Modulation of Aging in Yeast Saccharomyces cerevisiae by Roselle Petal Extract (<i>Hibiscus sabdariffa</i> L.)

Sarima,; Astuti, Rika Indri; Meryandini, Anja

doi:10.3844/ajbbsp.2019.23.32

Cited by 11 publications

(15 citation statements)

References 33 publications

(38 reference statements)

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“…Our data suggest that clove leaf extract could promote oxidative stress tolerance in yeast cells. Other extracts that are capable of inducing oxidative stress tolerance response in yeast are green tea extract [29], Red chicory leaf [30], Tinospora cordifolia [31], and roselle petal [32].…”

Section: Resultsmentioning

confidence: 99%

Effect of Ethanol-Derived Clove Leaf Extract on the Oxidative Stress Response in Yeast Schizosaccharomyces pombe

Fauzya

Astuti

Mubarik

2019

International Journal of Microbiology

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Compared to the widely explored antioxidant activity from the clove bud extract, less data are available regarding the potential pharmacological use of clove leaves. Our study aimed to explore the antioxidant activity of clove leaves extract in the cellular level. Thus, we used the yeast Schizosaccharomyces pombe as model organisms. Our data indicate that, following extract treatment (100 ppm), the viability of the stationary phase cells of S. pombe was higher than without extract and that of calorie restriction treatments. 100 ppm extract treatment also increased cell viability against H2O2-induced oxidative stress. Those data indicate that the extract could promote oxidative stress tolerance response in yeast cells, which occurred either during the stationary phase or due to exogenous exposure. Higher dose of extract (500 ppm) showed opposite effects, as cell viability was lower than that without treatment. Analysis toward the mitochondrial activity revealed that the extract did not induce mitochondrial activity unlike the calorie restriction treatment. Based on our data, clove leaf extract promotes oxidative stress tolerance response in the yeast S. pombe, independent to that mitochondrial adaptive ROS signaling which commonly occurs in calorie restriction-induced oxidative stress tolerance response.

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Section: Resultsmentioning

confidence: 99%

Effect of Ethanol-Derived Clove Leaf Extract on the Oxidative Stress Response in Yeast Schizosaccharomyces pombe

Fauzya

Astuti

Mubarik

2019

International Journal of Microbiology

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“…Our data indicated that the plant extracts/fractions induced the capability of yeast cells to cope with strong oxidative stress conditions. Previous studies revealed the potential of various extracts of plants, including rosella and clove, in promoting yeast viability in oxidative stress conditions [ 25 , 33 ]. The particular extract with such antioxidant activities may be developed as an antiaging agent.…”

Section: Resultsmentioning

confidence: 99%

Antiaging and Antioxidant Bioactivities of Asteraceae Plant Fractions on the Cellular Functions of the Yeast Schizosaccharomyces pombe

Astuti

Prastya

Batubara

et al. 2021

Advances in Pharmacological and Pharmaceutical Sciences

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Research on antioxidants has been gaining worldwide attention because of their essential applications for medicinal purposes. In this study, we conducted bioprospecting of six Asteraceae plants as the source of antiaging and antioxidant agents. Water and chloroform fractions from Ageratum conyzoides L., Dichrocephala integrifolia (L.f.) Kuntze, Galinsoga parviflora (Cav.), Mikania micrantha Kunth, Sphagneticola trilobata (L.) Pruski, and Synedrella nodiflora L. were collected and assayed for their in vitro antioxidant activities and potential antiaging properties using the yeast Schizosaccharomyces pombe as the model organism. Based on the in vitro assay, the water fractions of S. trilobata showed a strong antioxidant activity. Interestingly, all treatment solutions promoted the stress tolerance phenotype of S. pombe to strong H2O2-induced oxidative stress conditions. Moreover, compared with the treatments without plant extract/fraction, all extract and fraction treatments, except the chloroform fractions of A. conyzoides, promoted yeast cell longevity. Strong induction of mitochondria activity was found following the treatments with the extracts and fractions of S. nodiflora, D. integrifolia, and M. micrantha and likely mimicked the calorie restriction-induced lifespan. Interestingly, S. nodiflora water fractions significantly upregulated the mRNA transcripts of the Pap1-mediated core environmental stress response, namely, ctt1 gene in S. pombe. These data indicated that the fractions of Asteraceae plants had potential antioxidant and antiaging activities through various cellular modulations. S. nodiflora water fraction has been shown to have antioxidant and antiaging activities in S. pombe, by modulating stress tolerance response, inducing mitochondrial activity, and increasing the ctt1 gene expression. Compounds analysis identified that S. nodiflora water fraction contained some primarily compounds including oxyphyllacinol, valine, and sugiol.

