Modulating Effects of Heparin Preparations on the DNA Synthesis Response of Human Peripheral Blood T Lymphocytes Activated by Mercuric Chloride and Nickel Sulfate

Nordlind, Klas

doi:10.1159/000234416

Cited by 3 publications

(1 citation statement)

References 11 publications

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“…Mercuric chloride is a polyclonal activator of hu man T lymphocytes [1][2][3], and nickel sulfate stimu lates T lymphocytes from nickel-allergic subjects [4], The stimulation by these metal salts may be modu lated by various factors, e.g., neuropeptides [5,6] and heparin preparations [7],…”

Section: Introductionmentioning

confidence: 99%

Different Modulating Effects of the Monoamines Adrenaline, Noradrenaline, and Serotonin on the DNA Synthesis Response of Human Peripheral Blood T Lymphocytes Activated by Mercuric Chloride and Nickel Sulfate

Nordlind

Sundström²

1988

Int Arch Allergy Immunol

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The influence of adrenaline, noradrenaline, and serotonin (5-hydroxytryptamine) was tested on the proliferative response of peripheral blood T lymphocytes of nickel-allergic subjects to mercuric chloride and nickel sulfate. With addition of the monoamines 1 h after mercuric chloride, there was a marked suppression with all the substances at 10^––⁴M, while adrenaline also inhibited down to a concentration of 10^––5M and serotonin down to 10^––7M. At 3 days after mercuric chloride, adrenaline inhibited at 10^––4M and serotonin also down to a concentration of 10^––5M. With addition at 1 h after nickel sulfate, there was an inhibition by all the monoamines at 10^––4M, while, in contrast, adrenaline at 10^––6 to 10^––8M and serotonin at 10^––5 to 10^––6M gave a stimulation of the response. These findings point at different modulating effects on the proliferative response induced by a polyclonal activator such as mercuric chloride and a monoclonal activator such as nickel sulfate.

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Section: Introductionmentioning

confidence: 99%

Different Modulating Effects of the Monoamines Adrenaline, Noradrenaline, and Serotonin on the DNA Synthesis Response of Human Peripheral Blood T Lymphocytes Activated by Mercuric Chloride and Nickel Sulfate

Nordlind

Sundström²

1988

Int Arch Allergy Immunol

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Miscellaneous treatments, II: niacin and heparin: unapproved uses, dosages, or indications

Wolf

Orion

Matz

et al. 2002

Clinics in Dermatology

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Effect of Salbutamol, Fenoterol, and Sodium Cromoglycate on the Release of Heparin from Sensitized Human Lung Fragments Challenged with Dermatophagoides pteronyssinus Allergen

Green

Konnaris²,

Woolcock³

1993

Am J Respir Cell Mol Biol

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Heparin from degranulating mast cells influences a wide range of cellular and humoral reactions associated with allergic inflammation and asthma. Agents that inhibit mast cell degranulation may therefore compromise the moderating effects of heparin in the tissues and result in worsening inflammation and other associated pathology. This study measures heparin release from allergen-challenged human lung tissue and compares the effect of the mast cell stabilizing beta 2-agonists, salbutamol and fenoterol, and a non-beta 2-agonist, sodium cromoglycate, on the release of heparin. Pieces of lung tissue 2 to 3 mm3 were sensitized with high titer Dermatoaphagoides pteronyssinus-specific IgE serum and challenged with D. pteronyssinus allergen, with and without prior addition of salbutamol, fenoterol, or sodium cromoglycate. Dextran sulfate was added to the mixture to prevent the binding of heparin to tissue proteins. Heparin was released together with histamine after challenge. The mean and 95% confidence interval of prechallenge and postchallenge heparin concentrations in the lung tissue filtrates were 0.10 IU/ml (0.07, 0.12) and 0.24 IU/ml (0.17, 0.30), respectively (P < 0.001). Addition of the beta 2-agonists produced a mean inhibition of released heparin of 71% (50, 92), and 73% (55, 91), respectively. Sodium cromoglycate gave a 35% (20, 51) inhibition that was significantly less than that produced by the beta 2-agonists (P < 0.01). The beta 2-agonists salbutamol and fenoterol strongly inhibited heparin release from mast cells. The therapeutic use of mast cell stabilizing agents may therefore be potentially detrimental to the control of allergic inflammation and other associated pathologies.

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Modulating Effects of Heparin Preparations on the DNA Synthesis Response of Human Peripheral Blood T Lymphocytes Activated by Mercuric Chloride and Nickel Sulfate

Cited by 3 publications

References 11 publications

Different Modulating Effects of the Monoamines Adrenaline, Noradrenaline, and Serotonin on the DNA Synthesis Response of Human Peripheral Blood T Lymphocytes Activated by Mercuric Chloride and Nickel Sulfate

Different Modulating Effects of the Monoamines Adrenaline, Noradrenaline, and Serotonin on the DNA Synthesis Response of Human Peripheral Blood T Lymphocytes Activated by Mercuric Chloride and Nickel Sulfate

Miscellaneous treatments, II: niacin and heparin: unapproved uses, dosages, or indications

Effect of Salbutamol, Fenoterol, and Sodium Cromoglycate on the Release of Heparin from Sensitized Human Lung Fragments Challenged with Dermatophagoides pteronyssinus Allergen

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