Mobilization of early hematopoietic progenitor cells with BB-10010: a genetically engineered variant of human macrophage inflammatory protein- 1 alpha

Lord, Brian I; Woolford, LB; Wood, LM; Czaplewski, L. G.; McCourt, Matthew; Hunter, M. G.; Edwards, RM

doi:10.1182/blood.v85.12.3412.bloodjournal85123412

Cited by 111 publications

(44 citation statements)

References 5 publications

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“…In the mouse system it is clear that the spleen has an important role in the control of the peripheral blood cell population after denervation, as has been seen with cytokine-stimulated progenitor cell mobilization and leucocyte numbers (Molineux et al, 1990a, b;Lord et al, 1995) and as has been reported in humans after splenectomy (McBride et al, 1968). In our experiments, as in those reported by others, splenectomy apparently unmasks the full extent of peripheral blood mobilization of progenitor cells.…”

Section: Discussionsupporting

confidence: 86%

Bone marrow innervation regulates cellular retention in the murine haemopoietic system

Afan¹,

Broome²,

Nicholls

et al. 1997

Br J Haematol

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Summary. An anatomical analysis of the innervation of murine femora revealed intimate association of haemopoietic and stromal cells with nerve fibres. The mechanical denervation of these femora resulted in significant mobilization of cells into the peripheral blood within 24 h. There was a decrease in femoral cellularity and analysis of the type of cells mobilized also revealed that there was an increase in progenitor cells in the peripheral blood. In non-splenectomized mice these progenitor cells were quickly cleared from the circulation. Chemical sympathectomy of mice with 6-hydroxydopamine resulted in decreased bone marrow cellularity without a change in bone marrow or peripheral blood progenitor cell numbers, nor the sustained rise in peripheral leucocytes observed with whole nerve denervation. These observations argue for selective control of mobilization by the nervous system and also indicate possible control of proliferation within the bone marrow. We conclude that the innervation has an important role in the maintenance of the blood-marrow interface, control of peripheral blood cell numbers, and mobilization of colony forming cells into the periphery.

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Section: Discussionsupporting

confidence: 86%

Bone marrow innervation regulates cellular retention in the murine haemopoietic system

Afan¹,

Broome²,

Nicholls

et al. 1997

Br J Haematol

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“…MIP-1␣/CCL3 was one of the first members of the chemokine family to be used to mobilize HPCs and HSCs to the blood of mice. 30 This mobilization is mediated through chemokine receptor CCR1, 32 which is one of the receptors that bind MIP-1␣. Mobilization of HPCs in mice with MIP-1␣/CCL3 is potent and rapid, with maximal effects occurring within 1 h. We reasoned that mobilization with MIP-1␣/CCL3 might be different in mechanism than that of AMD3100, which works through antagonizing the binding of SDF-1/CXCL12 to its receptor, CXCR4, 14 and also that of G-CSF, which occurs as a much more prolonged response after days of multiple administrations of G-CSF.…”

Section: Effect Of Mip-1␣/ccl3 On the Mobilization Of Hpcs Alone Andmentioning

confidence: 99%

AMD3100 and CD26 Modulate Mobilization, Engraftment, and Survival of Hematopoietic Stem and Progenitor Cells Mediated by the SDF‐1/CXCL12‐CXCR4 Axis

