Modification of stress tolerance by dehydration pretreatment in winter rape hypocotyls

Shcherbakova, A M; Kacperska‐Palacz, Alina

doi:10.1111/j.1399-3054.1980.tb03304.x

Cited by 18 publications

(8 citation statements)

References 10 publications

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“…Evidence for a hardening of rape shoots as a consequence of a desiccation experience was reported by Shcherbakova and Kacperska-Palacz (17). Our evidence of a reduced leakiness in cowpea leaf discs after being held in a partially desiccated state is consistent with the same interpretation, i.e.…”

Section: Methodssupporting

confidence: 82%

Solute Leakage Resulting from Leaf Desiccation

Leopold¹,

Musgrave²,

Williams³

1981

Plant Physiol.

139

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The leakage of solutes from foliar tissue is utilized as a dynamic measure of apparent changes in membrane integrity in response to desiccation. It is found that rehydrating leaf discs of cowpea (Vigna sixensis IL.l Endi.) show increasing leakiness in proportion to the extent of prior desiccation, whereas SelgineUa kpidophyla Spring., a resurrection plant, does not. RESULTS Using the A at 280 nm as a measure of the leakage of solutes from the leaf tissues immersed in water, one can compare the apparent relative leakiness from tissues that have been desiccated to various levels. Such an experiment for leaf discs of cowpea (a plant which is relatively susceptible to desiccation) and for frond pieces ofSelaginella (a plant with notable resistance to desiccation) is shown in Figure 1. When cowpea leaf discs had been dried to various degrees (90, 76, or 52% of original fresh weight), the subsequent leakage characteristics obtained from the discs in water show a period of relatively rapid loss of solutes lasting for about 4 or 5 min, and after that a relatively steady rate of leakage (Fig. IA). Both the slope of that leakage curve and the total amounts of solutes leaked out over a 20-min period indicate that, after greater amounts of desiccation, there is an increased amount of leakage. On the other hand, Selaginella was found to suffer no perceptable increase in leakage even when desiccation had advanced to 40o of initial fresh weight (Fig. IB).The relation of the extent of leakage and the extent of desiccation damage is further illustrated in Figure 2. When the total amount of leakage after 20 min is plotted against the extent of prior desiccation, it is evident that the cowpea leaf discs lose increasing amounts of solutes with greater extents of desiccation, whereas the Selaginella tissue does not.In the course of these experiments, it was found that differences in leakage rates were obtained as leaves of differing ages were used. A comparison of leaf discs desiccated to 55% of fresh weight and undesiccated discs of three different leaf ages are shown in Figure 3. In this case, the leakage is expressed as a percentage of total content of A28o-absorbing materials, since the total A2ws content increases with the age of the leaf. It can be seen that the extent of leakage increases with leaf age, and that the desiccation experience results in substantially greater solute leakage from

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Section: Methodssupporting

confidence: 82%

Solute Leakage Resulting from Leaf Desiccation

Leopold¹,

Musgrave²,

Williams³

1981

Plant Physiol.

139

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“…Electrolyte leakage, as determined in this study, has been considered as an indicator of plant dehydration tolerance [13,25]. Water stress conditioning did not induce significant differences in twig EL measured at a 100 % WC among conditioning treatments, which indicates that water stress does not enhance dehydration tolerance of Aleppo pine seedlings.…”

Section: Resultsmentioning

confidence: 79%

“…Among these, application of restricted watering has been proved to promote osmotic adjustments and changes in cell wall elasticity [9,14] and to increase root growth capacity [2,22]. It can also induce a reduction of the transpiration rate after drought recovery [7,30,37] and improve dehydration tolerance [25]. All these responses have been considered as mechanisms that may improve resistance of plants to water stress.…”

