Modulation of the Gut Microbiome and Obesity Biomarkers by Lactobacillus Plantarum KC28 in a Diet-Induced Obesity Murine Model

Huang, Eunchong; Kim, Seul-Ki; Park, Haryung; Park, Soyoung; Ji, Yosep; Todorov, Svetoslav Dimitrov; Lim, Sang-Dong; Holzapfel, Wilhelm H.

doi:10.1007/s12602-020-09720-0

Cited by 12 publications

(4 citation statements)

References 69 publications

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“…Stabilization of the intestinal barrier accompanied by a reduction in the adipose tissue mass was observed with the administration of an LPKC28 strain [39]. Here, a moderate increase in plasma LPS and a reduction in the intestinal ZO-1 levels were observed in the HFD group compared with the ND group.…”

Section: Discussionmentioning

confidence: 79%

<i>Lactiplantibacillus plantarum</i> 06CC2 upregulates intestinal ZO-1 protein and bile acid metabolism in Balb/c mice fed high-fat diet

YAMASAKI

Miyamoto

Ogawa

et al. 2024

Bioscience of Microbiota, Food and Health

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The effects of Lactiplantibacillus plantarum 06CC2 (LP06CC2), which was isolated from a Mongolian dairy product, on lipid metabolism and intestinal tight junction-related proteins in Balb/c mice fed a high-fat diet (HFD) were evaluated. The mice were fed the HFD for eight weeks, and the plasma and hepatic lipid parameters, as well as the intestinal tight junction-related factors, were evaluated. LP06CC2 slightly reduced the adipose tissue mass. Further, it dose-dependently decreased plasma total cholesterol (TC). The HFD tended to increase the plasma level of endotoxin and suppressed intestinal ZO-1 expression, whereas a low LP06CC2 dose increased ZO-1 expression and tended to reduce the plasma lipopolysaccharide level. Furthermore, a low LP06CC2 dose facilitated a moderate accumulation of Lactobacillales, a significant decrease in Clostridium cluster IV, and an increase in Clostridium cluster XVIII. The results obtained from analyzing the bile acids (BAs) in feces and cecum contents exhibited a decreasing trend for secondary and conjugated BAs in the low LP06CC2-dose group. Moreover, a high LP06CC2 dose caused excess accumulation of Lactobacillales and failed to increase intestinal ZO-1 and occludin expression, while the fecal butyrate level increased dose dependently in the LP06CC2-fed mice. Finally, an appropriate LP06CC2 dose protected the intestinal barrier function from the HFD and modulated BA metabolism.

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Section: Discussionmentioning

confidence: 79%

<i>Lactiplantibacillus plantarum</i> 06CC2 upregulates intestinal ZO-1 protein and bile acid metabolism in Balb/c mice fed high-fat diet

YAMASAKI

Miyamoto

Ogawa

et al. 2024

Bioscience of Microbiota, Food and Health

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“…The gut microbiota produces a variety of compounds that play an important role in modulating distal organ activity, and the liver is located downstream of the intestine, indicating the significance of the gut–liver axis [ 23 ]. As a promising anti-obesity candidate, microbiota regulation and its anti-obesity effects on L. plantarum have been elucidated by different researchers [ 16 , 24 , 25 ]. In our previous study, L. plantarum FRT10 intervention reduced the extent and features of obesity in HFD-induced obese mice by modulating the structure of gut microbiota [ 11 ].…”

Section: Discussionmentioning

confidence: 99%

Metabolomic Characteristics of Liver and Cecum Contents in High-Fat-Diet-Induced Obese Mice Intervened with Lactobacillus plantarum FRT10

Cai

Song

et al. 2022

Foods

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Obesity has become a major social problem related to health and quality of life. Our previous work demonstrated that Lactobacillus plantarum FRT10 alleviated obesity in high-fat diet (HFD)-fed mice by alleviating gut dysbiosis. However, the underlying functions of FRT10 in regulating liver and cecum contents metabolism remain unknown. Liver and cecum contents metabonomics combined with pathway analysis based on ultraperformance liquid chromatography-quadrupole-time-of-flight mass spectrometry (UHPLC-Q-TOF/MS) were performed to evaluate the alterations of metabolic profiles between obese control mice and obese mice in FRT10-treated groups. The orthogonal partial least squares discriminant analysis (OPLS-DA) score plots showed that there were significant differences in cecum contents and liver markers between experimental groups. In total, 26 potential biomarkers were identified in the liver and 15 in cecum contents that could explain the effect of FRT10 addition in HFD-fed mice. In addition, gut–liver axis analysis indicated that there was a strong correlation between cecum contents metabolites and hepatic metabolites. The mechanism of FRT10 against obesity might be related to the alterations in glycerophospholipid metabolism, primary bile acid biosynthesis, amino metabolism, and purine and pyrimidine metabolism. Studies on these metabolites could help us better understand the role of FRT10 in obesity induced by HFD.

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“…The amount of lipids accumulated in the cells was measured using Oil Red O (Sigma-Aldrich, St. Louis, MO, USA), which reacts specifically with intracellular lipids. 3T3-L1 adipocyte was measured according to method by Huang et al (2021) . The differentiated cells were washed three times with PBS and fixed with 10% formaldehyde, followed by oil red O solution (stock solution: 3.5 mg/mL in isopropanol; working solution: 60% oil red O stock solution and 40% distilled water) for 30 min at room temperature.…”

Section: Methodsmentioning

confidence: 99%

Probiotic Property and Anti-Obesity Effect of Lactiplantibacillus plantarum KC3

Kim¹,

Huang²,

Ji³

et al. 2022

Food Sci Anim Resour

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Lactic acid bacteria (LAB) are representative probiotics that have beneficial effects on humans.Nineteen strains among the 167 single strains from kimchi was selected and investigated their physiological features. The selection of a strain was based on strong enzyme (lipase, α-amylase and α-glucosidase) inhibitory activities and anti-obesity effects in the adipocytes. For the final selection, the strain Lactiplantibacillus plantarum KC3 was tested for its potential as a starter.To assess its functionality, a freeze-dried culture of L. plantarum KC3 was administered to a diet-induced obese mouse model receiving a high-fat diet. The animal group administered with L. plantarum KC3 showed significant body weight loss during the 12-week feeding period compared to the high-fat control group. This study investigated the physiological characteristics of selected strain and evaluated its potential as an anti-obesity probiotic in mice.

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Modulation of the Gut Microbiome and Obesity Biomarkers by Lactobacillus Plantarum KC28 in a Diet-Induced Obesity Murine Model

Cited by 12 publications

References 69 publications

<i>Lactiplantibacillus plantarum</i> 06CC2 upregulates intestinal ZO-1 protein and bile acid metabolism in Balb/c mice fed high-fat diet

<i>Lactiplantibacillus plantarum</i> 06CC2 upregulates intestinal ZO-1 protein and bile acid metabolism in Balb/c mice fed high-fat diet

Metabolomic Characteristics of Liver and Cecum Contents in High-Fat-Diet-Induced Obese Mice Intervened with Lactobacillus plantarum FRT10

Probiotic Property and Anti-Obesity Effect of Lactiplantibacillus plantarum KC3

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