Modulation of Oxidative Stress by Functionalized Fullerene Materials in the Lung Tissues of Female C57/BL Mice with a Metastatic Lewis Lung Carcinoma

Abstract

Oxidative stress is considered to be one of the important mechanisms involved in carcinogenesis. To investigate the effect of [Gd@C82(OH)22]n and [C60(OH)20]n nanoparticles on the oxidative stress in the tumor-bearing mice, several antioxidative enzymes and antioxidants were tested for mice with or without tumor inoculation. Transplanted tumors were grown in mice by subcutaneous inoculation of a metastatic Lewis lung carcinoma in female C57/BL mice. More importantly, the tumor cells can metastasize into the normal lung tissues gradually. Therefore, in present paper, the activities of copper-zinc superoxide dismutase (CuZn-SOD), glutathione peroxidase (GSH-Px), glutathione S-transferase (GST), as well as the levels of reduced glutathione (GSH) and malondialdehyde (MDA) in the tumor-invaded lung tissues of the tumor-bearing mice were compared to the nomal lung tissues of normal mice. After treatment with nanoparticles, the activities of GSH-Px and GST and other parameters related to the oxidative stress were downregulated and tended closely to the normal levels. Pulmonary histopathological results also showed that two different types of water-soluble fullerenes can prevent lungs from inflammatory lesion and tumor invasion. These findings indicate two different types of water-soluble fullerenes materials can downregulate the oxidative stress status by scavenging excessive free radicals and inhibiting the lipid peroxidation in tumor-bearing mice, which can partly explain their protective roles on the pulmonary oxidative-damage induced by the tumor metastasis to lung tissues.

Biological characterizations of [Gd@C82(OH)22]nnanoparticles as fullerene derivatives for cancer therapy

“…The antioxidant effect may contribute to the efficiency of [Gd@C 82 (OH) 22 ] n nanoparticles in inhibiting tumor growth and reducing oxidative damage in the livers of tumor-bearing mice. 9,29,30 …”

Sodium Tanshinone IIA Sulfonate Attenuates Tumor Oxidative Stress and Promotes Apoptosis in an Intermittent Hypoxia Mouse Model

“…Briefly, the frozen tumor tissue samples were homogenized with a 5-mL glass Potter-Elvehjem homogenizer in ice-cold PBS (10% Wt/Vol). 22,28 After lysis for 15 minutes on ice, the homogenates were centrifuged and the supernatants were obtained for further investigation. Malondialdehyde concentration in the homogenates was determined spectrophotometrically by measuring the presence of thiobarbituric acid reactive substances (utilizing the principle of the thiobarbituric acid colorimetric method).…”

A Novel Drug Design Strategy: An Inspiration from Encaging Tumor by Metallofullerenol Gd@C82(OH)22

“…Gd@C 82 (OH) 22 has been intensively investigated ranging from chemical synthesis, property investigation, biological effects and the potential safety concerns [36,38,39]. It has been demonstrated to inhibit tumor growth and metastasis with high efficiency and low toxicity in vitro and in vivo [40,41,42].…”