3, 13) was measured. The comparison between homes of allergies and non-allergies revealed a tendency to high levels of cat allergens in homes of non-allergies. The content of the mite allergens showed large variations both with respect to the total amount of mites and the species of the mites.

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Section: Resultsmentioning

confidence: 99%

Level of Indoor Allergens in Dust from Homes of Allergic and Non-Allergic Individuals

Schwartz¹,

Lind²,

Løwenstein³

1987

Int Arch Allergy Immunol

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“…It has been shown that the allergenic capacity of common house dust mites which frequently cause allergy symptoms (Dermatophagoides pteronyssinus and farinae) is found in their faeces [14]. In order to include the eventual allergens secreted by TU (especially those contained in the cobweb which surrounds their colonies) the TU extract used in the present study was obtained from highly parasitized carnation leaves.…”

Section: Sds-pagementioning

confidence: 99%

Occupational allergy in greenhouse workers: sensitization to Tetranychus urticae

Delgado¹,

Orta²,

Navarro³

et al. 1997

Clin Exp Allergy

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“…In addition, mite allergen measure ments can help to assess the level of exposure to mite allergens [22][23][24][25][26][27][28].…”

Section: Introductionmentioning

confidence: 99%

Measurement of Allergens Associated with Dust Mite Allergy

Yasueda¹,

Mita²,

Y³

et al. 1989

Int Arch Allergy Immunol

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Sensitive radioimmunoassays were developed to measure the two groups of major allergens (Der I and Der II) of Dermatophagoides pteronyssinus and Dermatophagoides farinae, in which radiolabeled protein A served as a general tracer. Glass rods covalently coupled with F(ab′)₂ fragments of rabbit antiserum IgG were incubated first with mite or house dust extracts, and then with affinity-purified rabbit antibodies. The bound allergen-antibody complex was detected with a ¹²⁵I-labeled protein A. The two Der I allergens, Der p I and Der f I, were measured separately with the use of immunoabsorbed rabbit antibodies directed against species-specific determinants, while the two Der II allergens, Der p II and Der f II, were measured as ‘Der II’ with the use of antibodies directed against common determinants on both allergens. Each assay demonstrated consistently parallel dilution curves with mite and house dust extracts. The mean intraassay and interassay coefficient of variation for each assay ranged from 4.3 to 7.7% and from 3.6 to 9.0%, respectively. The Der p I and Der f I assays were shown to be highly species-specific so that the ratio of Der p l:Der f I would provide a good index of the distribution of the two mite species in a dust sample. The concentrations of the Der I and Der II allergens in different types of mite extract and dust samples from houses were compared using these assays. The results demonstrated that the ratio of Der I: Der II in the mite body extracts varied markedly from those in the whole culture extracts, while the ratio in individual dust samples was nearly constant and was close to that in the mite body extracts. These assays will be useful in standardizing mite and house dust extracts and for assessments of mite allergen exposure. Furthermore, the high assay sensitivity will make it possible to use these assays to measure mite allergens associated with airborne particles.

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Mites of the Genus <i>Dermatophagoides</i> in Dust from the Houses of Asthmatic and Other Allergic Patients in North America: Development of a Radioimmunoassay for Allergen Produced by <i>D. farinae</i> and/or <i>D. pteronyssinus</i>

Cited by 20 publications

References 9 publications

Level of Indoor Allergens in Dust from Homes of Allergic and Non-Allergic Individuals

Level of Indoor Allergens in Dust from Homes of Allergic and Non-Allergic Individuals

Occupational allergy in greenhouse workers: sensitization to Tetranychus urticae

Measurement of Allergens Associated with Dust Mite Allergy

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