Mitoxantrone-loaded BSA nanospheres and chitosan nanospheres for local injection against breast cancer and its lymph node metastases

Lü, Bin; Xiong, Su-Bin; Yang, Hong; Yin, Xiaodong; Zhao, Ruobing

doi:10.1016/j.ijpharm.2005.09.038

Cited by 19 publications

(7 citation statements)

References 7 publications

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“…Although histological sections of Erhlich tumor in the present study had a typical pattern that consisted of a peripheral area of viable tumor layers surrounding a central area of necrotic tissue [37], necrosis areas were found in both the central area and in the peripheral regions of the tumor in the group treated with PDT, where the presence of inflammatory infiltrates and hemorrhages in blood vessels was also observed. Similar results were also reported by Longo et al [25]. Since there was a significant increase in the percentage of necrotic area in the PDT group, the observed infiltration of inflammatory cells after PDT procedure was possibly responsible for the slight reduction in tumor volume when compared to the control tumor.…”

Section: Discussionsupporting

confidence: 90%

“…While photosensitizers can reach the lesion more easily, and thus selectively accumulate in tumor tissues, molecular association with albumin may be performed in physiological solutions, thus conferring biocompatible characteristics to the formulation [20]. To further enhance the selective release and accumulation of photosensitizers at the targeted tissue, various carriers and drug delivery systems have been investigated [1,5,14,21-25]. …”

Section: Introductionmentioning

confidence: 99%

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Antitumor activity of photodynamic therapy performed with nanospheres containing zinc-phthalocyanine

et al. 2013

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BackgroundThe increasing incidence of cancer and the search for more effective therapies with minimal collateral effects have prompted studies to find alternative new treatments. Among these, photodynamic therapy (PDT) has been proposed as a very promising new modality in cancer treatment with the lowest rates of side effects, revealing itself to be particularly successful when the photosensitizer is associated with nanoscaled carriers. This study aimed to design and develop a new formulation based on albumin nanospheres containing zinc-phthalocyanine tetrasulfonate (ZnPcS4-AN) for use in the PDT protocol and to investigate its antitumor activity in Swiss albino mice using the Ehrlich solid tumor as an experimental model for breast cancer.MethodsEhrlich tumor’s volume, histopathology and morphometry were used to assess the efficacy of intratumoral injection of ZnPcS4-AN in containing tumor aggressiveness and promoting its regression, while the toxicity of possible treatments was assessed by animal weight, morphological analysis of the liver and kidneys, hemogram, and serum levels of total bilirubin, direct bilirubin, indirect bilirubin, aspartate aminotransferase (AST), alanine aminotransferase (ALT), gamma glutamyl transferase (GGT), alkaline phosphatase, creatinine and urea. In order to evaluate the efficacy of PDT, groups of animals treated with intratumoral injection of doxorubicin (Dox) were also investigated.ResultsIntratumoral injection of ZnPcS4-AN was found to be efficient in mediating PDT to refrain tumor aggressiveness and to induce its regression. Although tumor volume reduction was not significant, PDT induced a remarkable increase in the necrosis area seen in the tumor’s central region, as in other experimental groups, including tumor and Dox treated groups, but also in the tumor’s peripheral region. Further, PDT showed minimal adverse effects. Indeed, the use of ZnPcS4-AN in mediating PDT revealed anti-neoplastic activity similar to that obtained while using intratumoral Dox therapy.ConclusionsPDT mediated by the new formulation ZnPcS4-AN enhanced the inhibition of tumor growth while producing practically no adverse effects and thus emerges as a very promising nanotechnology-based strategy for solid cancer treatment.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Antitumor activity of photodynamic therapy performed with nanospheres containing zinc-phthalocyanine

et al. 2013

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“…A new point of view offered the extraction mode of Lu et al [19] with a two-phase-extraction system to separate interfering lipophilic substances in organs, especially liver tissue. This method gave also the possibility to precipitate the protein fraction and save MTO from decomposition.…”

