Mobilization of Mesenchymal Stem Cells by Granulocyte Colony-stimulating Factor in Rats with Acute Myocardial Infarction

Cheng, Zhaokang; Ou, Lailiang; Zhou, Xin; Liu, Yi; Jia, Xiaohua; Zhang, Jin; Li, Yuming

doi:10.1007/s10557-008-6110-2

Cited by 34 publications

(24 citation statements)

References 39 publications

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“…Mesenchymal stem cells are undifferentiated pluripotential cells present in bone marrow, capable of differentiating into many cell types (chondrogenic, adipogenic, or osteogenic lineages) and may be a suitable autogenous cell source for tissue regeneration. Together with cells coming from the blood, the MSCs are the cells responsible for the first contact with the surface of titanium implants [39][40][41] . The adhesion of these cells to a biomaterial surface can be a major factor mediating its biocompatibility.…”

Section: Discussionmentioning

confidence: 99%

In vitro osteoinduction of human mesenchymal stem cells in biomimetic surface modified titanium alloy implants

Santander¹,

Alcaine²,

Lyahyai³

et al. 2012

Dent. Mater. J.

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Section: Discussionmentioning

confidence: 99%

In vitro osteoinduction of human mesenchymal stem cells in biomimetic surface modified titanium alloy implants

Santander¹,

Alcaine²,

Lyahyai³

et al. 2012

Dent. Mater. J.

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“…The heart weight in CHF rats increased, but MSCs did not affect this outcome. Nevertheless, despite their effect on morphological structure of the heart, MSCs did not improve the cardiac function, which still remained depressed in CHF rats treated with MSCs; this finding is consistent with other studies [28][29][30].…”

Section: Discussionsupporting

confidence: 92%

Mesenchymal Stem Cells Improve Heart Rate Variability and Baroreflex Sensitivity in Rats with Chronic Heart Failure

Morais

Silva

Lataro

et al. 2015

Stem Cells and Development

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Heart failure induced by myocardial infarct (MI) attenuates the heart rate variability (HRV) and baroreflex sensitivity, which are important risk factors for life-threatening cardiovascular events. Therapies with mesenchymal stem cells (MSCs) have shown promising results after MI. However, the effects of MSCs on hemodynamic (heart rate and arterial pressure) variability and baroreflex sensitivity in chronic heart failure (CHF) following MI have not been evaluated thus far. Male Wistar rats received MSCs or saline solution intravenously 1 week after ligation of the left coronary artery. Control (noninfarcted) rats were also evaluated. MI size was assessed using single-photon emission computed tomography (SPECT). The left ventricular ejection fraction (LVEF) was evaluated using radionuclide ventriculography. Four weeks after MSC injection, the animals were anesthetized and instrumented for chronic ECG recording and catheters were implanted in the femoral artery to record arterial pressure. Arterial pressure and HRVs were determined in time and frequency domain (spectral analysis) while HRV was also examined using nonlinear methods: DFA (detrended fluctuation analysis) and sample entropy. The initial MI size was the same among all infarcted rats but was reduced by MSCs. CHF rats exhibited increased myocardial interstitial collagen and sample entropy combined with the attenuation of the following cardiocirculatory parameters: DFA indices, LVEF, baroreflex sensitivity, and HRV. Nevertheless, MSCs hampered all these alterations, except the LVEF reduction. Therefore, 4 weeks after MSC therapy was applied to CHF rats, MI size and myocardial interstitial fibrosis decreased, while baroreflex sensitivity and HRV improved.

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“…Our previous results showed that increased surface CXCR4 expression was associated with enhanced MSC migration (Cheng et al, 2008a(Cheng et al, , 2008c. To determine whether SDF-1 enhanced MSC migration through modulation of cell surface CXCR4 expression, MSCs were incubated with SDF-1 before hypoxia/re-oxygenation for 16 h. Flow cytometry analysis showed that SDF-1 tended to increase surface CXCR4 expression compared to non-treated cells, but this did not reach statistical significance ( SDF-1 promotes the secretion of bFGF and VEGF through the SDF-1/CXCR4 axis Paracrine secretion of basic fibroblast growth factors (bFGF) and vascular endothelial growth factor (VEGF) is crucial for protecting MSCs in ischemic tissues (Kinnaird et al, 2004a).…”

Section: Effects Of Sdf-1 Pretreatment On Msc Surface Cxcr4 Expressionmentioning

confidence: 95%

“…Surface CXCR4 expression was analyzed as previously described (Cheng et al, 2008a). Briefly, cells were stained with rabbit anti-CXCR4 antibody (Thermo Fisher Scientific, Fremont, CA, USA) followed by Alexa Fluor 594 goat anti-rabbit IgG (Molecular Probes, Eugene, OR, USA).…”

Section: Flow Cytometry Analysis Of Surface Cxcr4 Expressionmentioning

confidence: 99%

“…Our previous study showed that overexpression of CXCR4 increased engraftment of MSCs in the infarcted myocardium and improved cardiac performance (Cheng et al, 2008c). We also demonstrated that granulocyte colony-stimulating factor (G-CSF) mobilized MSCs to the infarcted heart through the upregulation of CXCR4 (Cheng et al, 2008a). Beside the role in migration, the SDF-1/CXCR4 axis is also important for cell survival.…”

Section: Introductionmentioning

confidence: 99%

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SDF-1/CXCR4 axis modulates bone marrow mesenchymal stem cell apoptosis, migration and cytokine secretion

et al. 2011

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Bone marrow mesenchymal stem cells (MSCs) are considered as a promising cell source to treat the acute myocardial infarction. However, over 90% of the stem cells usually die in the first three days of transplantation. Survival potential, migration ability and paracrine capacity have been considered as the most important three factors for cell transplantation in the ischemic cardiac treatment. We hypothesized that stromal-derived factor-1 (SDF-1)/CXCR4 axis plays a critical role in the regulation of these processes. In this study, apoptosis was induced by exposure of MSCs to H 2 O 2 for 2 h. After re-oxygenation, the SDF-1 pretreated MSCs demonstrated a significant increase in survival and proliferation. SDF-1 pretreatment also enhanced the migration and increased the secretion of pro-survival and angiogenic cytokines including basic fibroblast growth factor and vascular endothelial growth factor. Western blot and RT-PCR demonstrated that SDF-1 pretreatment significantly activated the pro-survival Akt and Erk signaling pathways and up-regulated Bcl-2/Bax ratio. These protective effects were partially inhibited by AMD3100, an antagonist of CXCR4. We conclude that the SDF-1/CXCR4 axis is critical for MSC survival, migration and cytokine secretion.

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Mobilization of Mesenchymal Stem Cells by Granulocyte Colony-stimulating Factor in Rats with Acute Myocardial Infarction

Abstract: G-CSF enhanced the migration of systemically delivered MSCs from bone marrow to infarcted heart. However, the beneficial effect of this kind of migration is limited, as cardiac function did not improve.

Cited by 34 publications

References 39 publications

In vitro osteoinduction of human mesenchymal stem cells in biomimetic surface modified titanium alloy implants

In vitro osteoinduction of human mesenchymal stem cells in biomimetic surface modified titanium alloy implants

Mesenchymal Stem Cells Improve Heart Rate Variability and Baroreflex Sensitivity in Rats with Chronic Heart Failure

SDF-1/CXCR4 axis modulates bone marrow mesenchymal stem cell apoptosis, migration and cytokine secretion

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