Modulation of antibiotic resistance in bacterial pathogens by oleanolic acid and ursolic acid

Kurek, Anna; Nadkowska, Paulina; Pliszka, Sylwia; Wolska, Krystyna I.

doi:10.1016/j.phymed.2011.12.009

Cited by 89 publications

(67 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The antibacterial and anti- Table 1 Minimum inhibitory (MIC) and fungicidal concentration (MFC) of hexane extract of F. drupacea and 5-O-methyllatifolin (3), oleanolic acid (4), epifriedelanol (5), friedelin (6), epilupeol acetate (7) Table 2 Minimum inhibitory (MIC) and bactericidal concentration (MBC) of hexane extract of F. drupacea and 5-O-methyllatifolin (3), oleanolic acid (4), epifriedelanol (5), friedelin (6), epilupeol acetate (7) fungal of triterpenes found in this study such as compound 7 had not been studied against collection wide range of human and plant pathogenic microorganisms. In recent studies, compound 4 showed antibacterial activities against L. monocytogenes and S. aureus (Kurek et al, 2010(Kurek et al, , 2012 and plant pathogenic fungi (Zhao et al, 2011), confirming the antibacterial activities found in this study. Our study, however, complements previous studies in that it reveals some antifungal activities of compound 4 against human pathogenic fungi.…”

Section: Resultssupporting

confidence: 89%

Antifungal, antibacterial and anticancer activities of Ficus drupacea L. stem bark extract and biologically active isolated compounds

Yessoufou

Elansary

Mahmoud

et al. 2015

Industrial Crops and Products

View full text Add to dashboard Cite

Section: Resultssupporting

confidence: 89%

Antifungal, antibacterial and anticancer activities of Ficus drupacea L. stem bark extract and biologically active isolated compounds

Yessoufou

Elansary

Mahmoud

et al. 2015

Industrial Crops and Products

View full text Add to dashboard Cite

“…This is quite likely because cuticular triterpenes are thought to play a role in plant defense, which signifies their accumulation in these organs (González-Coloma et al, 2011). Although the biological role of the pentacyclic triterpenes has not been clearly established, the potential insecticidal and antimicrobial activities of the pentacyclic triterpenes suggest a protective role against pests and pathogens (González- Coloma et al, 2011;Kurek et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

“…Ursane-type and oleanane-type pentacyclic triterpenes (C30 compounds) are known for a variety of pharmaceutical properties, including anti-inflammatory, hepatoprotective, antitumor, antihyperlipidemic, antiulcer, and antimicrobial benefits (Kunkel et al, 2011;Wilkinson et al, 2011;Kurek et al, 2012;da Silva Ferreira et al, 2013;Kong et al, 2013). However, there are limited data on the biosynthesis of these secondary metabolites in plants (Brendolise et al, 2011;Huang et al, 2012;Yu et al, 2013).…”

mentioning

confidence: 99%

Methyl Jasmonate-Elicited Transcriptional Responses and Pentacyclic Triterpene Biosynthesis in Sweet Basil

et al. 2013

View full text Add to dashboard Cite

Sweet basil (Ocimum basilicum) is well known for its diverse pharmacological properties and has been widely used in traditional medicine for the treatment of various ailments. Although a variety of secondary metabolites with potent biological activities are identified, our understanding of the biosynthetic pathways that produce them has remained largely incomplete. We studied transcriptional changes in sweet basil after methyl jasmonate (MeJA) treatment, which is considered an elicitor of secondary metabolites, and identified 388 candidate MeJA-responsive unique transcripts. Transcript analysis suggests that in addition to controlling its own biosynthesis and stress responses, MeJA up-regulates transcripts of the various secondary metabolic pathways, including terpenoids and phenylpropanoids/flavonoids. Furthermore, combined transcript and metabolite analysis revealed MeJA-induced biosynthesis of the medicinally important ursane-type and oleanane-type pentacyclic triterpenes. Two MeJAresponsive oxidosqualene cyclases (ObAS1 and ObAS2) that encode for 761-and 765-amino acid proteins, respectively, were identified and characterized. Functional expressions of ObAS1 and ObAS2 in Saccharomyces cerevisiae led to the production of b-amyrin and a-amyrin, the direct precursors of oleanane-type and ursane-type pentacyclic triterpenes, respectively. ObAS1 was identified as a b-amyrin synthase, whereas ObAS2 was a mixed amyrin synthase that produced both a-amyrin and b-amyrin but had a product preference for a-amyrin. Moreover, transcript and metabolite analysis shed light on the spatiotemporal regulation of pentacyclic triterpene biosynthesis in sweet basil. Taken together, these results will be helpful in elucidating the secondary metabolic pathways of sweet basil and developing metabolic engineering strategies for enhanced production of pentacyclic triterpenes.

