Molecular analysis of a leu2 ? mutant of Candida maltosa demonstrates the presence of multiple alleles

Becher, Dietmar; Schulze, S.; Kasüske, Anette; Schulze, H; Samsonova, I. A.; Oliver, Stephen G.

doi:10.1007/bf00309549

Cited by 12 publications

(41 citation statements)

References 17 publications

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“…Leucine auxotrophs (4.5% leu from L4) occurred with relatively low frequency, which might be related with the presence of silent gene copies in C. maltosa, as demonstrated recently for the LEU2 gene (Becher et al 1991(Becher et al , 1994. The first C. maltosa leu2 host, J288.…”

Section: Mutant Phenotypesmentioning

confidence: 61%

“…results;Mauersberger 1991). A relatively high instability of some auxotrophic mutants of C. maltosa L4 was observed and analyzed in more detail (APA-change, Schult et al 1987;Becher et al 1994;see Sect. 4…”

Section: Mutagenesis Of Candida Ma/tosamentioning

confidence: 99%

“…Numerous aux mutants of C. maltosa have been isolated mainly from the two wild-type strains, IAM12247 (CBS5611) and SBUG700 (L4), in the Department of Agricultural Chemistry at the University ofTokyo (Chang et al 1984;Hikiji et al 1989;Kawai et al 1991;Ohkuma et al 1993a) and in the Laboratory of Yeast Genetics at the Greifswald University since the beginning of the 1980s Klinner et al 1984;Schwarz et al 1987;Becher et al 1991Becher et al , 1994; cf. -6, his-23 met-6, leu2 ura, leul thi,lys2-21 ino-3, met-13 ino-3), as partially shown in Table 6 (Klinner et al 1984;Schmidt et al 1985;Kunze et al 1987a,b;Samsonova et al 1987;Schwarz et al 1987;Becher et al 1991).…”

Section: Mutant Phenotypesmentioning

confidence: 99%

“…Several of the auxotrophic mutants of C. maltosa induced by UV or MNNG show an unusual behavior (Schult et al 1987;Becher et al 1994). After reversion to prototrophy (nearly one revertant per lOS cells) they mutated to the original auxotrophy, and these auxotrophy-prototrophy-auxotrophy alterations (AP A change) took place successively many times in a cell line.…”

Section: Mutant Phenotypesmentioning

confidence: 99%

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Candida maltosa

Mauersberger

Ohkuma

Schunck

et al. 1996

Nonconventional Yeasts in Biotechnology

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Section: Mutant Phenotypesmentioning

confidence: 61%

Section: Mutagenesis Of Candida Ma/tosamentioning

confidence: 99%

Section: Mutant Phenotypesmentioning

confidence: 99%

Section: Mutant Phenotypesmentioning

confidence: 99%

See 2 more Smart Citations

Candida maltosa

Mauersberger

Ohkuma

Schunck

et al. 1996

Nonconventional Yeasts in Biotechnology

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“…These data are not easily interpreted in terms of known regulatory mechanisms and appear to be at odds with an earlier report that a cloned gene from C. maltosa that showed 76% homology (at the protein sequence level) to LEU2 from S. cerevisiae (4), and was therefore named C-LEU2, was regulated just like LEU2 from S. cerevisiae when transferred into that organism (100). The interpretation of genetic data from C. maltosa is complicated by evidence of a highly aneuploid genome and the spontaneous occurrence of auxotrophy-prototrophy-auxotrophy changes due to the presence of silent gene copies (8).…”

Section: Other Speciesmentioning

confidence: 99%

Leucine Biosynthesis in Fungi: Entering Metabolism through the Back Door

Kohlhaw

2003

Microbiol Mol Biol Rev

221

249

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After exploring evolutionary aspects of branched-chain amino acid biosynthesis, the review focuses on the extended leucine biosynthetic pathway as it operates in Saccharomyces cerevisiae. First, the genes and enzymes specific for the leucine pathway are considered: LEU4 and LEU9 (encoding the α-isopropylmalate synthase isoenzymes), LEU1 (isopropylmalate isomerase), and LEU2 (β-isopropylmalate dehydrogenase). Emphasis is given to the unusual distribution of the branched-chain amino acid pathway enzymes between mitochondrial matrix and cytosol, on the newly defined role of Leu5p, and on regulatory mechanisms governing gene expression and enzyme activity, including new evidence for the metabolic importance of the regulation of α-isopropylmalate synthase by coenzyme A. Next, structure-function relationships of the transcriptional regulator Leu3p are addressed, defining its dual role as activator and repressor and discussing evidence in support of the self-masking model. Recent data pointing at a more extended Leu3p regulon are discussed. An overview of the layered controls of the extended leucine pathway is provided that includes a description of the newly recognized roles of Ilv5p and Bat1p in maintaining mitochondrial integrity. Finally, branched-chain amino acid biosynthesis and its regulation in other fungi are summarized, the question of leucine as metabolic signal is addressed, and possible directions of future research in this area are outlined

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TheSPL1 tRNA splicing gene ofCandida maltosa andCandida albicans

Plant¹,

Becher²,

Poulter³

1998

Yeast

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The tRNA splicing gene SPL1‐1 has been cloned and sequenced in Saccharomyces cerevisiae (Kolman and Soll, 1993). Sequence adjacent to the LEU2 gene in Candida maltosa showed some homology to the SPL1‐1 gene of S. cerevisiae. This work describes the sequencing of the SPL1 tRNA splicing genes from C. maltosa and C. albicans and the analysis of these genes. Comparison of these sequences and the relationship observed between the LEU2 and SPL1 genes in these yeasts suggests that there may be some synteny amongst various species of yeasts. The coding region of the C. maltosa SPL1 region described in this work differs from previously described partial sequences in that it is a complete uninterrupted open reading frame. Two strains of C. maltosa were each shown to contain different alleles, one uninterrupted open reading frame and one disrupted open reading frame. The sequences have been deposited in the GenBank/EMBL data libraries under Accession Numbers X72940, AF000115, AF000116, AF000117, AF000118, AF000119 and AF000120. © 1998 John Wiley & Sons, Ltd.

show abstract

Molecular analysis of a leu2 ? mutant of Candida maltosa demonstrates the presence of multiple alleles

Cited by 12 publications

References 17 publications

Candida maltosa

Candida maltosa

Leucine Biosynthesis in Fungi: Entering Metabolism through the Back Door

TheSPL1 tRNA splicing gene ofCandida maltosa andCandida albicans

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