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“…Low glucose in the positive control is considered as calorie restriction (CR) conditions. Such CR condition will induce yeast cellular mechanisms including mitochondrial activity response to oxidative stress and autophagy, which contribute to the yeast lifespan extension (Roux et al, 2010;Sarima et al, 2019). Each culture was incubated for 11 th -days where a spot test was conducted at days 7 and 11 of incubations.…”

Section: Aging Assaymentioning

confidence: 99%

“…The procedure was performed according to the previous study by using Rhodamine B as a probe (Sarima et al, 2019). The yeast was incubated in liquid YES medium supplemented with phytoextracts of X. granatum and Xyloccensins K at the best concentration during the aging assay.…”

Section: Active Mitochondrial Stainingmentioning

confidence: 99%

Anti-Aging Activity of Xylocarpus Granatum Phytoextracts and Xyloccensins K Compound

et al. 2021

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Cellular aging is promoted by the deleterious effect of free radicals. This can be lowered by antioxidant treatments. Xylocarpus granatum and its compound, Xyloccensins K have been reported to have antioxidant activity but there have been no reports of antioxidant and anti-aging activities at the cellular level. Thus, the aim of this study to investigate the antioxidant and anti-aging properties of X. granatum-derived extract and Xyloccensins K at a cellular level in yeast Schizosaccharomyces pombe. Four vegetative and three generative parts of X. granatum organs including root, stem, leaf, twig, seed, flesh of fruit, and peel of fruit were extracted using 70% ethanol by the maceration method. Whereas, Xyloccensins K was obtained from seed of X. granatum. The samples tested, other than peel of fruit, prolonged cell longevity in lower concentration as compared to that without phytoextracts treatment. Also, our data indicate that all samples could promote oxidative stress tolerance phenotype, as yeast was capable of dealing with H2O2-induced oxidative stress treatment at 1, 2, and 3 mM H2O2 with the best phenotypes by the administration of twig extracts. Most of the phytoextracts showed an increase in mitochondrial activity, except that of seed extract. The result showed the administration of Xyloccensins K compound did not increase the expression of transcriptional factors of oxidative stress response gene cluster, sty1 and pap1. We suggest that the Xyloccensins K compound acts as direct Reactive Oxygen Species (ROS) scavenger. Thus further study in elucidating the phenomenon of longevity-induced X. granatum extract is required.

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Modulation of Aging in Yeast Saccharomyces cerevisiae by Roselle Petal Extract (Hibiscus sabdariffa L.)

Cited by 11 publications

References 33 publications

Effect of Ethanol-Derived Clove Leaf Extract on the Oxidative Stress Response in Yeast Schizosaccharomyces pombe

Effect of Ethanol-Derived Clove Leaf Extract on the Oxidative Stress Response in Yeast Schizosaccharomyces pombe

Antiaging and Antioxidant Bioactivities of Asteraceae Plant Fractions on the Cellular Functions of the Yeast Schizosaccharomyces pombe

Anti-Aging Activity of Xylocarpus Granatum Phytoextracts and Xyloccensins K Compound

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