Modification of polysulfone with phospholipid polymer for improvement of the blood compatibility. Part 2. Protein adsorption and platelet adhesion

On the Use of the Platelet Activity State Assay for the In Vitro Quantification of Platelet Activation in Blood Recirculating Devices for Extracorporeal Circulation

“…In fact, in the relatively complex blood flow path within a hollow fiber OXY device, flowing platelets are exposed to: (i) secondary flows and recirculation/ stagnation areas, such as those due to the gradual section reduction/enlargement of the inlet divergent and outlet convergent conduits, respectively; (ii) sudden changes of the velocity vectors (e.g., within the upstream and downstream manifolds of the inlet and outlet zones of the Kids D100) where the streamflow is driven circumferentially toward the hollow fiber bundle of the OXY module, and the outlet section, respectively [23]; (iii) anisotropic flow distribution across the array of the fiber bundle, where nonnegligible shear stress occurs due to the friction with the fiber walls, as reported in Pelosi et al (32). In addition, there is supporting evidence in literature that phosphorylcholine surface coating is effective in protecting platelets from elevated activation during ECC (3,(33)(34)(35). Thus, our experimental layout (comparison of PC vs. NC devices) allowed us to test the sensitivity of the assay to detect GFP-surface contact interactions under flow, in a convenient clinically pertinent in vitro setting.…”

Layer-by-Layer Building up of Redox Phospholipid Polymer Hydrogel Electrode for Biosensor

“…It has been reported that 2-methacryloyloxyethyl phosphorylcholine (MPC) polymers containing polar phospholipid groups in the side chain have excellent performance in inhibiting the non-specific adsorption of serum proteins, agglomeration of platelets or attachment of cells. [11][12][13][14] Also, the MPC polymer hydrogel membranes have good permeability to gas and solute. 15,16) Previous to this study, a hydrogel system formed by a MPC polymer and poly(vinyl alcohol) (PVA) had been prepared for cell culture 17) and controlled release of bioactive agents.…”

The effects of urease immobilization on the transport characteristics and protein adsorption capacity of cellulose acetate based hemodialysis membranes

“…To increase hydrophilic nature of the blood contacting membranes, numerous surface modification methods have been suggested which can be summarized as follows: 1) blending hydrophilic polymers such as 2-methacryloyloxyethyl phosphorylcholine (MPC) [2][3][4][5][6][7][8], polyvinylpyrrolidone (PVP) [9,10], or polyethylene oxide (PEO) [11,12] into the membrane forming solution, 2) grafting hydrophilic groups such as polyethylene glycol by UV-irradiation [13][14][15] or low temperature plasma technique [16][17][18][19], 3) graft copolymerization of monomers [20][21][22][23], 4) coating with hydrophilic polymers or copolymers [24][25][26][27][28][29]. Hemodialysis membranes were also immobilized with low molecular weight anticoagulant heparin to decrease protein adsorption capacities, hence, to improve hemocompatibilities [30][31][32][33][34][35][36][37].…”

“…Lin et al have found that adsorption of human serum albumin and human plasma fibrinogen on polyacrylonitrile membrane decreased when chitosan/heparin polyelectrolyte complex was immobilized on the surface [37]. This was attributed to electrostatic repulsion between SO 3 -and COO -groups on heparin and negatively charged proteins in normal blood circumstance (pH 7.4). An alternative approach to membrane surface modification is protein immobilization on the hemodialysis membranes [38,39].…”