Modulations of phenotype and cytokine expression of porcine bone marrow-derived dendritic cells by porcine reproductive and respiratory syndrome virus

Peng, Yu-Tang; Chaung, Hso-Chi; HsiuLuan, Chang; Chang, Hsueh-Chen; Chung, Wen-Bin

doi:10.1016/j.vetmic.2008.11.013

Cited by 22 publications

(12 citation statements)

References 25 publications

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“…Interestingly, Poly I:C lacks intrinsic capacity to induce the release of cytokines (at least those evaluated in this study), but it is able to polarize the cytokine profile induced by the antigen. Similarly, it has been reported that this TLR-ligand possesses weak or no stimulatory ability by itself [37,40], but enhances the release of IL-12 by porcine bmDCs stimulated by the bacterial pathogen Haemophilus parasuis [37]. In contrast to swine DCs, human monocyte-derived DCs, purified human CD11c + myeloid DCs, or murine bmDCs produce significant levels of IL-12p40/p70 after Poly I:C stimulation [41,42,43].…”

Section: Discussionmentioning

confidence: 88%

Porcine Dendritic Cells as an In Vitro Model to Assess the Immunological Behaviour of Streptococcus suis Subunit Vaccine Formulations and the Polarizing Effect of Adjuvants

et al. 2017

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An in vitro porcine bone marrow-derived dendritic cell (DC) culture was developed as a model for evaluating immune polarization induced by adjuvants when administered with immunogens that may become vaccine candidates if appropriately formulated. The swine pathogen Streptococcus suis was chosen as a prototype to evaluate proposed S. suis vaccine candidates in combination with the adjuvants Poly I:C, Quil A ®, Alhydrogel ®, TiterMax Gold ® and Stimune ®. The toll-like receptor ligand Poly I:C and the saponin Quil A ® polarized swine DC cytokines towards a type 1 phenotype, with preferential production of IL-12 and TNF-α. The water-in-oil adjuvants TiterMax Gold ® and Stimune ® favoured a type 2 profile as suggested by a marked IL-6 release. In contrast, Alhydrogel ® induced a type 1/type 2 mixed cytokine profile. The antigen type differently modified the magnitude of the adjuvant effect, but overall polarization was preserved. This is the first comparative report on swine DC immune activation by different adjuvants. Although further swine immunization studies would be required to better characterize the induced responses, the herein proposed in vitro model is a promising approach that helps assessing behaviour of the vaccine formulation rapidly at the pre-screening stage and will certainly reduce numbers of animals used while advancing vaccinology science.

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Section: Discussionmentioning

confidence: 88%

Porcine Dendritic Cells as an In Vitro Model to Assess the Immunological Behaviour of Streptococcus suis Subunit Vaccine Formulations and the Polarizing Effect of Adjuvants

et al. 2017

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“…Maturation of DCs is a critical process that results in presenting viral antigens to activate T cells and initiating an antiviral immune response (Zhou et al, 2007). The phenomenon has been reported in the classical swine fever virus (Carrasco et al, 2004) and the PRRS virus (Peng et al, 2009). It is known that DCs present antigen with CD1a or SLA-II-DR as the first signal and CD80/86 as the second costimulatory signal to activate T cells (Bertram et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Effects of virulent and attenuated transmissible gastroenteritis virus on the ability of porcine dendritic cells to sample and present antigen

Song

Gao

Qin

et al. 2014

Veterinary Microbiology

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Virulent transmissible gastroenteritis virus (TGEV) results in an acute, severe pathology and high mortality in piglets, while attenuated TGEV only causes moderate clinical reactions. Dendritic cells (DCs), through uptake and presentation of antigens to T cells, initiate distinct immune responses to different infections. In this study, an attenuated TGEV (STC3) and a virulent TGEV (SHXB) were used to determine whether porcine DCs play an important role in pathogenetic differences between these two TGEVs. Our results showed that immature and mature monocyte-derived dendritic cells (Mo-DCs) were susceptible to infection with SHXB and STC3. However, only SHXB inhibited Mo-DCs to activate T-cell proliferation by down-regulating the expression of cell-surface markers and the secretion of cytokines in vitro. In addition, after 48 h of SHXB infection, there was the impairment in the ability of porcine intestinal DCs to sample the antigen, to migrate from the villi to the lamina propria and to activate T-cell proliferation in vivo. In contrast, these abilities of intestinal DCs were enhanced in STC3-infected piglets. In conclusion, our results show that SHXB significantly impaired the functions of Mo-DCs and intestinal DCs in vitro and in vivo, while STC3 had the opposite effect. These differences may underlie the pathogenesis of virulent and attenuated TGEV in piglets, and could help us to develop a better strategy to prevent virulent TGEV infection.

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“…An accurate review of the scientific literature, however, reveals that every study intended to clarify the effects of the abovementioned interactions produced different results. Some papers indicated that PRRSV downregulates SLA-I and/or SLA-II while some others did not [11,12,21-23] or, for example, CD80/86 have been shown to be up and downregulated by PRRSV [11,12,21]. Similarly, some papers showed that IL-10 and TNF-α are cytokines produced by DC or PAM upon PRRSV infection, while some other papers reported the inability to detect these cytokines [9,11,12,21,23-26].…”

Section: Discussionmentioning

confidence: 99%

“…Nevertheless, it has also been reported that different strains may induce different IL-10 responses in PBMC [3] and, therefore, different outcomes of the infection and the resulting immune response could be expected after infection with different strains. In addition, American-type PRRSV isolates seem to be able to downregulate other important components of the early immune response of antigen presenting cells (APC), in particular major histocompatibility complex (MHC) expression [10,11] and CD80/86 [12]. …”

Section: Introductionmentioning

confidence: 99%

Cytokine profiles and phenotype regulation of antigen presenting cells by genotype-I porcine reproductive and respiratory syndrome virus isolates

Gimeno

Darwich

Díaz

et al. 2011

Vet Res

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The present study examined the immunological response of antigen presenting cells (APC) to genotype-I isolates of porcine reproductive and respiratory syndrome virus (PRRSV) infection by analysing the cytokine profile induced and evaluating the changes taking place upon infection on immunologically relevant cell markers (MHCI, MHCII, CD80/86, CD14, CD16, CD163, CD172a, SWC9). Several types of APC were infected with 39 PRRSV isolates. The results show that different isolates were able to induce different patterns of IL-10 and TNF-α. The four possible phenotypes based on the ability to induce IL-10 and/or TNF-α were observed, although different cell types seemed to have different capabilities. In addition, isolates inducing different cytokine-release profiles on APC could induce different expression of cell markers.

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Modulations of phenotype and cytokine expression of porcine bone marrow-derived dendritic cells by porcine reproductive and respiratory syndrome virus

Cited by 22 publications

References 25 publications

Porcine Dendritic Cells as an In Vitro Model to Assess the Immunological Behaviour of Streptococcus suis Subunit Vaccine Formulations and the Polarizing Effect of Adjuvants

Porcine Dendritic Cells as an In Vitro Model to Assess the Immunological Behaviour of Streptococcus suis Subunit Vaccine Formulations and the Polarizing Effect of Adjuvants

Effects of virulent and attenuated transmissible gastroenteritis virus on the ability of porcine dendritic cells to sample and present antigen

Cytokine profiles and phenotype regulation of antigen presenting cells by genotype-I porcine reproductive and respiratory syndrome virus isolates

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