Mitospore formation on pure cultures of Tuber japonicum (Tuberaceae, Pezizales) in vitro

Nakano, Shota; Obase, Keisuke; Nakamura, Noritaka; Kinoshita, Akihiko; Kuroda, Katsushi; Yamanaka, Takashi

doi:10.1007/s00572-022-01082-5

Cited by 3 publications

(2 citation statements)

References 30 publications

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“…The formation of mitospores on hyphae surfaces observed in our research constituted the first report of this kind of growth in Morchella ( Figure 2 d–g). Previously, this phenomenon was only recently recorded in another Ascomycete, namely, Tuber japonicum [ 53 ]. The reported homogeneous cell wall, with a medium thickness of 100.75 ± 13.89 nm ( Figure 2 h,i), was much thinner than those reported for hyphae and sclerotia cell walls [ 15 , 54 ].…”

Section: Discussionmentioning

confidence: 98%

Ultrastructure and Physiological Characterization of Morchella Mitospores and Their Relevance in the Understanding of the Morel Life Cycle

Liu

Zhang

et al. 2023

Microorganisms

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Morels, which belong to the Ascomycete genus Morchella, are highly valued edible fungi treasured by gourmet chefs worldwide. Some species are saprotrophic and others are able to form facultative mycorrhizal-like associations with plant roots without establishing true ectomycorrhizal symbioses. In general, it is considered that the formation of asexual spores, or mitospores, is an important step in the life cycle of morels. However, ultrastructure characterization and physiological attributes of morel mitospores have received little attention. In this contribution, the mitospores of M. sextelata were successfully induced under laboratory conditions and their ultrastructure, occurrence, germination, physiological characteristics and mating type gene structure were studied. Mitospore production was closely related to aeration, nutrition and humidity conditions. The average germination rate of mitospores on different media and under various induction stimuli was very low, with an average of 1/100,000. Based on the ultrastructure characterization, low germination rate, growth rate decline, rapid aging and mating genotyping, it was concluded that the mitospores of M. sextelata had lost their conventional function as conidia and might act more as mate sperm-like (gamete) structures. Thus, this study contributed to a deeper understanding of the life cycle of the economically and ecologically important morel fungal group.

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Section: Discussionmentioning

confidence: 98%

Ultrastructure and Physiological Characterization of Morchella Mitospores and Their Relevance in the Understanding of the Morel Life Cycle

Liu

Zhang

et al. 2023

Microorganisms

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“…Similar structures have also been found in the in vitro mycelium of Tuber japonicum Hir. Sasaki, A. Kinosh & Nara [49], but never on T. borchii mycelium in vitro conditions [17].…”

Section: Biologymentioning

confidence: 99%

The Mysteries of the White Truffle: Its Biology, Ecology and Cultivation

Graziosi

Hall²,

Zambonelli

2022

Encyclopedia

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Tuber magnatum Picco is the most expensive of the truffles and a great deal of research has been carried out in an attempt to solve the mysteries of its ecology and biology. However, considerable work remains to be done particularly on those secrets of its life cycle that remain a mystery. It is known that T. magnatum is heterothallic, but it has yet to be determined how fertilization occurs between the two strains of different mating types. It is also known that the white truffle is an ectomycorrhizal fungus, and its mycorrhizas can be produced in greenhouses, but then they seem to disappear in the field. The role of other soil microorganisms, fungi and bacteria, on its soil mycelial development and fructification is intriguing but is far from being completely understood. All these uncertainties have made the cultivation of T. magnatum extremely difficult and only recently have we had the scientific proofs that it is possible. Even so, many questions remain unanswered and the management practices of T. magnatum plantations are still to be better defined to also enable the taming of this truffle.

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Advances in molecular genetics have increased knowledge of Tuber species’ life cycle and population genetic structure, indicating ways to improve yield

Galappaththi,

Dunstan,

Hardy

et al. 2024

Mycorrhiza

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Mitospore formation on pure cultures of Tuber japonicum (Tuberaceae, Pezizales) in vitro

Cited by 3 publications

References 30 publications

Ultrastructure and Physiological Characterization of Morchella Mitospores and Their Relevance in the Understanding of the Morel Life Cycle

Ultrastructure and Physiological Characterization of Morchella Mitospores and Their Relevance in the Understanding of the Morel Life Cycle

The Mysteries of the White Truffle: Its Biology, Ecology and Cultivation

Advances in molecular genetics have increased knowledge of Tuber species’ life cycle and population genetic structure, indicating ways to improve yield

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