Biology learning on material that contains abstract concepts with high competency demands that require a scientific process / hands-on experience such as practicum to concretize and fulfill it is not optimally fulfilled due to limitations of space, time, activity, sensory power, and facilities, especially currently there is an adjustment in learning due to The Covid 19 pandemic. This study aims to develop an Interactive Learning Media, namely a Virtual Laboratory in .Apk format that can run on Android Smartphones that are valid, practical, and effective in supporting the learning of Biology material on the Food Digestive System class XI MIPA at SMAN 1 Abung Semuli, North Lampung. This study uses the 4D Thiagarajan Research and Development (R&D) method combined with the Luther-Sutopo Multimedia Development Life Cycle (MDLC) Development Model. Through the research stages, a 13 Mb .apk was generated containing the Intro, Home, Instructions, Developer, KD/Objectives, Materials, Practicum, and Exercise pages. Based on the analysis of the Test Result Data to Experts and Users, the Application is declared Valid, Practical, and Effectively used by Biology Teachers and Students of class XI MIPA in supporting Biology learning materials for the Food Digestive System. With a validity value of 0.85 in the valid criteria, practicality 0.94 in the very practical criteria, the effectiveness value is 94.46 in the very effective criteria.