MnFe₂O₄ nanoclusters as labels for the quantitative detection of D‐dimer in a lateral‐flow immunochromatographic assay

Abstract

In this study, MnFe 2 O 4 nanoclusters were prepared as bioprobes to establish a lateral-flow immunochromatographic assay (LFIA) for the rapid and quantitative detection of D-dimer for the first time. The magnetic properties of the magnetic labels play a key role in the quantitative detection of biomolecules. The 47.3-nm MnFe 2 O 4 magnetic nanoclusters (MNCs) with good dispersion and high saturation magnetization (76 emu/g) were fabricated via thermal decomposition of Fe(acac) 3 with Mn(acac) 2 . The prepared MnFe 2 O 4 MNCs were well dispersed in water because the surfaces were fully covered with 3,4-dihydroxyhydrocinnamic acid (DHCA) molecules by ligand exchange. Anti-D-dimer antibodies were coupled on the surface of MnFe 2 O 4 MNCs, and the target protein, D-dimer, was detected, in the range 0.05-6 μg/mL. This assay provides a promising platform for D-dimer detection for point-of-care diagnosis.

Magnetic Lateral Flow Immunoassays

“…Regarding the crystal structure of magnetic nanoparticles, magnetite (Fe 3 O 4 ) has been the preferred option in order to develop LFIA. Li et al [83] have used MnFe 2 O 4 instead of magnetite in order to achieve stronger magnetization. They hypothesized that the hybridization of iron and manganese atoms increased the spin magnetic moment between them, resulting in a higher total magnetic moment for the nanoparticle.…”