Modifying effects of δ-Aminolevulinate dehydratase polymorphism on blood lead levels and ALAD activity

Mani, Monica Shirley; Kunnathully, Vidya Satheesn; Rao, C. Mallikarjuna; Kabekkodu, Shama Prasada; Joshi, Manjunath B.; Dsouza, Herman Sunil

doi:10.1016/j.toxlet.2018.07.014

Cited by 22 publications

(12 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When these metals are released with the kiln, they spread over wide areas aided by the wind and finally settle on soil through which human exposure could ensue (Kolo et al, 2018). Indeed, exposure to Pb may disrupt cellular redox state, inhibit heme biosynthesis (Mani et al, 2018), and cause mitochondrial apoptosis (Jin et al, 2017). Cd may disrupt the metabolism of lipids by altering the levels of triacylglycerol (TAG), cholesterol (CHOL), and lipoproteins via the inhibition of lipogenic enzymes (Yang et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Bioaccumulation of heavy metals, lipid profiles, and antioxidant status of snails (Achatina achatina) around cement factory vicinities

et al. 2020

View full text Add to dashboard Cite

Some snails ( Achatina spp) can be used as a biosensor of heavy metal poisoning. This study thus estimated some heavy metal levels, antioxidant markers, and lipid profiles of snails handpicked around cement factory vicinities in Ogun State, Nigeria. Snails and soil samples were collected from Oke, Ewekoro, Papalanto, and Mowodani Imeko-Afon (control site). Lead (Pb), cadmium (Cd), and arsenic (As) levels were estimated in the soil, snail foot, hemolymph, and shell using Atomic Absorption Spectrophotometry. Triacylglycerol (TAG), phospholipids (PHOL), cholesterol (CHOL), malondialdehyde (MDA), and reduced glutathione (GSH) levels, as well as glutathione- S-transferase (GST), lactate dehydrogenase (LDH), and arylesterase (AR) activities in the hemolymph, were estimated spectrophotometrically. The snails collected from the Oke site had the highest foot Pb (274.66 ± 13.50 mg/g tissue), CHOL, TAG, PHOL levels, and GST activity when compared with other sites. Snails collected from Papa had the highest Cd levels (1.79 ± 0.74 mg/kg), As (1206 ± 18.87 mg/g tissue) in the foot, and LDH activity, while Ewekoro snails had highest MDA levels and AR activities but the lowest GSH levels. Additionally, there were negative correlations between the heavy metal levels and the activities of GST and AR as well as GSH levels, while positively correlating with LDH activity and MDA level. Workers and the general public around cement factories are at a greater risk of heavy metal-induced pathologies. More so, consumption of snails around these sites may be deleterious to health.

show abstract

Section: Introductionmentioning

confidence: 99%

Bioaccumulation of heavy metals, lipid profiles, and antioxidant status of snails (Achatina achatina) around cement factory vicinities

et al. 2020

View full text Add to dashboard Cite

show abstract

“…The present study showed a negative correlation between BLLs and ALAD activity, as previously reported. 7,18 Cigarette smoke and smokeless tobacco products are well known acquired factors for the elevation of BLLs. 40 WHO estimated that a smoker inhales typically about 2-6% of lead, which is equivalent to 1-5 mg lead after smoking 20 cigarettes per day.…”

Section: Discussionmentioning

confidence: 99%

“…ALAD codes for the enzyme d-aminolevulinate dehydratase, widely studied in association with lead toxicity. 6,18,[44][45][46][47][48] However, studies on the influence of VDR haplotypes and HFE polymorphisms on BLLs of occupationally exposed subjects are limited. In the present study, the mutant TT genotype of rs1544410, mutant AA genotype of rs7975232, and wild-type AA genotype of rs731236 polymorphisms presented a higher median BLL compared to other genotypes in the lead-exposed group.…”

Section: Discussionmentioning

confidence: 99%

Influence of VDR and HFE polymorphisms on blood lead levels of occupationally exposed workers