Broxmeyer

Hangoc

Cooper

et al. 2007

Annals of the New York Academy of Sciences

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The chemokine stromal cell-derived factor-1 (SDF-1/CXCL12) and its receptor, CXCR4, are involved in a number of facets of the regulation of hematopoiesis at the level of hematopoietic stem (HSCs) and progenitor (HPCs) cells. Modulation of this ligand-receptor interaction may be of clinical utility. We now report that: (1) the CC chemokine, macrophage inflammatory protein-1alpha (MIP-1alpha/CCL3) synergizes with AMD3100 (an antagonist of the binding of SDF-1/CXCL12 to CXCR4) to rapidly mobilize HPCs to the blood of mice; moreover, the combination of granulocyte colony-stimulating factor (G-CSF) with AMD3100 and MIP-1alpha/CCL3, given in a specific sequence, mobilizes the greatest number of HPCs compared to any combination of two of these mobilizing agents; (2) pretreatment of recipient mice with Diprotin A, an inhibitor of CD26/Dipeptidylpeptidase IV (DPPIV), enhances the competitive HSCs repopulating capacity of untreated donor cells; (3) the survival-enhancing effects of SDF-1/CXCL12 on HPCs subjected in vitro to delayed addition of growth factors (GFs) are mediated in part through the cell cycle-related proteins p21(cip1/waf1) (as assessed using p21(cip1/waf1) -/- and +/+ mice) and Mad2 (using Mad2 +/- and +/+ mice); and (4) deletion of CD26/DPPIV on mouse bone marrow cells increases the survival-enhancing effects of SDF-1/CXCL12 on HPCs. These results demonstrate the means to increase the mobilization of HPCs, the engrafting capability of HSCs, and responsiveness of HPCs to the survival-enhancing activity of SDF-1/CXCL12, effects that may be of practical value.

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“…Murine HSC capable of reconstituting a myeloablated animal can be harvested from cytokine mobilized peripheral blood in much the same way as in humans (Molineux et al, 1990). Briddell et al (1993) Georges et al, 1998) Mobilizing regimen Reference G-CSF Briddell et al (1993) Cyclophosphamide Neben et al (1993) Stem cell factor (SCG) Mauch et al (1995) Stem cell factor + G-CSF Briddell et al (1993) IL-1β Fibbe et al (1992) IL-6 Suzuki et al (1995) IL-6 + G-CSF Suzuki et al (1995) IL-7 Grzegorzewski et al (1995) IL-8 Laterveer et al (1995) IL-11 Mauch et al (1995) IL-11 + SCF Mauch et al (1995) FLT-3 + G-CSF Sudo et al (1997) MIP-1α Lord et al (1995) MIP-1α + G-CSF Lord et al (1995) mice transplanted with 500,000 donor G-CSFmobilized peripheral-blood low-density cells. Donor mice received seven daily injections of human G-CSF at a dose of 200 µg/kg.…”

Section: Mobilized Peripheral Bloodmentioning

confidence: 99%

Sources of Human and Murine Hematopoietic Stem Cells

Horwitz

2007

CP in Immunology

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This unit describes various sites from which human and murine hematopoietic stem cells (HSC) can be collected, including bone marrow, peripheral blood, and, in the case of human HSC, umbilical cord blood. Guidelines related to each of the cell harvesting procedures are described, and a discussion of the expected populations of cells harvested from these various sites is included. Cell products can be composed of a combination of true HSC, lineage-committed hematopoietic progenitor cells, and terminally differentiated fully mature cells. While use of steady-state peripheral blood as a source of HSC is the most convenient approach, yields of these cells are very low. However, mobilization methods that stimulate movement of stem cells out of the bone marrow into peripheral blood significantly increase HSC yields. Use of G-CSF has been accepted as the standard method for stem cell mobilization. The unit describes a variety of investigational cytokines and chemokines that have been shown to mobilize human stem including interleukin 3, 11, and 8, flt-3 ligand, and stem cell factor.

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Mobilization of early hematopoietic progenitor cells with BB-10010: a genetically engineered variant of human macrophage inflammatory protein- 1 alpha

Cited by 111 publications

References 5 publications

Bone marrow innervation regulates cellular retention in the murine haemopoietic system

Bone marrow innervation regulates cellular retention in the murine haemopoietic system

AMD3100 and CD26 Modulate Mobilization, Engraftment, and Survival of Hematopoietic Stem and Progenitor Cells Mediated by the SDF‐1/CXCL12‐CXCR4 Axis

Sources of Human and Murine Hematopoietic Stem Cells

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