2006
DOI: 10.1007/s00705-006-0742-y
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Molecular analysis of a California strain of Rupestris stem pitting-associated virus isolated from declining Syrah grapevines

Abstract: The sequence of the genome of a Rupestris stem pitting-associated virus (RSPaV) isolated from a declining Syrah grapevine in California, designated the Syrah strain (RSPaV-SY) was determined. The genome of this strain had an overall nucleotide identity of 77% in comparison with RSPaV sequences in GenBank; the coat protein was the most conserved gene among RSPaV sequences and the replicase was the least conserved gene. Phylogenetic analysis of partial coat protein and replicase gene sequences showed RSPaV-SY cl… Show more

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Cited by 49 publications
(45 citation statements)
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“…110R (V. berlandieri x V. rupestris), not inoculated, exhibiting severe vein necrosis on leaves were sampled for total RNA extractions using the RNeasy Plant Mini kit (Qiagen) from 100mg of petioles of symptomatic leaves, grinding plant tissue in liquid nitrogen. Primer pairs used to amplify the partial CP of GRSPaV for one-step RT-PCR were 48V/49C (LIMA et al, 2006). The RT-PCR in a single step was carried out using One Step RT-PCR kit (Qiagen) and reactions were performed according to the manufacturer with 4μL of total RNA (ca.…”
mentioning
confidence: 99%
“…110R (V. berlandieri x V. rupestris), not inoculated, exhibiting severe vein necrosis on leaves were sampled for total RNA extractions using the RNeasy Plant Mini kit (Qiagen) from 100mg of petioles of symptomatic leaves, grinding plant tissue in liquid nitrogen. Primer pairs used to amplify the partial CP of GRSPaV for one-step RT-PCR were 48V/49C (LIMA et al, 2006). The RT-PCR in a single step was carried out using One Step RT-PCR kit (Qiagen) and reactions were performed according to the manufacturer with 4μL of total RNA (ca.…”
mentioning
confidence: 99%
“…Estudos adicionais de caracterização biológica necessitariam ser conduzidos neste caso. Os oligonucleotídeos específicos para a estirpe Syrah foram desenhados com base no gene da replicase, numa região que apresenta significativa variabilidade, permitindo assim a diferenciação desta estirpe de outras (LIMA et al, 2006 Ciência Rural, v.40, n.11, nov, 2010.…”
Section: Resultsunclassified
“…Para o GLRaV-2, as estirpes consideradas foram: 93/955, PN (=Sem, 94/970), H4, RG, Alfie e BD (ABOU GHANEM-SABANADZOVIC et al, 2000;BERTAZZON & ANGELINI, 2004;MENG et al, 2005a). Em relação ao RSPaV, foram consideradas as estirpes GRSPaV-VS, GRSPaV-BS, GRSPaV-SG1 e GRSPaV-1 (MENG et al, 2006;ALABI et al, 2010) e Syrah (SY) (LIMA et al, 2006) e Pinot Noir (PN) ), recentemente identificadas. Para o GLRaV-3 foi considerada apenas a estirpe "típica" NY (New York) (LING et al, 1997).…”
Section: (Gva) Grapevine Virus B (Gvb) Grapevine Virus D (Gvd) Graunclassified
“…Através da análise da identidade de nt desses isolados de RSPaV, pôde-se verificar a formação de quatro agrupamentos distintos, à semelhança de outros trabalhos (Lima et al 2006;Meng et al, 2006;Nolasco et al, 2006;Nakaune et al 2008 1 -Árvores filogenéticas não enraizadas, obtidas com o programa MEGA 4.1, baseadas nas sequências completas de nucleotídeos do gene da proteína capsidial dos isolados de (A) Rupestris stem pitting associated virus, RSPaV, (B) Grapevine leafroll associated virus 2, GLRaV-2 e (C) Grapevine fanleaf virus, GFLV, com a demarcação de distintos grupos, considerando-se os isolados estudados. Valores percentuais de "bootstrap" são mostrados nos ramos.…”
Section: Rspavunclassified