Section: Introductionmentioning

confidence: 99%

Effect of water stress conditioning on the water relations, root growth capacity, and the nitrogen and non-structural carbohydrate concentration of Pinus halepensis Mill. (Aleppo pine) seedlings

et al. 1999

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“…The dises were subscquently hydrated by floating on distilled water for 5 h. Measurements of electrolyte and Pi contents of the hydration medium wcre donc cvery hours. The results were expressed in percentages of relative intcgrity (% RI) of ccli membranes (Scherbakova et al, 1980), as RI (%) = 1-(T-0/T-C) lOO where T: total leakages at 85°C (ali membranes destroyed), 0 : leakages in response to osmotie stress, and C : lcakages of the control.…”

Section: Resultsmentioning

confidence: 99%

Effect of water deficit on cell permeability and on chloroplast integrity

Zuily-Fodil

Vazquez-Tello

Silva

1990

Bulletin de la Société Botanique de France. Actualités Botaniqu

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Summary.-The study of physiological mechanisms involved in drought tolerance at the cellular leve\ cou Id le ad tc the characterization of tolerance parameters useful in crop breedings. ln this study, we analyse in the case of Vigna and Phaseolus leaves, the affect of water deficit on cel\ permeability and chloroplast integrity.Chloroplasts were isolated from control and stressed plants. Thylakoid and stroma proteins were extracted from the isolated chloroplasts and analysed by electrophoresis.The results showed thal water deficit induced at the cel\ leval an increase in electrolyte and Pi leakages from leal dises treated with PEG and rehydrated, according tc the protoplasmic tolerance of the va rieti es. Concerning chloroplasts, we observed in response to drought : • an increase in the number of disorganized chloroplasts ; ·a decrease in the photosynthetic electron transfer ; • a decrease in prote in and chlorophy\1 contents of the chloroplasts ; ·modifications of prote in patterns of thylakoids ; • an inhibition of choloroplast protein synthesis.Most of these results correlate weil with the protoplasmic tolerance of the varieties which is also correlated with the behaviour of field grown plants in response ta water stress. Therefore, seme of these physiological and biochemical criteria could be used for the screening of varieties in plant breeding programs.Résumé.-La sécheresse est le facteur majeur limitant la productivité et la qualité des récoltes (Lawlor, 1 979). L'étude des mécanismes impliqués dans la tolérance à la sécheresse au niveau cellulaire devrait conduire à la caractérisation de marqueurs physiologiques de résistance dont l'utilisation pour l'amélioration des Fig. 1 et 2). L'intensité des afflux s'est avérée être bien corrélée avec la résistance des variétés en champs, ce qui a conduit à leur classification en fonction de leur résistance protoplasmique.© Société botanique de France 1990. ISSN 0181-1789 Published online 10 Jul 2014 116 ACTUALITES BOTANIQUES l'augmentation des afflux de Pi est due, en grande partie, à l'activité de phosphotases acides localisées dans les chloroplastes. Nous avons donc cherché à préciser l'effet du déficit hydrique sur ce compartiment cellulaire en analysant les chloroplastes isolés à partir de feuilles de plantes bien hydratées et de plantes soumises à une sécheresse contrôlée (Tableau 1). la mise au point d'une méthode d'isolement de chloroplastes associant l'utilisation d'inhibiteurs de l'activité protéolytique et un ajustement osmotique a permis d'obtenir 2 populations de chloroplastes : c. denses, intacts etc. légers dont l'enveloppe est lésée (Fig. 3). Nous avons montré que la sécheresse provoque une inhibition du transfert des électrons (réaction de Hill), une augmentation de la proportion en chloroplastes lésés (Fig. 3) et une diminution des teneurs en chlorophylle et en protéines dans les deux catégories de chloroplastes (Fig. 5 et 6).En ce qui concerne les protéines, l'effet du stress est très bien corrélé avec le degré de sensibilité protoplasmique des ...

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Modification of stress tolerance by dehydration pretreatment in winter rape hypocotyls

Cited by 18 publications

References 10 publications

Solute Leakage Resulting from Leaf Desiccation

Solute Leakage Resulting from Leaf Desiccation

Effect of water stress conditioning on the water relations, root growth capacity, and the nitrogen and non-structural carbohydrate concentration of Pinus halepensis Mill. (Aleppo pine) seedlings

Effect of water deficit on cell permeability and on chloroplast integrity

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