Modification of the conformational skin structure by treatment with liposomal formulations and its correlation to the penetration depth of aciclovir

Hasanovic, Amra; Winkler, Regina; Resch, Guenter P.; Valenta, Claudia

doi:10.1016/j.ejpb.2011.01.018

Cited by 46 publications

(17 citation statements)

References 33 publications

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“…The following bands were recorded: CH 2 asymmetric (2920 cm −1 ) and symmetric stretching vibration (2850 cm −1 ), amide I (C O) vibration (1640 cm −1 ) and amide II (C-N) vibration (1540 cm −1 ) and the CH 2 scissoring mode (between 1470 and 1460 cm −1 ). These bands are caused by the intercellular lipids and keratin inside the corneocytes of the skin and correlate well with values found in the literature (Hasanovic et al, 2011;Obata et al, 2010;Rodríguez et al, 2010). In Fig.…”

Section: Atr-ftirsupporting

confidence: 79%

“…Infrared spectra of impregnated porcine ear skin samples were obtained using a Tensor 27 FTIR instrument (Bruker Optics, Germany) with a Bio-ATR I tool and a photovoltaic MCT detector at the skin surface temperature of 32 • C. Porcine ear skin samples were treated as described in Hasanovic et al (2011). For the measurements, the skin samples were placed on the ZnSe ATR crystal with the stratum corneum facing down.…”

Section: Atr-ftir Spectroscopymentioning

confidence: 99%

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Optimisation of multiple W/O/W nanoemulsions for dermal delivery of aciclovir

Schwarz

Klang

Karall

et al. 2012

International Journal of Pharmaceutics

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Section: Atr-ftirsupporting

confidence: 79%

Section: Atr-ftir Spectroscopymentioning

confidence: 99%

Optimisation of multiple W/O/W nanoemulsions for dermal delivery of aciclovir

Schwarz

Klang

Karall

et al. 2012

International Journal of Pharmaceutics

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“…Attenuated total reflectance Fourier-transform infrared spectroscopy (ATR-FTIR) spectroscopic studies were performed to study the formulation effect on the biophysical properties of the stratum corneum (30). It has been already demonstrated that porcine skin is very close to human skin in structural and chemical properties and a very helpful model for the transdermal drug diffusion study in combination with FTIR (31).…”

Section: Discussionmentioning

confidence: 99%

Enhanced Topical Delivery of Finasteride Using Glyceryl Monooleate-Based Liquid Crystalline Nanoparticles Stabilized by Cremophor Surfactants

et al. 2013

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Abstract. The aim of this study was to investigate the capability of two surfactants, Cremophor RH 40 (RH) and Cremophor EL (EL), to prepare liquid crystalline nanoparticles (LCN) and to study its influence on the topical delivery of finasteride (FNS). FNS-loaded LCN was formulated with the two surfactants and characterized for size distribution, morphology, entrapment efficiency, in vitro drug release, and skin permeation/retention. Influence of FNS-loaded LCN on the conformational changes on porcine skin was also studied using attenuated total reflectance Fourier-transform infrared spectroscopy. Transmission electron microscopical image confirmed the formation of LCN. The average particle size of formulations was in the range of 165.1-208.6 and 153.7-243.0 nm, respectively. The formulations prepared with higher surfactant concentrations showed faster release and significantly increased skin permeation. Specifically, LCN prepared with RH 2.5% presented higher permeation flux (0.100± 0.005 μgcm −2 h −1 ) compared with lower concentration (0.029±0.007 μgcm −2 h −1 ). Typical spectral bands of lipid matrix of porcine skin were shifted to higher wavenumber, indicating increased degree of disorder of the lipid acyl chains which might cause fluidity increase of stratum corneum. Taken together, Cremophor surfactants exhibited a promising potential to stabilize the LCN and significantly augmented the skin permeation of FNS.

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“…Hair removal was performed on defrosted porcine ears (section 2.2. ); porcine ear skin was prepared for ATR-FTIR analysis as reported [16]: skin samples of 2 × 7 cm were cut and the skin was removed from the cartilage with a scalpel. For each type of epilated/depilated skin, at least 10 skin areas from 4 different ears were employed (2-3 experiments per ear).…”

Section: Atr-ftir Spectroscopymentioning

confidence: 99%

“…Biophysical measurement techniques were selected to investigate skin properties associated with barrier function, such as measurement of the transepidermal water loss (TEWL) and skin dielectric permittivity. Attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy was employed to investigate the effect of hair removal on SC constituents such as lipid matrix and proteins [15,16]. The SC thickness was determined by confocal Raman spectroscopy (CRS) [17][18][19] before and after hair removal.…”

Section: Introductionmentioning

confidence: 99%

Effect of Physical and Chemical Hair Removal Methods on Skin Barrier Function in vitro: Consequences for a Hydrophilic Model Permeant

Pany

Klang²,

Brunner

et al. 2018

Skin Pharmacol Physiol

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Background: Although very common in our society, the effect of hair removal on physiological skin parameters and on the ingress of applied chemicals has not been systematically investigated. Thus, as a first step, the aim of the present study was to elucidate the effect of hair removal through epilation (electric epilation, waxing) and depilation (dry and wet shaving, depilatory cream) on skin properties in vitro using the porcine ear model. Methods: Attenuated total reflection Fourier transform infrared spectroscopy, measurement of the transepidermal water loss (TEWL), visualization by capacitance-based contact imaging, confocal Raman spectroscopy (CRS), diffusion cell studies and tape stripping experiments were employed. Results: Increased TEWL and altered skin permittivity maps were observed. Decreased stratum corneum thickness was observed after waxing. Diffusion cell studies showed increased skin permeation especially in case of dry shaving, electric epilation and waxing. Conclusion: Considering CRS and diffusion cell data, a moderate if significant decrease in skin barrier function was found after hair removal by dry shaving (physical skin/material interaction) and epilation methods (plucking out the entire hair, for example, by electrical epilation and waxing). Subsequent experiments will include testing of different permeants covering a broad range of physicochemical properties in vitro and confirming our findings in vivo.

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Modification of the conformational skin structure by treatment with liposomal formulations and its correlation to the penetration depth of aciclovir

Cited by 46 publications

References 33 publications

Optimisation of multiple W/O/W nanoemulsions for dermal delivery of aciclovir

Optimisation of multiple W/O/W nanoemulsions for dermal delivery of aciclovir

Enhanced Topical Delivery of Finasteride Using Glyceryl Monooleate-Based Liquid Crystalline Nanoparticles Stabilized by Cremophor Surfactants

Effect of Physical and Chemical Hair Removal Methods on Skin Barrier Function in vitro: Consequences for a Hydrophilic Model Permeant

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