This study aims to assess the relationship between the surface roughness of cotton plain-woven fabric before and after each pretreatment processing stage. According to the findings of this study, the surface roughness values of desized fabric increased in the warp and weft directions. On the other hand, the roughness of plain-woven fabric reduced in both the warp and weft directions during the scouring, bleaching, and mercerization processes. The weft roughness values of greige, scoured, bleached, and mercerized fabrics are higher than the warp roughness values. Mercerized fabrics provide the smoothest fabric surface compared to other treatment stages. The pretreatment stages have statistically significant impacts on the surface roughness in both the warp and weft directions at a 95% confidence interval. The pairwise analysis reveals that pretreatment of the fabrics in the warp and weft directions is statistically significant for grey with desizing, grey with scouring, grey with bleaching, grey with mercerizing, desizing with scouring, desizing with bleaching, desizing with mercerization, scouring with mercerization, and bleaching with mercerization, but not for scouring with bleaching. Mercerized fabric is highly recommended for producing textile products with unique requirements in terms of tactilely sensitive surface properties.