Modification of methods for detection of Escherichia coli O157:H7 on produce

Kim, Se-Ri; Yoon, Yohan; Seo, Min-Kyoung; Kim, Won-Il; Shim, Won‐Bo; Chung, Donghun; Yun, Jong Chul; Ryu, Ki‐Hyun; Kim, Il‐Kyu

doi:10.1007/s10068-014-0185-2

Cited by 2 publications

(2 citation statements)

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“…In fact, the current intensity in the presence of E. coli was almost 100-fold higher than that in the presence of other bacteria. The present technique provided better selectivity compared to other E. coli O157:H7 sensing assays [9]. Such a high selectivity of the microelectrode immunosensor can contribute to the specific immunoreaction.…”

Section: Performance Of the E Coli O157:h7 Detection Systemmentioning

confidence: 73%

“…Towards this goal, many approaches have been developed for E. coli O157:H7 detection, such as culturebased method, polymerase chain reaction (PCR)-based technique, gold nanoparticle-labelled lateral flow immunoassay and matrix-assisted laser desorption ionization mass spectrometry [6][7][8][9][10][11][12]. For instance, MacConkey medium containing sorbitol, not lactose, is widely used to score E. coli O157:H7 counts [9], followed by CONTACT Le Li 32667661@qq.com; leliscu@outlook.com, Jinfeng Zhao 447620788g@qq.com; zhaojinfeng@hotmail.com serological verification with O157-and H7-specific antisera, which is one of the traditional laboratory methods. However, for some strains, failure to produce b-glucuronidase would cause false negatives.…”

Section: Introductionmentioning

confidence: 99%

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Highly selective detection of Escherichia coli O157:H7 based on micro-gapped interdigitated electrode arrays

Chen

Wang

et al. 2017

Biotechnology & Biotechnological Equipment

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Development of effective pathogen detection systems is critical for human health. In this study, a micro-gapped interdigitated electrode array-based immunosensor chip is constructed for the detection of a typical pathogen, Escherichia coli (E. coli) O157:H7. The immunosesnor chip was prepared via photolithography and characterized by a digital camera, optical microscope and scanning electron microscope. The E. coli O157:H7 detection mechanism is based on an enzymelinked, sandwiched immunoassay with enzymatic silver deposition. Our results revealed that the proposed immunosensor chip showed selective electrical response towards E. coli O157:H7 over other bacteria including Hemolytic streptococcus, Staphylococcus aureus, Salmonella, Pseudomonas aeruginosa, Chronobacter sakazakii and Bacillus cereus. Our assay exhibited potential practical applications for E. coli O157:H7 determination in water and our methodology might be applied to detect different pathogens by simply selecting specific antibodies. KEYWORDSEscherichia coli O157:H7; micro-gapped interdigitated electrode array; immunoassay; electrochemical sensing Abbreviations AAP ascorbic acid phosphate enzyme ALP alkaline phosphatase APTES 3-aminopropyl triethoxysilane BSA bovine serum albumin EDS energy-dispersive spectroscopy ELISA enzyme-linked immunosorbent assay LSV linear sweep voltammetry PBS phosphate-buffered saline SEM scanning electron microscope TB Tris-HCl buffer PBST phosphate-buffered saline tween

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Section: Performance Of the E Coli O157:h7 Detection Systemmentioning

confidence: 73%