Modelling the effect of chlorination/chloramination on induction of viable but non-culturable (VBNC) <i>Escherichia coli</i>

Chen, Sheng; Zeng, Jie; Wang, Yahong; Ye, Chengsong; Zhu, Shuai; Liu, Feng; Zhang, Shenghua; Yu, Xin

doi:10.1080/09593330.2019.1611939

Cited by 15 publications

(9 citation statements)

References 41 publications

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“…However, it is not always possible to separately quantify the culturable and VBNC bacterial fraction in each sample. To address this problem, several studies have evaluated and modeled the induction of VBNC state in culturable bacteria under disinfectants like chlorine, chloramine (S. Chen et al, 2019;Oliver, Dagher, & Linden, 2005) and UV (Shenghua Zhang, Ye, Lin, Lv, & Yu, 2015). As the majority of environmental bacteria belong to VBNC, these models combined with the data on culturable ARB quantities may be usable in approximating the VBNC fraction of ARBs in a sample and in turn in evaluating the health risks.…”

Section: What Is the Effect Of Vbnc Status On Antibiotic Resistance?mentioning

confidence: 99%

Understanding human health risks caused by antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARG) in water environments: Current knowledge and questions to be answered

Amarasiri

Sano

Suzuki

2019

Critical Reviews in Environmental Science and Technology

428

166

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Section: What Is the Effect Of Vbnc Status On Antibiotic Resistance?mentioning

confidence: 99%

Understanding human health risks caused by antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARG) in water environments: Current knowledge and questions to be answered

Amarasiri

Sano

Suzuki

2019

Critical Reviews in Environmental Science and Technology

428

166

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“…HOCl and NaOCl also induce the viable but nonculturable (VBNC) state in bacterial cells [ 176 , 179 , 180 ]. This condition is a bacterial response to hostile environments in which they enter in a dormant state where the cells remain viable but do not grow under standard culture conditions [ 181 ].…”

Section: Adaptive Response Of Gram-negative Cells To Hoclmentioning

confidence: 99%

Surviving Reactive Chlorine Stress: Responses of Gram-Negative Bacteria to Hypochlorous Acid

2020

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Sodium hypochlorite (NaOCl) and its active ingredient, hypochlorous acid (HOCl), are the most commonly used chlorine-based disinfectants. HOCl is a fast-acting and potent antimicrobial agent that interacts with several biomolecules, such as sulfur-containing amino acids, lipids, nucleic acids, and membrane components, causing severe cellular damage. It is also produced by the immune system as a first-line of defense against invading pathogens. In this review, we summarize the adaptive responses of Gram-negative bacteria to HOCl-induced stress and highlight the role of chaperone holdases (Hsp33, RidA, Cnox, and polyP) as an immediate response to HOCl stress. We also describe the three identified transcriptional regulators (HypT, RclR, and NemR) that specifically respond to HOCl. Besides the activation of chaperones and transcriptional regulators, the formation of biofilms has been described as an important adaptive response to several stressors, including HOCl. Although the knowledge on the molecular mechanisms involved in HOCl biofilm stimulation is limited, studies have shown that HOCl induces the formation of biofilms by causing conformational changes in membrane properties, overproducing the extracellular polymeric substance (EPS) matrix, and increasing the intracellular concentration of cyclic-di-GMP. In addition, acquisition and expression of antibiotic resistance genes, secretion of virulence factors and induction of the viable but nonculturable (VBNC) state has also been described as an adaptive response to HOCl. In general, the knowledge of how bacteria respond to HOCl stress has increased over time; however, the molecular mechanisms involved in this stress response is still in its infancy. A better understanding of these mechanisms could help understand host-pathogen interactions and target specific genes and molecules to control bacterial spread and colonization.

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“…The main advantages of using flow cytometry for community composition monitoring lies in the speed (i.e., minutes) and the high potential for automation ( 43 , 44 ), which enables monitoring with high temporal resolution. Additionally, the independence of cultivation is a great advantage for monitoring managed ecosystems, since human-induced stressors, such as disinfection, are known to induce viable but nonculturable (VBNC) states ( 45 ). Practical applications of the pipeline can include monitoring the efficacy of management strategies, follow-up outbreaks of unwanted bacterial taxa, monitoring the presence of probiotic strains, etc.…”

Section: Discussionmentioning

confidence: 99%

Predicting the Presence and Abundance of Bacterial Taxa in Environmental Communities through Flow Cytometric Fingerprinting

et al. 2021

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Modelling the effect of chlorination/chloramination on induction of viable but non-culturable (VBNC) Escherichia coli

Cited by 15 publications

References 41 publications

Understanding human health risks caused by antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARG) in water environments: Current knowledge and questions to be answered

Understanding human health risks caused by antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARG) in water environments: Current knowledge and questions to be answered

Surviving Reactive Chlorine Stress: Responses of Gram-Negative Bacteria to Hypochlorous Acid

Predicting the Presence and Abundance of Bacterial Taxa in Environmental Communities through Flow Cytometric Fingerprinting

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