Section: Resultsmentioning

confidence: 99%

“…Moreover there are established protocols to determine MTO by HPLC measurements in context of drug delivery systems like liposome carriers [18] and nanosphere vehicles [19]. Protein precipitation with 0.5 M hydrochloric acid: acetonitrile (90 : 10, v/v), as described by Johnson et al [18], leads to a recovery of pure MTO in porcine muscle tissue of more than 85% (Table 1).…”

Section: Introductionmentioning

confidence: 99%

“…Lu et al developed a helpful method to determine MTO [19], which has been applied in the form of bovine serum albumin (BSA) nanospheres and revealed also promising results in our context. But in order to reliably capture even trace amounts of MTO, an optimization of the extraction mode has been necessary, as well as the development of a subsequent solid phase extraction (SPE) method.…”

Section: Introductionmentioning

confidence: 99%

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Mitoxantrone Loaded Superparamagnetic Nanoparticles for Drug Targeting: A Versatile and Sensitive Method for Quantification of Drug Enrichment in Rabbit Tissues Using HPLC-UV

Tietze

Schreiber

Lyer

2010

Journal of Biomedicine and Biotechnology

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In medicine, superparamagnetic nanoparticles bound to chemotherapeutics are currently investigated for their feasibility in local tumor therapy. After intraarterial application, these particles can be accumulated in the targeted area by an external magnetic field to increase the drug concentration in the region of interest (Magnetic-Drug-Targeting). We here present an analytical method (HPLC-UV), to detect pure or ferrofluid-bound mitoxantrone in a complex matrix even in trace amounts in order to perform biodistribution studies. Mitoxantrone could be extracted in high yields from different tissues. Recovery of mitoxantrone in liver tissue (5000 ng/g) was 76 ± 2%. The limit of quantification of mitoxantrone standard was 10 ng/mL ±12%. Validation criteria such as linearity, precision, and stability were evaluated in ranges achieving the FDA requirements. As shown for pilot samples, biodistribution studies can easily be performed after application of pure or ferrofluid-bound mitoxantrone.

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Heparin/chitosan nanoparticle carriers prepared by polyelectrolyte complexation

Liu

Jiao

Liu

et al. 2007

J Biomedical Materials Res

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In this study, novel nanoparticles were prepared by polyelectrolyte complexation between heparin and chitosan on simple and mild conditions. The size, polydispersity, zeta potential, and morphology of the nanoparticles were characterized. Entrapment studies of the nanoparticles were conducted using bovine serum albumin (BSA) as a model protein. Specifically, the effects of the pH value of chitosan solution, chitosan molecular weight (MW), chitosan concentration, heparin concentration, and BSA concentration on the nanoparticle size, the nanoparticle yield, and BSA entrapment were studied in detail. We found that, the size and the yield of the nanoparticles were affected by the above factors. The nanoparticle yield played a crucial role in BSA entrapment, namely, more nanoparticles could encapsulate more BSA. At length, suitably high pH value of chitosan solution, moderate chitosan MW, increasing both heparin concentration and chitosan concentration at an optimal concentration ratio favored more nanoparticles formed and consequently a higher BSA entrapment efficiency.

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Mitoxantrone-loaded BSA nanospheres and chitosan nanospheres for local injection against breast cancer and its lymph node metastases

Cited by 19 publications

References 7 publications

Antitumor activity of photodynamic therapy performed with nanospheres containing zinc-phthalocyanine

Antitumor activity of photodynamic therapy performed with nanospheres containing zinc-phthalocyanine

Mitoxantrone Loaded Superparamagnetic Nanoparticles for Drug Targeting: A Versatile and Sensitive Method for Quantification of Drug Enrichment in Rabbit Tissues Using HPLC-UV

Heparin/chitosan nanoparticle carriers prepared by polyelectrolyte complexation

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