MMP9 mRNA is a potential diagnostic and treatment monitoring marker for PTSD: Evidence from mice and humans

Chevalier, Céleste; Krampert, Luka; Schreckenbach, Monika; Schubert, Christine F.; Reich, Johanna; Novak, Božidar; Schmidt, Mathias; Rutten, Bart P. F.; Schmidt, Ulrike

doi:10.1016/j.euroneuro.2021.04.014

Cited by 7 publications

(6 citation statements)

References 45 publications

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“…So effective therapy for PTSD is still promoted. Biological markers have the potential to greatly simplify PTSD diagnosis, while also PTSD treatment response prediction and tracking [24]. In this study, we attempted to nd out biological markers and excavated possibly molecular mechanisms relevant to PTSD.…”

Section: Discussionmentioning

confidence: 99%

Identification of Critical Signature in Posttraumatic Stress Disorder Using Bioinformatics Analysis and Vitro Analyses

Liu,

et al. 2024

Preprint

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Background: Post-traumatic stress disorder (PTSD) is a complicated psychiatric disorder after experiencing trauma that affects people’s natural lives. As such, the objective of the study was mining related pathways and key genes of PTSD validated through vitro experiments. Method: Retrieving RNA sequencing (RNA-seq) datasets from the Gene Expression Omnibus (GEO) database. The differentially expressed genes (DEGs) were screened using GEO2R. KEGG pathway assessment was carried out for enrichment analysis. The plugin MCODE from Cytoscape software was utilized to analyze the protein-protein interaction (PPI) network. Assessment of anxiety and depression in a mice model of stress was carried out by the open-field test (OFT) and elevated plus maze test (EPMT). Subsequently, Real-Time quantitative PCR (qRT-PCR) was used to verify these key genes in stress-exposed models. Results: A total of 157 common upregulated DEGs and 53 common downregulated DEGs were identified between the amygdala (AMY) and the hippocampus (HIP). Notably enriched pathways (Neuroactive ligand-receptor interaction, mTOR signaling pathway, Nicotine addiction and dopaminergic synapse, etc.) were screened by Gene functional annotations and enrichment analysis. The PPI network identified the 4 hub genes , finding that the closely related pathways were Nicotine addiction and dopaminergic synapse. qRT-PCR was used to confirm these four genes and the expressing trends were consistent with the microarray analysis. The results of the OFT and the EMPT were remarkable changes. Conclusion: Using bioinformatic analysis and in vitro experiments, we explored a variety of genes and pathways that may represent key mechanisms in the development of PTSD.

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Section: Discussionmentioning

confidence: 99%

Identification of Critical Signature in Posttraumatic Stress Disorder Using Bioinformatics Analysis and Vitro Analyses

Liu,

et al. 2024

Preprint

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“…In studies of ICH and ischemic stroke (IS), Il1r2 has been implicated as an early response gene [ 5 , 33 ]. Furthermore, IL1R2 is a diagnostic marker for acute myocardial infarction (AMI) and post-traumatic stress disorder (PTSD) [ 34 , 35 ]. Moreover, TLR family proteins can also trigger inflammation.…”

Section: Discussionmentioning

confidence: 99%

Consistency of mouse models with human intracerebral hemorrhage: core targets and non-coding RNA regulatory axis

Jin,

Meng,

Zhang

et al. 2024

Aging

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Intracerebral hemorrhage (ICH) has a high mortality and disability rate. Numerous basic studies on pathogenesis and therapeutics have been performed in mice. However, the consistency of the experimental mouse model and the human ICH patient remains unclear. This has slowed progress in translational medicine. Furthermore, effective therapeutic targets and reliable regulatory networks for ICH are needed. Therefore, we determined the differentially expressed (DE) messenger RNAs (mRNAs), microRNAs (miRNAs) and circular RNAs (circRNAs) before and after murine ICH and analyzed their regulatory relationships. Subsequently, data on mRNAs from human peripheral blood after ICH were obtained from the Gene Expression Omnibus database. The DE mRNAs after human ICH were compared with those of the mouse. Finally, we obtained seven genes with translational medicine research value and verified them in mice. Then the regulatory network of these genes was analyzed in humans. Similarly, species homologies of these regulatory pathways were identified. In conclusion, we found that the mouse ICH model mimics the human disease mainly in terms of chemokines and inflammatory factors. This has important implications for future research into the mechanisms of ICH injury and repair.

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“…In the study of ICH and CI, It is considered to be an early response gene 28,29 . IL1R2 has been identi ed as a diagnostic marker of acute myocardial infarction (AMI) and post-traumatic stress disorder (PTSD) 30,31 . Numerous studies have been carried out on Toll like receptor 4 (TLR4) in ICH.…”

Section: Discussionmentioning

confidence: 99%

Regulatory Network after Intracerebral Hemorrhage

Jin

Meng

Zhang

et al. 2022

Preprint

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Intracerebral hemorrhage (ICH) has a high mortality and disability rate. As it is difficult to obtain brain tissue of patients occurred ICH, the mechanism research carried out primarily through animal experiments, and the translational medicine progresses slowly. More effective therapeutic targets and reliable regulatory networks for ICH are needed. We detected the expression levels of mRNAs, miRNAs and circRNAs before and after ICH, then added lncRNAs to obtain a more complete regulatory network. Subsequently, an original mRNA data in human peripheral blood after ICH from the GEO database was obtained. After analyzing the differential expressed mRNAs of the data, it was intersected with the mouse data. Then seven genes have been found to have made significant changes in both humans and mice after ICH. Finally, these gene regulatory networks in humans were analyzed. By comparing species, we provided signaling pathways for the human and mouse homologous in these regulatory networks. Hoped that some of them might become early warning signals of the ICH. Finally, the tendency of change in RNA levels of the above seven genes was checked after ICH in the mouse brain, in order to provide more valuable data.

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MMP9 mRNA is a potential diagnostic and treatment monitoring marker for PTSD: Evidence from mice and humans

Cited by 7 publications

References 45 publications

Identification of Critical Signature in Posttraumatic Stress Disorder Using Bioinformatics Analysis and Vitro Analyses

Identification of Critical Signature in Posttraumatic Stress Disorder Using Bioinformatics Analysis and Vitro Analyses

Consistency of mouse models with human intracerebral hemorrhage: core targets and non-coding RNA regulatory axis

Regulatory Network after Intracerebral Hemorrhage

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