et al. 2020

Self Cite

View full text Add to dashboard Cite

Lead is a ubiquitous heavy metal toxin of significant public health concern. Every individual varies in their response to lead’s toxic effects due to underlying genetic variations in lead metabolizing enzymes or proteins distributed in the population. Earlier studies, including our lab, have attributed the influence of ALAD (δ-Aminolevulinate dehydratase) polymorphism on blood lead retention and ALAD activity. The present study aimed to investigate the influence of VDR (Vitamin D receptor) and HFE (Hemochromatosis) polymorphisms in modulating blood lead levels (BLLs) of occupationally exposed workers. 164 lead-exposed subjects involved in lead alloy manufacturing and battery breaking and recycling processes and 160 unexposed controls with BLLs below 10 µg/dL recruited in the study. Blood lead levels, along with a battery of biochemical assays and genotyping, were performed. Regression analysis revealed a negative influence of BLLs on ALAD activity ( p < 0.0001) and a positive influence on smokeless tobacco use ( p < 0.001) in lead-exposed subjects. A predicted haplotype of the three VDR polymorphisms computed from genotyping data revealed that T-A-A haplotype increased the BLLs by 0.93 units ( p ≤ 0.05) and C-C-A haplotype decreased the BLLs by 7.25 units ( p ≤ 0.05). Further analysis revealed that the wild-type CC genotype of HFE H63D presented a higher median BLL, indicating that variant C allele may have a role in increasing the concentration of lead. Hence, the polymorphism of genes associated with lead metabolism might aid in predicting genetic predisposition to lead and its associated effects.

show abstract

“…Lead (Pb) is commonly known as a toxic heavy metal and is widely distributed in the environment due to its non‐biodegradability (Palmer et al ., 2015). Meanwhile, its multiple affinities make it easy to enter the human body through the food chain and cause severe damages to the nervous system, the immune system and organs including livers, kidneys and lungs (Benbow, Aubrecht, Banker, Nettleton, & Aleo, 2010; Mani et al ., 2018; Zhang et al ., 2019). Furthermore, epidemiological and experimental studies indicated that Pb leads to oxidative damage to various biological macromolecules, influencing on necrosis and apoptosis, gene expression, damaging DNA repair, etc.…”

Section: Introductionmentioning

confidence: 99%

Deciphering the role of selenium‐enriched rice protein hydrolysates in the regulation of Pb²⁺‐induced cytotoxicity: an in vitro Caco‐2 cell model study

Zhu

Ding

Fang

et al. 2020

Int J of Food Sci Tech

View full text Add to dashboard Cite

Lead (Pb), a well-known toxic metal, has a potential of adverse effects on human health. Selenium (Se) can reduce the toxicity caused by heavy metals. In this study, we investigated the protection mechanism of the purified Se-enriched rice protein hydrolysates (SPHs-2) on the Pb 2+-induced cytotoxicity. A Caco-2 cell model was established to characterise the effect of SPHs-2 on Pb 2+ absorption in the simulated small intestine. Results showed that 1000 lg mL À1 of SPHs-2 treatment could significantly increase cell viability by 19.43% in Pb 2+-treated Caco-2 cells (P < 0.05). Furthermore, the absorption of Pb 2+ was successfully retarded by incorporating SPHs-2. Interestingly, selenomethionine (SeMet) was identified as a major Se species in SPHs-2 by HPLC-ICP-MS, and SeMet played a crucial role in regulating Pb 2+ intestinal absorption via forming complexes. In light of this, SPHs-2 may serve as a potential Pb 2+-chelating peptide for developing novel functional food and protecting human health.

show abstract

Modifying effects of δ-Aminolevulinate dehydratase polymorphism on blood lead levels and ALAD activity

Cited by 22 publications

References 19 publications

Bioaccumulation of heavy metals, lipid profiles, and antioxidant status of snails (Achatina achatina) around cement factory vicinities

Bioaccumulation of heavy metals, lipid profiles, and antioxidant status of snails (Achatina achatina) around cement factory vicinities

Influence of VDR and HFE polymorphisms on blood lead levels of occupationally exposed workers

Deciphering the role of selenium‐enriched rice protein hydrolysates in the regulation of Pb²⁺‐induced cytotoxicity: an in vitro Caco‐2 cell model study

Contact Info

Product

Resources

About

Modifying effects of δ-Aminolevulinate dehydratase polymorphism on blood lead levels and ALAD activity

Cited by 22 publications

References 19 publications

Bioaccumulation of heavy metals, lipid profiles, and antioxidant status of snails (Achatina achatina) around cement factory vicinities

Bioaccumulation of heavy metals, lipid profiles, and antioxidant status of snails (Achatina achatina) around cement factory vicinities

Influence of VDR and HFE polymorphisms on blood lead levels of occupationally exposed workers

Deciphering the role of selenium‐enriched rice protein hydrolysates in the regulation of Pb2+‐induced cytotoxicity: an in vitro Caco‐2 cell model study

Contact Info

Product

Resources

About

Deciphering the role of selenium‐enriched rice protein hydrolysates in the regulation of Pb²⁺‐induced cytotoxicity: an in vitro Caco‐2 cell model study