Mitogenic effects of insulin and insulin‐like growth factors on PA‐III rat prostate adenocarcinoma cells: Characterization of the receptors involved

Polychronakos, Constantin; Janthly, Uma; Lehoux, Jean‐Guy; Koutsilieris, Michael

doi:10.1002/pros.2990190405

Cited by 79 publications

(48 citation statements)

References 30 publications

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“…However, to our knowledge no one has demonstrated a direct effect of insulin on normal prostate tissue. A previous study from our laboratory 18 reported that insulin does stimulate the growth of the human LNCaP PCa cell line, and Polychronakos et al 19 reported that insulin stimulated the growth of rat PA-III adenocarcinoma cells.…”

Section: Discussionmentioning

confidence: 94%

Effect of diet and exercise intervention on the growth of prostate epithelial cells

Barnard

Kobayashi

Aronson

2008

Prostate Cancer Prostatic Dis

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Epidemiological studies suggest a positive association between nutrient intake, hyperinsulinemia and risk of Benign prostatic hyperplasis (BPH). This study tests the hypothesis that a low-fat, highfiber diet and daily exercise would lower serum insulin and reduce the growth of serum-stimulated primary prostate epithelial cells in culture. Serum samples were obtained from eight overweight men before and after the Pritikin residential, 2-week diet and exercise intervention and from seven men who were long-term followers of the low-fat, high-fiber diet and regular exercise lifestyle. The serum was used to stimulate primary prostate epithelial cells in culture. Growth was measured after 48 and 96 h and apoptosis after 96 h. At 48 h there was no significant difference in growth within the Pre, 2-week or Long-Term groups. At 96 h growth was significantly reduced in the 2-week (13%) and in the Long-Term (14%) groups compared to the Pre data. At 96 h, apoptosis was not significantly different among the three groups. Fasting insulin was reduced by 30% in the 2-week group and by 52% in the Long-Term group compared to the Pre data. Testosterone was unchanged in the 2-week group. The results of this study indicate that a low-fat, high-fiber diet and daily exercise lowers insulin and reduces growth of prostate primary epithelial cells and suggests that lifestyle may be an important factor in the development or progression of BPH. Future prospective trials should address the effects of this lifestyle modification on BPH symptomatology and progression.

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Section: Discussionmentioning

confidence: 94%

Effect of diet and exercise intervention on the growth of prostate epithelial cells

Barnard

Kobayashi

Aronson

2008

Prostate Cancer Prostatic Dis

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“…Circulating insulin levels increase linearly with increasing obesity, and insulin has been implicated in prostate cancer biology (50), with higher risk of prostate cancer (51), and with higher recurrence of the disease (52). Circulating concentrations of total IGF-I have been associated with higher risk of prostate cancer (53)(54)(55)(56).…”

Section: Discussionmentioning

confidence: 99%

Body Mass Index, Weight Change, and Risk of Prostate Cancer in the Cancer Prevention Study II Nutrition Cohort

Rodríguez

Freedland

Deka

et al. 2007

Cancer Epidemiology, Biomarkers &Amp; Prevention

318

236

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Background: Obesity has been associated with aggressive prostate cancer. The extent of this association, which varies by stage and grade, remains unclear. The role of recent weight change had not been previously examined. Methods: We examined body mass index (BMI) and weight change in relation to incident prostate cancer by disease stage and grade at diagnosis among 69,991 men in the Cancer Prevention Study II Nutrition Cohort. Participants provided information on height and weight in 1982, and again at enrollment in 1992. During follow-up through June 30, 2003 (excluding the first 2 years of follow-up), we documented 5,252 incident prostate cancers. Cox proportional hazards models were used to estimate rate ratios (RR) and 95% confidence intervals (95% CI).

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“…Isolated epithelial cells of rat ventral prostate have insulin receptors, and fasting increases their concentration (Carmena et al, 1986). But PA-III rat prostate adenocarcinoma cells have no insulin receptor, though they do have specific binding sites for IGF-I and II (Polychronakos et al, 1991).…”

Section: Discussionmentioning

confidence: 99%

Serum insulin level, disease stage, prostate specific antigen (PSA) and Gleason score in prostate cancer

et al. 2002

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In the present study, we assessed the relationship of serum insulin levels and three surrogate markers of recurrence, T stage, PSA, and Gleason score, in men with localized prostate cancer. Participants in our study were found through urology and radiation oncology clinics, and all eligible patients were asked to take part. All patients were asymptomatic and had been initially diagnosed on the basis of rising PSA or abnormal physical examination. Histological confirmation of diagnosis was obtained for all subjects. Serum insulin levels were determined by chemoluminescent assay with a standard, commercially available instrument. Patients were divided into three previously defined risk groups: Low risk : PSA ⩽10, stage ⩽T2a, or Gleason grade ⩽6. Medium risk : 10 7, tumour in seminal vesicle biopsy, PSA >15 or stage T2c or T3. One hundred and sixty-three men with prostate cancer were studied. There was a significant increase in serum insulin with risk group ( P =0.003, one way anova). Tukey's multiple range test showed that the insulin levels of high risk patients were significantly higher than the insulin levels of medium and low risk patients ( P =0.05) but the insulin levels of medium and low risk patients were not significantly different from one another. Multivariate linear regression, with insulin as the dependent variable, Gleason score, PSA, and T stage (T1, T2, T3) as the independent variables, was significant overall ( P <0.001, r 2 =0.120). Increased T stage was independently correlated with increased serum insulin levels ( P <0.001). Gleason score was negatively, insignificantly correlated with serum insulin level ( P =0.059). The positive correlation of PSA and insulin level was not significant ( P =0.097). To assure normal distribution of insulin and PSA values, the regression was repeated with log (insulin) as the dependent variable, log (PSA), T stage (T1, T2, T3), and Gleason score as independent variables. The regression was significant overall ( P =0.002, r 2 =0.095). Increased T stage was independently correlated with increased log (insulin level) ( P =0.026). Gleason score was negatively, insignificantly correlated with log (insulin) level ( P =0.728). The positive correlation of log (PSA) and log (insulin) levels was significant ( P =0.010). The relationship between increased insulin level and advanced tumour stage in prostate cancer we describe here is biologically quite plausible, since insulin is a growth factor. Further studies may document whether serum insulin levels might be a useful biomarker of prostate cancer stage. British Journal of Cancer ...

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Mitogenic effects of insulin and insulin‐like growth factors on PA‐III rat prostate adenocarcinoma cells: Characterization of the receptors involved

Cited by 79 publications

References 30 publications

Effect of diet and exercise intervention on the growth of prostate epithelial cells

Effect of diet and exercise intervention on the growth of prostate epithelial cells

Body Mass Index, Weight Change, and Risk of Prostate Cancer in the Cancer Prevention Study II Nutrition Cohort

Serum insulin level, disease stage, prostate specific antigen (PSA) and Gleason score in prostate cancer

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