Modulation of (Na,K)-ATPase activity by membrane fatty acid composition: therapeutic implications in human hypertension

Rodrigo, Ramón; Miranda-Merchak, Andrés; Grau, Rodrigo Valenzuela; Bachler, Juan P; Vergara, Leonardo

doi:10.3109/10641963.2013.783048

Cited by 29 publications

(18 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The comparison of linear regression curves by one-way ANOVA showed significant differences between the slopes (p ≤ 0.0001). These results are in agreement with previous studies, showing that the unsaturation index (UI) of membrane phospholipids is one of the major factors influencing membrane fluidity [21][22][23] In agreement with previous studies [34], fatty acid analysis of HMEC, enriched with EPA and DHA, revealed an increase of the ratio between saturated and polyunsaturated fatty acids, and a consequent increase in the concentration of double bonds in membrane phospholipids (unsaturation index, UI). As expected and depicted in Figure 2C, at each dose of n-3 LC-PUFA added to cell culture medium, DHA (six double bonds) determined a greater increase of UI than EPA (five double bonds).…”

Section: Effects Of N-3 Lc-pufa Incorporation On Membrane Fluidity Insupporting

confidence: 93%

Concentration-Dependent Effects of N-3 Long-Chain Fatty Acids on Na,K-ATPase Activity in Human Endothelial Cells

et al. 2019

View full text Add to dashboard Cite

N-3 eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) seem to prevent endothelial dysfunction, a crucial step in atherogenesis, by modulating the levels of vasoactive molecules and by influencing Na,K-ATPase activity of vascular myocytes. The activity of endothelial Na,K-ATPase controls the ionic homeostasis of the neighboring cells, as well as cell function. However, controversy exists with respect to the vascular protective effect of EPA and DHA. We argue that this dispute might be due to the use of different concentrations of EPA and DHA in different studies. Therefore, this study was designed to define an optimal concentration of EPA and DHA to investigate endothelial function. For this purpose, human endothelial cells were exposed for 24 h to different concentrations of DHA or EPA (0–20 μM) to study membrane fluidity, peroxidation potential and Na,K-ATPase activity. EPA and DHA were linearly incorporated and this incorporation was mirrored by the linear increase of unsaturation index, membrane fluidity, and peroxidation potential. Na,K-ATPase activity peaked at 3.75 μM of EPA and DHA and then gradually decreased. It is noteworthy that DHA effects were always more pronounced than EPA. Concluding, low concentrations of EPA and DHA minimize peroxidation sensitivity and optimize Na,K-ATPase activity.

show abstract

Section: Effects Of N-3 Lc-pufa Incorporation On Membrane Fluidity Insupporting

confidence: 93%

Concentration-Dependent Effects of N-3 Long-Chain Fatty Acids on Na,K-ATPase Activity in Human Endothelial Cells

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Regarding our second question, and based on the results of binomial logistic regression analysis, the GSSG/GSH ratio was the most significant predictive factor among OS parameters for the development of BRONJ. This ratio can be used to measure redox status , and some authors have confirmed that it is an indicator of systemic redox status . The transformation of GSH into GSSG involves the transformation of a hydrogen peroxide molecule into a water molecule .…”

Section: Discussionmentioning

confidence: 98%

Oxidative stress in bisphosphonate‐related osteonecrosis of the jaws

Bagán

Sáez

Tormos

et al. 2014

J Oral Pathology Medicine

View full text Add to dashboard Cite

show abstract

“…Moreover, in the groups receiving chronic HFD, both stressed or not, the activity of Na + ,K + -ATPase was similar to the control group. It is known that modifications in the membrane fatty acid profile will translate into effects in Na + ,K + -ATPase activity (Rodrigo et al, 2014). It is possible that a chronic administration of a HFD could be causing some alteration in membrane fluidity, changing the activity of this pump.…”

Section: Discussionmentioning

confidence: 99%

Early life adversities or high fat diet intake reduce cognitive function and alter BDNF signaling in adult rats: Interplay of these factors changes these effects

Arcego

Krolow

Lampert

et al. 2016

Intl J of Devlp Neuroscience

View full text Add to dashboard Cite

Environmental factors, like early exposure to stressors or high caloric diets, can alter the early programming of central nervous system, leading to long-term effects on cognitive function, increased vulnerability to cognitive decline and development of psychopathologies later in life. The interaction between these factors and their combined effects on brain structure and function are still not completely understood. In this study, we evaluated long-term effects of social isolation in the prepubertal period, with or without chronic high fat diet access, on memory and on neurochemical markers in the prefrontal cortex of rats. We observed that early social isolation led to impairment in short-term and working memory in adulthood, and to reductions of Na(+),K(+)-ATPase activity and the immunocontent of phospho-AKT, in prefrontal cortex. Chronic exposure to a high fat diet impaired short-term memory (object recognition), and decreased BDNF levels in that same brain area. Remarkably, the association of social isolation with chronic high fat diet rescued the memory impairment on the object recognition test, as well as the changes in BDNF levels, Na(+),K(+)-ATPase activity, MAPK, AKT and phospho-AKT to levels similar to the control-chow group. In summary, these findings showed that a brief social isolation period and access to a high fat diet during a sensitive developmental period might cause memory deficits in adulthood. On the other hand, the interplay between isolation and high fat diet access caused a different brain programming, preventing some of the effects observed when these factors are separately applied.

show abstract

Modulation of (Na,K)-ATPase activity by membrane fatty acid composition: therapeutic implications in human hypertension

Cited by 29 publications

References 62 publications

Concentration-Dependent Effects of N-3 Long-Chain Fatty Acids on Na,K-ATPase Activity in Human Endothelial Cells

Concentration-Dependent Effects of N-3 Long-Chain Fatty Acids on Na,K-ATPase Activity in Human Endothelial Cells

Oxidative stress in bisphosphonate‐related osteonecrosis of the jaws

Early life adversities or high fat diet intake reduce cognitive function and alter BDNF signaling in adult rats: Interplay of these factors changes these effects

Contact Info

Product

Resources

About