show abstract

“…Nevertheless, probably by applying the combination in a preliminary stage of bacteria adhesion/biofilm formation, the combinations would be more effective, which would explain the potentiation observed. Kurek, et al (2012) 40 also found synergistic antibacterial effects of oleanolic acid in combination with ampicillin against biofilms of S. aureus and S. epidermidis, and with oxacillin against biofilms of L. monocytogenes, S. epidermis and S. aureus. Ursolic acid was found to inhibit biofilm formation of MRSA by reducing amino acids metabolism and expression of adhesins, 46 to induce genes related to chemotaxis, mobility and heat shock response, and to repress genes that have functions in cysteine synthesis and sulfur metabolism.…”

Section: Fractionation Of Active Extract Of Buxus Sempervirensmentioning

confidence: 96%

Antibiotic adjuvants from Buxus sempervirens to promote effective treatment of drug-resistant Staphylococcus aureus biofilms

et al. 2016

View full text Add to dashboard Cite

Plants have been long scrutinized in the quest for new antibiotics, but no strong antibiotic molecule was ever found. Evidences exist that most phytochemicals have a regulatory or adjuvant effect on other antibacterial compounds, thus promoting a greater therapeutic effect. The current study assessed twenty-eight plants from different families for their antibacterial activity and as adjuvants in antibiotic therapy against Staphylococcus aureus, including methicillin-resistant S. , respectively. Non-antibacterial plants were assessed in combination with ampicillin, oxacillin, ciprofloxacin, erythromycin and tetracycline by a modified disc diffusion test. Methanolic extracts of Acacia dealbata, Prunus spp. plants, Centaurea nigra, Eupatorium cannabium and Buxus sempervirens showed a potentiating effect mostly of ciprofloxacin, erythromycin and tetracycline. B. sempervirens was selected for its potentiating activity and applied against S. aureus biofilms. B.sempervirens (1 g L -1 ) was able to cause an 88% reduction of S. aureus within 1 h exposure. Further phytochemical investigation of B. sempervirens allowed to identify betulinic acid as a major component, together with other triterpenoids. Betulinic acid and other common terpernoids -lupeol, betulin, hederagenin, ursolic acid and oleanolic acid, were tested for antibacterial and antibiotic-potentiating activities. Among the tested compounds, oleanolic acid and ursolic acid -were highlighted, showing MIC of 62.5 and 15.6 mg L -1 , respectively, against S. aureus. Additionally, oleanolic acid showed synergism when combined with tetracycline and erythromycin and caused biofilm reductions of 70, 81 and 85% when applied at ½ MIC, MIC and 2 × MIC, respectively. IntroductionTwo major circumstances have accentuated the quest for new antibacterial agents and alternative therapies in the last decades. Primarily, because microbes, due to their incredible and innate adaptability, seem to have at least equal chances for survival as scientists and pharmaceutical industries develop methods to kill them. 3 Multidrug-resistant (MDR) bacteria are responsible for a large number of nosocomial but also community-acquired infections and are spreading all over the globe. 4 Additionally, the limitation of our current arsenal of effective antibiotics accompanied by the lack of new antimicrobial alternatives are prompting the beginning of the "post-antibiotic era", which threats all the achievements of modern medicine.Since the beginning of mankind plants were undoubtedly the most important source of therapeutic remedies with an enormous range of applications. The earliest records of natural products were depicted from Mesopotamia (2600 B.C.) and included oils from cypress (Cupressus sempervirens) and myrrh (Commiphora species), which are still used today to treat coughs, colds and inflammation. 5 Many plant extracts and their phytochemical constituents are known to have antimicrobial activities. 6 However, it can be rapidly established that this effort of finding individual active anti...

show abstract

Modulation of antibiotic resistance in bacterial pathogens by oleanolic acid and ursolic acid

Cited by 89 publications

References 29 publications

Antifungal, antibacterial and anticancer activities of Ficus drupacea L. stem bark extract and biologically active isolated compounds

Antifungal, antibacterial and anticancer activities of Ficus drupacea L. stem bark extract and biologically active isolated compounds

Methyl Jasmonate-Elicited Transcriptional Responses and Pentacyclic Triterpene Biosynthesis in Sweet Basil

Antibiotic adjuvants from Buxus sempervirens to promote effective treatment of drug-resistant Staphylococcus aureus biofilms

Contact Info

Product

Resources

About